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Open Access 01-09-2018 | Original Article

Effects of a sodium fluoride- and phytate-containing dentifrice on remineralisation of enamel erosive lesions—an in situ randomised clinical study

Authors: Jonathan E. Creeth, Charles R. Parkinson, Gary R. Burnett, Susmita Sanyal, Frank Lippert, Domenick T. Zero, Anderson T. Hara

Published in: Clinical Oral Investigations | Issue 7/2018

Abstract

Objective

The objective of this work was to evaluate effects of a dentifrice containing sodium fluoride (1150 ppm F) and the organic polyphosphate phytate (0.85% w/w of the hexa-sodium salt) on in situ remineralisation of early enamel erosive lesions and resistance to subsequent demineralisation.

Materials and methods

Subjects (n = 62) wore palatal appliances holding eight bovine enamel specimens with pre-formed erosive lesions. They brushed their natural teeth with the phytate test dentifrice (TD); a positive control dentifrice (PC, 1150 ppm fluoride as NaF); a reference dentifrice (RD, disodium pyrophosphate + 1100 ppm fluoride as NaF) or a negative control dentifrice (NC, fluoride-free) in a randomised, double-blind, crossover design. Specimens were removed at 2, 4 and 8 h post-brushing and exposed to an ex vivo acid challenge. Surface microhardness (Knoop) was measured at each stage. The primary efficacy variable was relative erosion resistance (RER); other variables included the surface microhardness recovery (SMHR), acid resistance ratio (ARR) and enamel fluoride uptake (EFU).

Results

After 4 h, the results for RER, ARR and EFU were in the order PC > TD = RD > NC with PC > TD = RD = NC for SMHR. Results at 2 and 8 h were generally consistent with the 4 h data. Mineralisation progressed over time. Dentifrices were generally well-tolerated.

Conclusions

In this in situ model, addition of phytate or pyrophosphate to a fluoride dentifrice inhibited the remineralising effect of fluoride. Both formulations still delivered fluoride to the enamel and inhibited demineralisation, albeit to a lesser extent than a polyphosphate-free dentifrice.

Clinical relevance

Addition of phytate or pyrophosphate to a fluoride dentifrice may reduce its net anti-erosive properties.

Imfeld T (1996) Dental erosion. Definition, classification and links. Eur J Oral Sci 104(2):151–155. https://doi.org/10.1111/j.1600-0722.1996.tb00063.x CrossRefPubMed

Attin T, Buchalla W, Gollner M, Hellwig E (2000) Use of variable remineralization periods to improve the abrasion resistance of previously eroded enamel. Caries Res 34(1):48–52. https://doi.org/10.1159/000016569 CrossRefPubMed

Eisenburger M, Shellis RP, Addy M (2003) Comparative study of wear of enamel induced by alternating and simultaneous combinations of abrasion and erosion in vitro. Caries Res 37(6):450–455. https://doi.org/10.1159/000073399 CrossRefPubMed

Gedalia I, Ionat-Bendat D, Ben-Mosheh S, Shapira L (1991) Tooth enamel softening with a cola type drink and rehardening with hard cheese or stimulated saliva in situ. J Oral Rehab 18(6):501–506. https://doi.org/10.1111/j.1365-2842.1991.tb00072.x CrossRef

Amaechi BT, Higham SM (2001) In vitro remineralisation of eroded enamel lesions by saliva. J Dent 29(5):371–376. https://doi.org/10.1016/S0300-5712(01)00026-4 CrossRefPubMed

Zero DT, Hara AT, Kelly SA, González-Cabezas C, Eckert GJ, Barlow AP, Mason SC (2006) Evaluation of a desensitizing test dentifrice using an in situ erosion remineralization model. J Clin Dent 17(4):112–116PubMed

Barlow AP, Sufi F, Mason SC (2009) Evaluation of different fluoridated dentifrice formulations using an in situ erosion remineralization model. J Clin Dent 20:192–198PubMed

Creeth JE, Kelly SA, Martinez-Mier EA, Hara AT, Bosma ML, Butler A, Lynch RJ, Zero DT (2015) Dose-response effect of fluoride dentifrice on remineralisation and further demineralisation of erosive lesions: a randomised in situ clinical study. J Dent 43(7):823–831. https://doi.org/10.1016/j.jdent.2015.03.008 CrossRefPubMed

Wűlknitz P (1997) Cleaning power and abrasivity of European toothpastes. Adv Dent Res 11(4):576–579. https://doi.org/10.1177/08959374970110042701 CrossRefPubMed

10.

Schemehorn BR, Moore MH, Putt MS (2011) Abrasion, polishing, and stain removal characteristics of various commercial dentifrices in vitro. J Clin Dent 22(1):11–18PubMed

11.

Baig A, Kozak K, Cox ER, Zoladz J, Mahony L, White D (2002) Laboratory studies on the chemical whitening effects of a sodium hexametaphosphate dentifrice. J Clin Dent 13(1):19–24PubMed

12.

Joiner A (2010) Whitening toothpastes: a review of the literature. J Dent 38;Suppl 2:e17–24

13.

Featherstone JD, Shariati M, Brugler S, Fu J, White DJ (1988) Effect of an anticalculus dentifrice on lesion progression under pH cycling conditions in vitro. Caries Res 22(6):337–341. https://doi.org/10.1159/000261133 CrossRefPubMed

14.

Lu KH, Yen DJ, Zacherl WA, Ruhlman CD, Sturzenberger OP, Lehnhoff RW (1985) The effect of a fluoride dentifrice containing an anticalculus agent on dental caries in children. ASDC J Dent Child 52(6):449–451PubMed

15.

