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Published in:

01-09-2017 | Original Article

Final irrigation protocols may affect intraradicular dentin ultrastructure

Authors: Márcia Helena Wagner, Ricardo Abreu da Rosa, José Antonio Poli de Figueiredo, Marco Antonio Húngaro Duarte, Jefferson Ricardo Pereira, Marcus Vinicius Reis Só

Published in: Clinical Oral Investigations | Issue 7/2017

Abstract

Objectives

The aim of this study was to evaluate the effect of different irrigation protocols on the root dentin structure using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Materials and methods

Thirty-nine lower bovine incisors were hemisected longitudinally and randomly divided into 13 groups (n = 3). After the root halves were reassembled, it was applied a specific irrigation protocol for each group, as following: G1, distilled water (control); G2, 0.9% saline; G3, saline + 17% EDTA; G4, saline + PUI; G5, saline + PUI + EDTA; G6 to G9 received the same protocol as above replacing 0.9% saline by 2.5% NaOCl; and G10 to G13 by 2% CHX. One-half of each sample was prepared and evaluated using SEM and the other one by TEM observations.

Results

TEM descriptive analysis showed modifications in dentin organic ultrastructure, characterized by the thinning of dentin collagen fibrils, caused by NaOCl, enhanced by EDTA and/or PUI. SEM analysis showed that NaOCl with PUI caused significantly larger erosion of the peritubular dentin than in all the other groups (P < 0.05), followed by NaOCl + EDTA and NaOCl + EDTA + PUI.

Conclusions

NaOCl caused ultrastructural alterations in the dentin collagen, and enhanced by EDTA and/or PUI, promoted peritubular and intertubular erosion.

Clinical relevance

The effect of irrigating solutions on dentin ultrastructure was still unclear. The acknowledgment about the kind of solution, concentrations, application time, and sequence of use was important to achieve the right sanitization without jeopardizing the dentin ultrastructure quality.

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Title: Final irrigation protocols may affect intraradicular dentin ultrastructure
Authors: Márcia Helena Wagner
Ricardo Abreu da Rosa
José Antonio Poli de Figueiredo
Marco Antonio Húngaro Duarte
Jefferson Ricardo Pereira
Marcus Vinicius Reis Só
Publication date: 01-09-2017
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-2006-x

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Final irrigation protocols may affect intraradicular dentin ultrastructure

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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