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Clinical Oral Investigations

Published in:

01-03-2015 | Original Article

In vitro and ex vivo antimicrobial efficacy of nano-MgO in the elimination of endodontic pathogens

Authors: Abbas Monzavi, Saeed Eshraghi, Roxana Hashemian, Fatemeh Momen-Heravi

Published in: Clinical Oral Investigations | Issue 2/2015

Abstract

Objectives

The use of metal oxide nanoparticles has attracted lots of attention, mostly because of their promising antimicrobial activity along with their biocompatibility with mammalian cells. This study aims to investigate the in vitro and ex vivo antimicrobial efficiency of nano-magnesium oxide (MgO) aqueous solution against endodontic pathogens.

Materials and methods

The cytotoxicity of different concentrations of nano-MgO was assessed using lactate dehydrogenase cytotoxicity assay (LDH assay). A comparison of the antimicrobial efficiency of several concentrations of nano-MgO solution, sodium hypochlorite (NaOCl), and chlorhexidine (CHX) gluconate against Staphylococcus aureus, Enterococcus faecalis, and Candida albicans was made using the direct contact method. An ex vivo model of decoronated and experimentally infected human teeth was employed to compare the efficiency of nano-MgO (5 mg/L) solution with NaOCl (5.25 %) in the elimination of E. faecalis.

Results

There was no statistically significant difference between nano-MgO solutions (10 and 5 mg/L), 5.25 % NaOCl, and 2 % CHX gluconate in terms of the required time to inhibit the growth of the tested pathogens (p > 0.05). The LDH assay showed no cytotoxicity of different concentrations of nano-MgO used in this study (p < 0.001). In the ex vivo model of infected human teeth, 6 h post-irrigation, there was no statistically significant difference between colony-forming units (CFU) per milliliter of nano-MgO (5 mg/L) and NaOCl (5.25 %)-treated teeth (5–6 log scale reduction). However, the nano-MgO group showed a significant decrease in colony-forming units per milliliter (7 log scale), 24 h post-irrigation (p < 0.05). At other tested time points—24, 48, 72, and 168 h—the levels of CFU per milliliter were significantly less in the nano-MgO group (2–3 log scale difference) compared to the NaOCl group, indicating long-term antibacterial activity of nano-MgO (p < 0.05). At 72 and 168 h post-irrigation, no detectable bacterial growth was observed in the nano-MgO group. The detection limit was 10 CFU/mL.

Conclusions

Nano-MgO aqueous solutions represent promising antimicrobial activities, both in vitro and ex vivo with minimal toxicity.

Clinical relevance

Compared to NaOCl (5.25 %), nano-MgO (5 mg/L) exhibits statistically significant long-term efficiency in the elimination of E. faecalis in the root canal system. After further investigations, nano-MgO could be considered as a new root canal irrigant.

Câmara AC, de Albuquerque MM, Aguiar CM (2009) de Barros Correia AC. In vitro antimicrobial activity of 0.5 %, 1 %, and 2.5 % sodium hypochlorite in root canal instruments with the ProTaper Universal system. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:e55–e56CrossRefPubMed

Radcliffe CE, Potouridou L, Qureshi R, Habahbeh N, Qualtrough A, Worthington H et al (2004) Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms Actinomyces israelii, A. naeslundii, Candida albicans and Enterococcus faecalis. Int Endod J 37:438–446CrossRefPubMed

Stuart CH, Schwartz SA, Beeson TJ, Owatz CB (2006) Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 32:93–98CrossRefPubMed

Sunde PT, Olsen I, Debelian GJ, Tronstad L (2002) Microbiota of periapical lesions refractory to endodontic therapy. J Endod 28:304–310CrossRefPubMed

Neglia R, Ardizzoni A, Giardino L, Ambu E, Grazi S, Calignano S, Rimoldi C, Righi E, Blasi E (2008) Comparative in vitro and ex vivo studies on the bactericidal activity of Tetraclean, a new generation endodontic irrigant, and sodium hypochlorite. New Microbiol 31:57–65PubMed

