Top

Clinical Oral Investigations

Published in:

01-11-2016 | Original Article

In vitro salivary pellicles from adults and children have different protective effects against erosion

Authors: Thiago S. Carvalho, Tommy Baumann, Adrian Lussi

Published in: Clinical Oral Investigations | Issue 8/2016

Abstract

Objectives

We aimed at analyzing the protective effects of salivary pellicles, formed with saliva from adults or children, on enamel from permanent or deciduous teeth.

Materials and methods

Ninety human enamel specimens (45 permanent premolars and 45 deciduous canines) were ground, and the outer 200 μm of enamel was removed. We divided the teeth into three further subgroups: no salivary pellicle (control), adult salivary pellicle (AP), and child salivary pellicle (CP). We collected stimulated saliva from adults and children and placed 160 μl of either saliva on enamel specimens from AP and CP, respectively. Control specimens received no saliva. Specimens were stored at 37 °C for 2 h and then submitted to an erosive challenge (10 mL; 1 % citric acid; pH 3.6; 25 °C, 1 min). Pellicle formation and erosion was repeated for a total of 4 cycles. After every cycle, relative surface reflection intensity (rSRI) and surface microhardness (rSMH) were calculated.

Results

On permanent enamel, AP presented significantly better protective effects, with less rSMH loss (p < 0.001) and less rSRI loss (p < 0.001). On deciduous enamel, CP presented significantly better protective effects than AP and control (p < 0.05), for both measured parameters.

Conclusion

We conclude that pellicles from adults and children promote different erosion protective effects, where adult pellicle provides better protection for permanent enamel, and child pellicle promotes better protection on deciduous enamel.

Clinical relevance

The present results provide a better understanding toward the protective effect of salivary pellicle against dental erosion and brings light to one more factor involved in the erosion of deciduous teeth.

Shellis RP, Barbour ME, Jesani A, Lussi A (2013) Effects of buffering properties and undissociated acid concentration on dissolution of dental enamel in relation to pH and acid type. Caries Res 47:601–611. doi:10.1159/000351641 CrossRefPubMed

Schlueter N, Hara A, Shellis RP, Ganss C (2011) Methods for the measurement and characterization of erosion in enamel and dentine. Caries Res 45(Suppl 1):13–23. doi:10.1159/000326819 CrossRefPubMed

Attin T, Wegehaupt F (2014) Methods for assessment of dental erosion. Monogr Oral Sci 25:123–142. doi:10.1159/000360355 CrossRefPubMed

Rakhmatullina E, Bossen A, Höschele C, Wang X, Beyeler B, Meier C, Lussi A (2011) Application of the specular and diffuse reflection analysis for in vitro diagnostics of dental erosion: correlation with enamel softening, roughness, and calcium release. J Biomed Opt 16:107002. doi:10.1117/1.3631791 CrossRefPubMedPubMedCentral

Lussi A, Bossen A, Höschele C, Beyeler B, Megert B, Meier C, Rakhmatullina E (2012) Effects of enamel abrasion, salivary pellicle, and measurement angle on the optical assessment of dental erosion. J Biomed Opt 17:97009–1. doi:10.1117/1.JBO.17.9.097009 CrossRefPubMed

Brevik SC, Lussi A, Rakhmatullina E (2013) A new optical detection method to assess the erosion inhibition by in vitro salivary pellicle layer. J Dent 41:428–435. doi:10.1016/j.jdent.2013.02.011 CrossRefPubMed

Hannig C, Hannig M, Attin T (2005) Enzymes in the acquired enamel pellicle. Eur J Oral Sci 113:2–13. doi:10.1111/j.1600-0722.2004.00180.x CrossRefPubMed

Zimmerman JN, Custodio W, Hatibovic-Kofman S, Lee YH, Xiao Y, Siqueira WL (2013) Proteome and peptidome of human acquired enamel pellicle on deciduous teeth. Int J Mol Sci 14:920–934. doi:10.3390/ijms14010920 CrossRefPubMedPubMedCentral

Hannig M, Hannig C (2014) The pellicle and erosion. Monogr Oral Sci 25:206–214. doi:10.1159/000360376 CrossRefPubMed

10.

Vukosavljevic D, Custodio W, Buzalaf MA, Hara AT, Siqueira WL (2014) Acquired pellicle as a modulator for dental erosion. Arch Oral Biol 59:631–638. doi:10.1016/j.archoralbio.2014.02.002 CrossRefPubMed

11.

Cabras T, Pisano E, Boi R, Olianas A, Manconi B, Inzitari R, Fanali C, Giardina B, Castagnola M, Messana I (2009) Age-dependent modifications of the human salivary secretory protein complex. J Proteome Res 8:4126–4134. doi:10.1021/pr900212u CrossRefPubMed

12.

Ben-Aryeh H, Fisher M, Szargel R, Laufer D (1990) Composition of whole unstimulated saliva of healthy children: changes with age. Arch Oral Biol 35:929–931CrossRefPubMed

13.

Messana I, Cabras T, Iavarone F, Manconi B, Huang L, Martelli C, Olianas A, Sanna MT, Pisano E, Sanna M, Arba M, D’Alessandro A, Desiderio C, Vitali A, Pirolli D, Tirone C, Lio A, Vento G, Romagnoli C, Cordaro M, Manni A, Gallenzi P, Fiorita A, Scarano E, Calo L, Passali GC, Picciotti PM, Paludetti G, Fanos V, Faa G, Castagnola M (2015) Chrono-proteomics of human saliva: variations of the salivary proteome during human development. J Proteome Res 14:1666–1677. doi:10.1021/pr501270x CrossRefPubMed

14.

