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01-09-2017 | Original Article

Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Authors: Richard J. Miron, Masako Fujioka-Kobayashi, Yufeng Zhang, Anton Sculean, Benjamin Pippenger, Yoshinori Shirakata, Umadevi Kandalam, Maria Hernandez

Published in: Clinical Oral Investigations | Issue 7/2017

Abstract

Objectives

Dimensional changes of the alveolar bone following tooth extraction are a major challenge in daily dental practice. To limit bone loss, a variety of biomaterials including bone grafts, barrier membranes, and growth factors have been utilized either alone or in combination therapies to increase the speed and quality of new bone formation. The aim of the present in vitro study was to investigate the regenerative potential of Osteogain®, a new liquid carrier system of enamel matrix derivative (EMD) in combination with an absorbable collagen sponge (ACS) specifically designed for extraction socket healing.

Materials and methods

The potential of ACS was first investigated using ELISA to quantify total amelogenin adsorption and release from 0 to 10 days. Thereafter, the cellular effects of ST2 pre-osteoblasts were investigated for cellular attachment at 8 h and cell proliferation at 1, 3, and 5 days as well as osteoblast differentiation by real-time PCR and alizarin red staining for cells seeded on (1) tissue culture plastic, (2) ACS alone, and (3) ACS + Osteogain®.

Results

ACS efficiently loaded nearly 100% of the amelogenin proteins found in Osteogain® which were gradually released up to a 10-day period. Osteogain® also significantly induced a 1.5-fold increase in cell attachment and resulted in a 2–6-fold increase in mRNA levels of osteoblast differentiation markers including runt-related transcription factor 2 (Runx2), collagen1a2, alkaline phosphatase, and bone sialoprotein as well as induced alizarin red staining when combined with ACS.

Conclusions

In summary, these findings suggest that Osteogain® is capable of inducing osteoblast attachment and differentiation when combined with ACS. Future animal studies and randomized human clinical trials are necessary to further support these findings.

Clinical relevance

The use of Osteogain® in combination with ACS may provide a valuable means to limit dimensional changes following tooth extraction.

Johnson K (1963) A study of the dimensional changes occurring in the maxilla after tooth extraction.—part I. Normal healing. Aust Dent J 8:428–433CrossRef

Araújo MG, Lindhe J (2005) Dimensional ridge alterations following tooth extraction. An experimental study in the dog. J Clin Periodontol 32:212–218CrossRefPubMed

Chappuis V, Engel O, Reyes M, Shahim K, Nolte LP, Buser D (2013) Ridge alterations post-extraction in the esthetic zone: a 3D analysis with CBCT. J Dent Res 92:195s–201s. doi:10.1177/0022034513506713 CrossRefPubMedPubMedCentral

Brkovic BM, Prasad HS, Konandreas G, Milan R, Antunovic D, Sandor GK, Rohrer MD (2008) Simple preservation of a maxillary extraction socket using beta-tricalcium phosphate with type I collagen: preliminary clinical and histomorphometric observations. J (Canadian Dental Association) 74:523–528

Brkovic BM, Prasad HS, Rohrer MD, Konandreas G, Agrogiannis G, Antunovic D, Sandor GK (2012) Beta-tricalcium phosphate/type I collagen cones with or without a barrier membrane in human extraction socket healing: clinical, histologic, histomorphometric, and immunohistochemical evaluation. Clin Oral Invest 16:581–590. doi:10.1007/s00784-011-0531-1 CrossRef

Mardas N, Chadha V, Donos N (2010) Alveolar ridge preservation with guided bone regeneration and a synthetic bone substitute or a bovine-derived xenograft: a randomized, controlled clinical trial. Clin Oral Implants Res 21:688–698. doi:10.1111/j.1600-0501.2010.01918.x CrossRefPubMed

Wallace S (2015) Histomorphometric and 3D cone-beam computerized tomographic evaluation of socket preservation in molar extraction sites using human particulate mineralized cancellous allograft bone with a porcine collagen xenograft barrier: a case series. J Oral Implantol 41:291–297. doi:10.1563/aaid-joi-D-14-00078 CrossRefPubMed

Bayat M, Momen Heravi F, Mahmoudi M, Bahrami N (2015) Bone reconstruction following application of bone matrix gelatin to alveolar defects: a randomized clinical trial. Int J Organ Transplant Med 6:176–181PubMedPubMedCentral

Mardas N, D'Aiuto F, Mezzomo L, Arzoumanidi M, Donos N (2011) Radiographic alveolar bone changes following ridge preservation with two different biomaterials. Clin Oral Implants Res 22:416–423. doi:10.1111/j.1600-0501.2010.02154.x CrossRefPubMed

10.