Retamozo B, Shabahang S, Johnson N, Aprecio RM, Torabinejad M (2010) Minimum contact time and concentration of sodium hypochlorite required to eliminate Enterococcus faecalis. J Endod 36:520–523CrossRefPubMed

Lebreton F, Riboulet-Bisson E, Serror P, Sanguinetti M, Posteraro B, Torelli R et al (2009) Ace, which encodes an adhesin in Enterococcus faecalis, is regulated by Ers and is involved in virulence. Infect Immun 77:2832–2839CrossRefPubMedCentralPubMed

Sakamoto M, Siqueira JF Jr, Rôças IN, Benno Y (2008) Molecular analysis of the root canal microbiota associated with endodontic treatment failures. Oral Microbiol Immunol 23:275–278CrossRefPubMed

Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M (2000) Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod 26:593–595CrossRefPubMed

10.

Blount CA, Leser C (2012) Multisystem complications following endodontic therapy. J Oral Maxillofac Surg 70:527–530CrossRefPubMed

11.

Sundqvist G, Figdor D, Persson S, Sjogren U (1998) Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:86–93CrossRefPubMed

12.

Estrela C, Silva JA, de Alencar AH, Leles CR, Decurcio DA et al (2008) Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis—a systematic review. J Appl Oral Sci 16:364–368CrossRefPubMed

13.

Zehnder M (2006) Root canal irrigants. J Endod 32:389–398CrossRefPubMed

14.

Giardino L, Ambu E, Becce C, Rimondini L, Morra M (2006) Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. J Endod 32:1091–1093CrossRefPubMed

15.

Arias-Moliz MT, Ferrer-Luque CM, González-Rodríguez MP, Valderrama MJ, Baca P (2010) Eradication of Enterococcus faecalis biofilms by cetrimide and chlorhexidine. J Endod 36:87–90CrossRefPubMed

16.

Shih M, Marshall FJ, Rosen S (1970) The bactericidal efficiency of sodium hypochlorite as an endodontic irrigant. Oral Surg Oral Med Oral Pathol 29:613–619CrossRefPubMed

17.

Ardizzoni A, Blasi E, Rimoldi C, Giardino L, Ambu E, Righi E, Neglia R (2009) An in vitro and ex vivo study on two antibiotic-based endodontic irrigants: a challenge to sodium hypochlorite. New Microbiol 32:57–66PubMed

18.

19.

Gomes BPFA, Drucker DB, Lilley JD (1996) Association of endodontic symptoms and signs with particular combinations of specific bacteria. Int Endod J 29:69–75CrossRefPubMed

20.

Cheung GSP, Stock CJR (1993) In vitro cleaning ability of root canal irrigants with and without endodontics. Int Endod J 26:334–343CrossRefPubMed

21.

Anagnostakos K, Hitzler P, Pape D, Kohn D, Kelm J (2008) Persistence of bacterial growth on antibiotic-loaded beads: is it actually a problem? Acta Orthop 79:302–307CrossRefPubMed

22.

Huang L, Li DQ, Lin YJ, Wei M, Evans DG, Duan X (2005) Controllable preparation of Nano-MgO and investigation of its bactericidal properties. J Inorg Biochem 9:986–993CrossRef

23.

Jin T, He Y (2011) Antibacterial activities of magnesium oxide (MgO) nanoparticles against foodborne pathogens. J Nanoparticle Res 13:6877–688CrossRef

24.

Leung YH, Ng AM, Xu X, Shen Z, Gethings LA, Wong MT et al (2013) Mechanisms of antibacterial activity of MgO: non-ROS mediated toxicity of MgO nanoparticles towards Escherichia coli. Small. doi:10.1002/smll.201302434 PubMed

25.

Vidic J, Stankic S, Haque F, Ciric D, Le Goffic R, Vidy A (2013) Selective antibacterial effects of mixed ZnMgO nanoparticles. J Nanoparticle Res 15:1595CrossRef

26.

