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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2015

01-03-2015 | Original Article

Detection of herpesviruses and human papillomavirus in acute apical abscesses by real-time PCR

Authors: Ahmet Ozbek, Selcuk M. Ozbek

Published in: Clinical Oral Investigations | Issue 2/2015

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Abstract

Objective

The recent use of polymerase chain reaction (PCR)-based molecular methods to detect herpesviruses in periradicular lesions has suggested that some herpesviruses, especially human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV) can participate in the pathogenesis of the periradicular lesions. Based on the applicability of molecular techniques for virus identification, the aim of the study was to investigate the presence of HCMV, EBV, and human papillomavirus (HPV) in samples from acute apical abscesses. To the best of our knowledge, this is the first study in Turkish population to survey apical abscess samples for the presence of herpesviruses and HPV.

Material and methods

Microbial samples were collected from 27 apical abscess specimens and 6 control samples. DNA was extracted from clinical samples by using spin column-based nucleic acid purification method, and viral loads were evaluated using real-time PCR methods following the kit protocols recommended by the manufacturers.

Results

HCMV DNA was found alone in five (18.5 %) samples, EBV DNA alone in two (7.4 %), HPV DNA alone in two (7.4 %), and in one, both HCMV and EBV (4 %). As for the healthy pulps used as noninflamed controls, no control specimens contained HCMV, EBV, or HPV DNA.

Conclusion

The present PCR-based findings have identified HCMV, EBV, and HPV as an inhabitant of acute apical abscesses. Additional studies using in vitro or animal model systems are required to elucidate the role of HCMV, EBV, and HPV in the pathogenesis of periapical pathosis.

Clinical relevance

The present viral findings may have future therapeutic relevance for periapical abscesses and other periapical pathosis.
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Metadata
Title
Detection of herpesviruses and human papillomavirus in acute apical abscesses by real-time PCR
Authors
Ahmet Ozbek
Selcuk M. Ozbek
Publication date
01-03-2015
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 2/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-014-1251-0

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