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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2015

01-03-2015 | Original Article

Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing

Authors: H. Dommisch, J. Winter, W. Götz, J. Miesen, A. Klein, L. Hierse, J. Deschner, A. Jäger, J. Eberhard, S. Jepsen

Published in: Clinical Oral Investigations | Issue 2/2015

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Abstract

Background

Antimicrobial peptides (AMPs), such as human beta-defensin-2 (hBD-2) and the CC-chemokine ligand 20 (CCL20), exhibit direct microbicidal effects and mediator-like activity. It was hypothesized that wounding induces the expression of AMPs and pro-inflammatory mediators and that endogenous mediators, such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-alpha (TGF-alpha), modulate this induced expression.

Material and methods

Monolayers of gingival epithelial cells (GECs) and gingival fibroblast (HGFs) from three different donors were wounded using the scratch assay (in vitro wounding) in the presence (test group) or absence (control group) of IGF-1 and TGF-alpha. In vitro wound closure was monitored over time (0, 6, 24, 48, 72 h), and wound areas were microscopically analyzed (Axio-Vision® Software, Zeiss). Gene expression analysis of the GAPDH, hBD-2, CCL20, interleukin-1 beta (IL-1 beta), and interleukin-8 (IL-8) was performed by qPCR.

Results

In comparison to control cells, IGF-1 and TGF-alpha significantly enhanced in vitro wound closure (P < 0.05). In GECs, IGF-1 induced the gene expression of IL-1 beta and IL-8 when compared to control cells (P < 0.05). In HGFs, wounding per se induced the messenger RNA of hBD-2, CCL20, and IL-1 beta, whereas IGF-1 and TGF-alpha reversed this effect (P < 0.05).

Conclusion

In gingival cells, the gene expression of AMPs was altered by injury, and endogenous growth factors further influenced the expression profiles, but with high interindividual differences.
Appendix
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Metadata
Title
Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing
Authors
H. Dommisch
J. Winter
W. Götz
J. Miesen
A. Klein
L. Hierse
J. Deschner
A. Jäger
J. Eberhard
S. Jepsen
Publication date
01-03-2015
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 2/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-014-1239-9

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