Coomes AM, Mealey BL, Huynh-Ba G, Barboza-Arguello C, Moore WS, Cochran DL (2014) Buccal bone formation after flapless extraction: a randomized, controlled clinical trial comparing recombinant human bone morphogenetic protein 2/absorbable collagen carrier and collagen sponge alone. J Periodontol 85:525–535. doi:10.1902/jop.2013.130207 CrossRefPubMed

11.

Fiorellini JP, Howell TH, Cochran D, Malmquist J, Lilly LC, Spagnoli D, Toljanic J, Jones A, Nevins M (2005) Randomized study evaluating recombinant human bone morphogenetic protein-2 for extraction socket augmentation. J Periodontol 76:605–613. doi:10.1902/jop.2005.76.4.605 CrossRefPubMed

12.

Misch CM (2010) The use of recombinant human bone morphogenetic protein-2 for the repair of extraction socket defects: a technical modification and case series report. Int J Oral Maxillofac Implants 25:1246–1252PubMed

13.

Wallace SC, Pikos MA, Prasad H (2014) De novo bone regeneration in human extraction sites using recombinant human bone morphogenetic protein-2/ACS: a clinical, histomorphometric, densitometric, and 3-dimensional cone-beam computerized tomographic scan evaluation. Implant Dent 23:132–137. doi:10.1097/id.0000000000000035 CrossRefPubMed

14.

De Risi V, Clementini M, Vittorini G, Mannocci A, De Sanctis M (2015) Alveolar ridge preservation techniques: a systematic review and meta-analysis of histological and histomorphometrical data. Clin Oral Implants Res 26:50–68. doi:10.1111/clr.12288 CrossRefPubMed

15.

Jambhekar S, Kernen F, Bidra AS (2015) Clinical and histologic outcomes of socket grafting after flapless tooth extraction: a systematic review of randomized controlled clinical trials. J Prosthet Dent 113:371–382. doi:10.1016/j.prosdent.2014.12.009 CrossRefPubMed

16.

Moraschini V, Barboza ED (2016) Quality assessment of systematic reviews on alveolar socket preservation. Int J Oral Maxillofac Surg. doi:10.1016/j.ijom.2016.03.010

17.

Spagnoli D, Choi C (2013) Extraction socket grafting and buccal wall regeneration with recombinant human bone morphogenetic protein-2 and acellular collagen sponge. Atlas Oral Maxillofac Surg Clin North Am 21:175–183. doi:10.1016/j.cxom.2013.05.003 CrossRefPubMed

18.

Morjaria KR, Wilson R, Palmer RM (2014) Bone healing after tooth extraction with or without an intervention: a systematic review of randomized controlled trials. Clin Implant Dent Relat Res 16:1–20. doi:10.1111/j.1708-8208.2012.00450.x CrossRefPubMed

19.

Tan WL, Wong TL, Wong MC, Lang NP (2012) A systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans. Clin Oral Implants Res 23(Suppl 5):1–21. doi:10.1111/j.1600-0501.2011.02375.x CrossRefPubMed

20.

De Sarkar A, Singhvi N, Shetty JN, Ramakrishna T, Shetye O, Islam M, Keerthy H (2015) The local effect of alendronate with intra-alveolar collagen sponges on post extraction alveolar ridge resorption: a clinical trial. J Maxillofac Oral Surg 14:344–356. doi:10.1007/s12663-014-0633-9 CrossRefPubMed

21.

Zhang Y, Yang S, Zhou W, Fu H, Qian L, Miron RJ (2015) Addition of a synthetically fabricated osteoinductive biphasic calcium phosphate bone graft to BMP2 improves new bone formation. Clin Implant Dent Relat Res. doi:10.1111/cid.12384

22.

Miron RJ, Bosshardt DD, Buser D, Zhang Y, Tugulu S, Gemperli A, Dard M, Caluseru OM, Chandad F, Sculean A (2015) Comparison of the capacity of enamel matrix derivative gel and enamel matrix derivative in liquid formulation to adsorb to bone grafting materials. J Periodontol 86:578–587. doi:10.1902/jop.2015.140538 CrossRefPubMed

23.

Miron RJ, Chandad F, Buser D, Sculean A, Cochran DL, Zhang Y (2016) Effect of enamel matrix derivative liquid on osteoblast and periodontal ligament cell proliferation and differentiation. J Periodontol 87:91–99. doi:10.1902/jop.2015.150389 CrossRefPubMed

24.