Ruhl S, Rayment SA, Schmalz G, Hiller KA, Troxler RF (2005) Proteins in whole saliva during the first year of infancy. J Dent Res 84:29–34CrossRefPubMed

15.

Sønju Clasen AB, Hannig M, Skjørland K, Sønju T (1997) Analytical and ultrastructural studies of pellicle on primary teeth. Acta Odontol Scand 55:339–343PubMed

16.

Zero DT, Rahbek I, Fu J, Proskin HM, Featherstone JD (1990) Comparison of the iodide permeability test, the surface microhardness test, and mineral dissolution of bovine enamel following acid challenge. Caries Res 24:181–188CrossRefPubMed

17.

Rakhmatullina E, Bossen A, Bachofner KK, Meier C, Lussi A (2013) Optical pen-size reflectometer for monitoring of early dental erosion in native and polished enamels. J Biomed Opt 18:117009. doi:10.1117/1.JBO.18.11.117009 CrossRefPubMed

18.

Brunner E, Domhof S, Langer F (2002) Nonparametric analysis of longitudinal data in factorial experiments. Book title, 1st edn. Wiley, New York

19.

Meurman JH, Frank RM (1991) Scanning electron microscopic study of the effect of salivary pellicle on enamel erosion. Caries Res 25:1–6CrossRefPubMed

20.

Hara AT, Zero DT (2014) The potential of saliva in protecting against dental erosion. Monogr Oral Sci 25:197–205. doi:10.1159/000360372 CrossRefPubMed

21.

Carvalho TS, Lussi A, Jaeggi T, Gambon DL (2014) Erosive tooth wear in children. Monogr Oral Sci 25:262–278. doi:10.1159/000360712 CrossRefPubMed

22.

Martins C, Castro GF, Siqueira MF, Xiao Y, Yamaguti PM, Siqueira WL (2013) Effect of dialyzed saliva on human enamel demineralization. Caries Res 47:56–62. doi:10.1159/000343574 CrossRefPubMed

23.

Anderson P, Hector MP, Rampersad MA (2001) Critical pH in resting and stimulated whole saliva in groups of children and adults. Int J Paediatr Dent 11:266–273CrossRefPubMed

24.

Yao Y, Berg EA, Costello CE, Troxler RF, Oppenheim FG (2003) Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches. J Biol Chem 278:5300–5308. doi:10.1074/jbc.M206333200 CrossRefPubMed

25.

Siqueira WL, Zhang W, Helmerhorst EJ, Gygi SP, Oppenheim FG (2007) Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS. J Proteome Res 6:2152–2160. doi:10.1021/pr060580k CrossRefPubMed

26.

Ganss C, Klimek J, Schwarz N (2000) A comparative profilometric in vitro study of the susceptibility of polished and natural human enamel and dentine surfaces to erosive demineralization. Arch Oral Biol 45:897–902CrossRefPubMed

27.

Robinson C, Weatherell JA, Hallsworth AS (1971) Variation in composition of dental enamel within thin ground tooth sections. Caries Res 5:44–57CrossRefPubMed

28.

Weatherell JA, Robinson C, Hallsworth AS (1974) Variations in the chemical composition of human enamel. J Dent Res 53:180–192CrossRefPubMed

29.

Carvalho TS, Lussi A (2015) Susceptibility of enamel to initial erosion in relation to tooth type, tooth surface and enamel depth. Caries Res 49:109–115. doi:10.1159/000369104 CrossRefPubMed

30.

Wallwork ML, Kirkham J, Zhang J, Smith DA, Brookes SJ, Shore RC, Wood SR, Ryu O, Robinson C (2001) Binding of matrix proteins to developing enamel crystals: an atomic force microscopy study. Langmuir 17:2508–2513. doi:10.1021/La001281r CrossRef

31.

Sønju Clasen AB, Ruyter IE (1997) Quantitative determination of type A and type B carbonate in human deciduous and permanent enamel by means of Fourier transform infrared spectrometry. Adv Dent Res 11:523–527CrossRefPubMed

Title: In vitro salivary pellicles from adults and children have different protective effects against erosion
Authors: Thiago S. Carvalho
Tommy Baumann
Adrian Lussi
Publication date: 01-11-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 8/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1703-1

At a glance: The STEP trials

Springer Medicine

In vitro salivary pellicles from adults and children have different protective effects against erosion

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Please log in to get access to this content

Other articles of this Issue 8/2016

Involvement of oral tissues by AL amyloidosis: a literature review and report of eight new cases

Lactate as a predictive marker for tumor recurrence in patients with head and neck squamous cell carcinoma (HNSCC) post radiation: a prospective study over 15 years

Osteoinductive potential of 4 commonly employed bone grafts

An oral health survey of vulnerable older people in Belgium

Patient’s pain perception during mandibular molar extraction with articaine: a comparison study between infiltration and inferior alveolar nerve block

Erratum to: Significance of endothelial progenitor cells (EPC) for tumorigenesis of head and neck squamous cell carcinoma (HNSCC): possible marker of tumor progression and neovascularization?