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01-04-2018 | Original Article

Bacterial penetration into filled root canals exposed to different pressures and to the oral environment—in vivo analysis

Authors: Ana Maria Hecke Alves, Maria Helena Pozzobon, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Beatriz Dulcinéia Mendes Souza, Lucas da Fonseca Roberti Garcia, Wilson Tadeu Felippe

Published in: Clinical Oral Investigations | Issue 3/2018

Abstract

Objectives

The study aims to correlate the depth of bacterial penetration into filled root canals with the time of exposure to the oral environment and different pressures.

Materials and methods

One-hundred and twenty-two root canals of male Beagle dog teeth were prepared and filled. The root canals were distributed into three groups, according to the pressure applied: (A) no pressure, (B) 30 kPa, and (C) 60 kPa. Then, the root canals were exposed to the oral environment, establishing sub-groups considering the time intervals of exposure: (1) 45 days and (2) 120 days (n = 17). Sub-groups had positive and negative controls (n = 5). The animals were sacrificed, and the specimens were prepared for histological analysis.

Results

There was no significant difference in the bacterial penetration among groups A, B, and C at 45 days (P = 0.903) and 120 days (P = 0.211). No statistically significant difference was found (P = 0.608) between the exposure time intervals. Most of the specimens from experimental groups exposed for 120 days presented moderate inflammatory infiltrate.

Conclusions

Pressures of 30 and 60 kPa did not affect sealing ability of root canal filling. The time of exposure did not influence bacterial penetration, which was limited to the first 4 mm of the root canals exposed for 120 days.

Clinical relevance

This animal study demonstrated that disinfection of the first millimeters of root canals could be considered before retreatment of their entire length. However, clinical studies in humans should be conducted before validation of this protocol.

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Title: Bacterial penetration into filled root canals exposed to different pressures and to the oral environment—in vivo analysis
Authors: Ana Maria Hecke Alves
Maria Helena Pozzobon
Eduardo Antunes Bortoluzzi
Cleonice da Silveira Teixeira
Beatriz Dulcinéia Mendes Souza
Lucas da Fonseca Roberti Garcia
Wilson Tadeu Felippe
Publication date: 01-04-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 3/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2199-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Bacterial penetration into filled root canals exposed to different pressures and to the oral environment—in vivo analysis

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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