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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2018

01-03-2018 | Original Article

Expression of small leucine-rich extracellular matrix proteoglycans biglycan and lumican reveals oral lichen planus malignant potential

Authors: Božana Lončar-Brzak, Marko Klobučar, Irena Veliki-Dalić, Ivan Sabol, Sandra Kraljević Pavelić, Božo Krušlin, Marinka Mravak-Stipetić

Published in: Clinical Oral Investigations | Issue 2/2018

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Abstract

Objectives

The aim of this study was to examine molecular alterations on the protein level in lesions of oral lichen planus (OLP), oral squamous cell carcinoma (OSCC) and healthy mucosa.

Materials and methods

Global protein profiling methods based on liquid chromatography coupled to mass spectrometry (LC-MS) were used, with a special emphasis on evaluation of deregulated extracellular matrix molecules expression, as well as on analyses of IG2F and IGFR2 expression in healthy mucosa, OLP and OSCC tissues by comparative semi-quantitative immunohistochemistry.

Results

Mass spectrometry-based proteomics profiling of healthy mucosa, OLP and OSCC tissues (and accompanied histologically unaltered tissues, respectively) identified 55 extracellular matrix proteins. Twenty among identified proteins were common to all groups of samples. Expression of small leucine-rich extracellular matrix proteoglycans lumican and biglycan was found both in OSCC and OLP and they were validated by Western blot analysis as putative biomarkers. A significant increase (p < 0.05) of biglycan expression in OLP-AT group was determined in comparison with OLP-T group, while lumican showed significant up-regulation (p < 0.05) in OLP-T and OSCC-T groups vs. adjacent and control tissue groups. Biglycan expression was only determined in OSCC-AT group. Immunohistochemical analysis of IGF2 and IG2FR expression revealed no significant difference among groups of samples.

Conclusion/clinical relevance

Biglycan and lumican were identified as important pathogenesis biomarkers of OLP that point to its malignant potential.
Appendix
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Metadata
Title
Expression of small leucine-rich extracellular matrix proteoglycans biglycan and lumican reveals oral lichen planus malignant potential
Authors
Božana Lončar-Brzak
Marko Klobučar
Irena Veliki-Dalić
Ivan Sabol
Sandra Kraljević Pavelić
Božo Krušlin
Marinka Mravak-Stipetić
Publication date
01-03-2018
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 2/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-017-2190-3

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