Ripa LW, Leske GS, Triol CW, Volpe AR (1990) Clinical study of the anticaries efficacy of three fluoride dentifrices containing anticalculus ingredients: three-year (final) results. J Clin Dent 2(2):29–33PubMed

16.

Zero DT, Cavaretta Siegel G, Fu J, Li H (2000) Effect of pyrophosphate on fluoride enhanced remineralization after an erosive challenge. Caries Res 34:344 Abstract 105

17.

Creeth JE, Zero DT, Kelly SA, Martinez-Mier EA, Hara A, Bosma ML, Newby EE (2014) Relative remineralisation potential of sodium fluoride dentifrices in situ. J Dent Res 93 (Sp Iss A): Abstract 1339

18.

Magrill DS (1973) The reduction of the solubility of hydroxyapatite in acid by adsorption of phytate from solution. Arch Oral Biol 18(5):591–600. https://doi.org/10.1016/0003-9969(73)90097-6 CrossRefPubMed

19.

Vogel GL, Mao Y, Carey CM, Chow LC (1997) Changes in the permselectivity of human teeth during caries attack. J Dent Res 76(2):673–681. https://doi.org/10.1177/00220345970760020801 CrossRefPubMed

20.

McClure FJ (1964) Cariostatic effects of phosphates. Science 114:1337–1338CrossRef

21.

Grases F, Perelló J, Sanchis P, Isern B, Prieto RM, Costa-Bauzá A, Santiago C, Ferragut ML, Frontera G (2009) Anticalculus effect of a triclosan mouthwash containing phytate: a double-blind, randomized, three-period crossover trial. J Periodontal Res 44(5):616–621. https://doi.org/10.1111/j.1600-0765.2008.01168.x CrossRefPubMed

22.

Nehme M, Jeffrey P, Mason S, Lippert F, Zero DT, Hara AT (2016) Erosion remineralization efficacy of gel-to-foam fluoride toothpastes in situ: a randomized clinical trial. Caries Res 50(1):62–70. https://doi.org/10.1159/000443187 CrossRefPubMed

23.

Parkinson CR, Nehme M, Horton J, Hara AT, Zero D (2016) A modified in-situ model to evaluate the anti-erosion properties of a fluoride dentifrice. Caries Res 50:247 Abstract 150

24.

Zero DT, Fu J, Anne KM, Cassata S, McCormack SM, Gwinner LM (1992) An improved intra-oral enamel demineralization test model for the study of dental caries. J Dent Res 71:871–878CrossRefPubMed

25.

Hara AT, Kelly SA, González-Cabezas C, Eckert GJ, Barlow AP, Mason SC, Zero DT (2009) Influence of fluoride availability of dentifrices on eroded enamel remineralization in situ. Caries Res 43(1):57–63. https://doi.org/10.1159/000201591 CrossRefPubMed

26.

Gelhard TB, ten Cate JM, Arends J (1979) Rehardening of artificial enamel lesions in vivo. Caries Res 13(2):80–83. https://doi.org/10.1159/000260387 CrossRefPubMed

27.

Corpron RE, Clark JW, Tsai A, More FG, Merrill DF, Kowalski CJ, Tice TR, Rowe CE (1986) Intraoral effects of a fluoride-releasing device on acid-softened enamel. J Am Dent Assoc 113(3):383–388. https://doi.org/10.14219/jada.archive.1986.0202 CrossRefPubMed

28.

Sakkab NY, Cilley WA, Haberman JP (1984) Fluoride in deciduous teeth from an anti-caries clinical study. J Dent Res 63(10):1201–1205. https://doi.org/10.1177/00220345840630100601 CrossRefPubMed

29.

Lippert F, Butler A, Lynch RJM (2013) Characteristics of methylcellulose acid gel lesions created in human and bovine enamel. Caries Res 47:50–55CrossRefPubMed

30.

Lippert F, Juthani K (2015) Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions. Clin Oral Invest 19(8):1947–1954. https://doi.org/10.1007/s00784-015-1436-1 CrossRef

31.

Hara AT, Gonzalez-Cabezas C, Creeth JE, Parmar M, Eckert GJ, Zero DT (2009) Interplay between fluoride and abrasivity of dentifrices on dental erosion–abrasion. J Dent 37(10):781–785. https://doi.org/10.1016/j.jdent.2009.06.006 CrossRefPubMed

32.

Featherstone JD (1999) Prevention and reversal of dental caries: role of low level fluoride. Community Dent Oral Epidemiol 27(1):31–40. https://doi.org/10.1111/j.1600-0528.1999.tb01989.x CrossRefPubMed

33.

Duckworth RM, Morgan SM (1991) Oral retention of fluoride after use of fluoride dentifrices. Caries Res 25(2):123–129. https://doi.org/10.1159/000261354 CrossRefPubMed

34.

White DJ, Nelson DGA, Faller RV (1994) Mode of action of fluoride: application of new techniques and test methods to the examination of the mechanism of action of topical fluoride. Adv Dent Res 8(2):166–174. https://doi.org/10.1177/08959374940080020601 CrossRefPubMed

35.

Petzold M (2001) The influence of different fluoride compounds and treatment conditions on dental enamel: a descriptive in vitro study of the CaF2 precipitation and microstructure. Caries Res 35(suppl 1):45–51. https://doi.org/10.1159/000049110 CrossRefPubMed

Title: Effects of a sodium fluoride- and phytate-containing dentifrice on remineralisation of enamel erosive lesions—an in situ randomised clinical study
Authors: Jonathan E. Creeth
Charles R. Parkinson
Gary R. Burnett
Susmita Sanyal
Frank Lippert
Domenick T. Zero
Anderson T. Hara
Publication date: 01-09-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2351-z

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effects of a sodium fluoride- and phytate-containing dentifrice on remineralisation of enamel erosive lesions—an in situ randomised clinical study

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

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