Amal R, Coury JR, Raper JA, Walsh WP, Waite TD (1990) Structure and kinetics of aggregating colloidal hematite. Colloids Surf 46:1–19CrossRef

27.

Javidi M, Afkhami F, Zarei M, Ghazvini K, Rajabi O (2013) Efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of Enterococcus faecalis. Aust Endod J. doi:10.1111/aej.12028 PubMed

28.

Onçağ O, Hoşgör M, Hilmioğlu S, Zekioğlu O, Eronat C, Burhanoğlu D (2003) Comparison of antibacterial and toxic effects of various root canal irrigants. Int Endod J 36:423–432CrossRefPubMed

29.

Shabahang S, Torabinejad M (2003) Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth. J Endod 29:576–579CrossRefPubMed

30.

Lin YJ, Li DQ, Wang G, Huang L, Duan X (2005) Preparation and bactericidal property of MgO nanoparticles on gamma-Al₂O₃. J Mater Sci Mater Med 16:53–56CrossRefPubMed

31.

Zhang K, An Y, Zhang L, Dong Q (2012) Preparation of controlled nano-MgO and investigation of its bactericidal properties. Chemosphere 89:1414–1418CrossRefPubMed

32.

Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J (2010) Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod Toxicol 30:343–345CrossRefPubMed

33.

Koper OB, Klabunde JS, Marchin GL, Klabunde KJ, Stoimenov P, Bohra L (2002) Nanoscale powders and formulations with biocidal activity toward spores and vegetative cells of bacillus species, viruses, and toxins. Curr Microbiol 44:49–55CrossRefPubMed

34.

Berry V, Gole A, Kundu S, Murphy CJ, Saraf RF (2005) Deposition of CTAB-terminated nanorods on bacteria to form highly conducting hybrid systems. J Am Chem Soc 127:17600–17601CrossRefPubMed

35.

Yamamoto O, Sawai J, Sasamoto T (2000) Change in antibacterial characteristics with doping amount of ZnO in MgO–ZnO solid solution. Int J Inorg Mater 2:451–455CrossRef

36.

Stankic S, Sternig A, Finocchi F, Bernardi J, Diwald O (2010) Zinc oxide scaffolds on MgO nanocubes. Nanotechnology 21:355603CrossRefPubMed

37.

Vidic J, Stankic S, Haque F, Ciric D, Le Goffic R, Vidy A et al (2013) Selective antibacterial effects of mixed ZnMgO nanoparticles. J Nanoparticle Res 15:1595CrossRef

38.

Jahangiri L, Kesmati M, Najafzadeh H (2013) Evaluation of analgesic and anti-inflammatory effect of nanoparticles of magnesium oxide in mice with and without ketamine. Eur Rev Med Pharmacol Sci 17:2706–2710PubMed

39.

Joshi S, Ghosh I, Pokhrel S, Mädler L, Nau WM (2012) Interactions of amino acids and polypeptides with metal oxide nanoparticles probed by fluorescent indicator adsorption and displacement. ACS Nano 6:5668–5679CrossRefPubMed

40.

Bouwmeester H, Dekkers S, Noordam MY, Hagens WI, Bulder AS, de Heer C et al (2009) Review of health safety aspects of nanotechnologies in food production. Regul Toxicol Pharmacol 53:52–62CrossRefPubMed

41.

Magnuson BA, Jonaitis TS, Card JW (2011) A brief review of the occurrence, use, and safety of food-related nanomaterials. J Food Sci 76:R126–R133CrossRefPubMed

Title: In vitro and ex vivo antimicrobial efficacy of nano-MgO in the elimination of endodontic pathogens
Authors: Abbas Monzavi
Saeed Eshraghi
Roxana Hashemian
Fatemeh Momen-Heravi
Publication date: 01-03-2015
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1253-y

At a glance: The STEP trials

Springer Medicine

In vitro and ex vivo antimicrobial efficacy of nano-MgO in the elimination of endodontic pathogens

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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