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01-03-2018 | Original Article

Volatile organic compounds in the breath of oral candidiasis patients: a pilot study

Authors: Moritz Hertel, Eyke Schuette, Isabell Kastner, Stefan Hartwig, Andrea Maria Schmidt-Westhausen, Robert Preissner, Sebastian Paris, Saskia Preissner

Published in: Clinical Oral Investigations | Issue 2/2018

Abstract

Objectives

The aim of the study was to investigate whether specific volatile organic compounds (VOCs) can be detected in oral candidiasis patients using breath analysis in order to develop a point-of-care diagnostic tool.

Patients/methods

Breath samples of 10 diseased patients and 10 subjects carrying no Candida spp. were analyzed using gas chromatography and mass spectrometry. In infected patients, breath tests were performed before and after antifungal therapy.

Results

Breath testing was positive for 143 volatiles in both healthy subjects and diseased patients. Among those, specific signature volatiles known to be emitted by Candida spp. in vitro were not detected. Even though no specific signature was retrieved from the diseased patients, a pattern containing nine compounds (2-methyl-2-butanol, hexanal, longifolene, methyl acetate, 1-heptene, acetophenone, decane, 3-methyl-1-butanol, chlorbenzene) was identified, which showed characteristic changes after antifungal therapy.

Conclusions

Focusing on the identified pattern, breath analysis may be applied to confirm the absence of Candida spp. after therapy in terms of a confirmatory test supplementing clinical examination, thereby replacing microbial testing. However, microbial testing will still be needed to initially confirm clinical diagnoses, as no specific signature was found.

Clinical relevance: A breath test may help in avoiding extended antifungal administration resulting in resistance development and might be useful in the monitoring of disease recurrences in vulnerable groups.

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Title: Volatile organic compounds in the breath of oral candidiasis patients: a pilot study
Authors: Moritz Hertel
Eyke Schuette
Isabell Kastner
Stefan Hartwig
Andrea Maria Schmidt-Westhausen
Robert Preissner
Sebastian Paris
Saskia Preissner
Publication date: 01-03-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2147-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Volatile organic compounds in the breath of oral candidiasis patients: a pilot study

Abstract

Objectives

Patients/methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Patients/methods

Results

Conclusions

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