Zhang Y, Jing D, Buser D, Sculean A, Chandad F, Miron RJ (2016) Bone grafting material in combination with Osteogain for bone repair: a rat histomorphometric study. Clin Oral Invest 20:589–595. doi:10.1007/s00784-015-1532-2 CrossRef

25.

Miron RJ, Sculean A, Cochran DL, Froum S, Zucchelli G, Nemcovsky C, Donos N, Lyngstadaas SP, Deschner J, Dard M, Stavropoulos A, Zhang Y, Trombelli L, Kasaj A, Shirakata Y, Cortellini P, Tonetti M, Rasperini G, Jepsen S, Bosshardt DD (2016) 20 years of enamel matrix derivative: the past, the present and the future. J Clin Periodontol. doi:10.1111/jcpe.12546 PubMed

26.

Boyan BD, Weesner TC, Lohmann CH, Andreacchio D, Carnes DL, Dean DD, Cochran DL, Schwartz Z (2000) Porcine fetal enamel matrix derivative enhances bone formation induced by demineralized freeze dried bone allograft in vivo. J Periodontol 71:1278–1286. doi:10.1902/jop.2000.71.8.1278 CrossRefPubMed

27.

Kauvar AS, Thoma DS, Carnes DL, Cochran DL (2010) In vivo angiogenic activity of enamel matrix derivative. J Periodontol 81:1196–1201. doi:10.1902/jop.2010.090441 CrossRefPubMed

28.

Neeley WW, Carnes DL, Cochran DL (2010) Osteogenesis in an in vitro coculture of human periodontal ligament fibroblasts and human microvascular endothelial cells. J Periodontol 81:139–149. doi:10.1902/jop.2009.090027 CrossRefPubMed

29.

Schlueter SR, Carnes DL, Cochran DL (2007) In vitro effects of enamel matrix derivative on microvascular cells. J Periodontol 78:141–151. doi:10.1902/jop.2007.060111 CrossRefPubMed

30.

Thoma DS, Villar CC, Carnes DL, Dard M, Chun YH, Cochran DL (2011) Angiogenic activity of an enamel matrix derivative (EMD) and EMD-derived proteins: an experimental study in mice. J Clin Periodontol 38:253–260. doi:10.1111/j.1600-051X.2010.01656.x CrossRefPubMed

31.

Miron RJ, Hedbom E, Saulacic N, Zhang Y, Sculean A, Bosshardt DD, Buser D (2011) Osteogenic potential of autogenous bone grafts harvested with four different surgical techniques. J Dent Res 90:1428–1433. doi:10.1177/0022034511422718 CrossRefPubMed

32.

Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101–1108CrossRefPubMed

33.

Hoang AM, Klebe RJ, Steffensen B, Ryu OH, Simmer JP, Cochran DL (2002) Amelogenin is a cell adhesion protein. J Dent Res 81:497–500CrossRefPubMed

34.

Quarles LD, Yohay DA, Lever LW, Caton R, Wenstrup RJ (1992) Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7:683–692. doi:10.1002/jbmr.5650070613 CrossRefPubMed

35.

Miron RJ, Bosshardt DD, Hedbom E, Zhang Y, Haenni B, Buser D, Sculean A (2012) Adsorption of enamel matrix proteins to a bovine-derived bone grafting material and its regulation of cell adhesion, proliferation, and differentiation. J Periodontol 83:936–947. doi:10.1902/jop.2011.110480 CrossRefPubMed

36.

Miron RJ, Caluseru OM, Guillemette V, Zhang Y, Buser D, Chandad F, Sculean A (2015) Effect of bone graft density on in vitro cell behavior with enamel matrix derivative. Clin Oral Invest 19:1643–1651. doi:10.1007/s00784-014-1388-x CrossRef

37.

Miron RJ, Fujioka-Kobayashi M, Zhang Y, Caballe-Serrano J, Shirakata Y, Bosshardt DD, Buser D, Sculean A (2016) Osteogain improves osteoblast adhesion, proliferation and differentiation on a bovine-derived natural bone mineral. Clin Oral Implants Res. doi:10.1111/clr.12802

Title: Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro
Authors: Richard J. Miron
Masako Fujioka-Kobayashi
Yufeng Zhang
Anton Sculean
Benjamin Pippenger
Yoshinori Shirakata
Umadevi Kandalam
Maria Hernandez
Publication date: 01-09-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-2019-5

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Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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