Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Clinical Oral Investigations
Published in: Clinical Oral Investigations 1/2014

01-01-2014 | Original Article

Correlation of MAGE-A tumor antigens and the efficacy of various chemotherapeutic agents in head and neck carcinoma cells

Authors: S. Hartmann, U. Kriegebaum, N. Küchler, R. C. Brands, C. Linz, A. C. Kübler, U. D. A. Müller-Richter

Published in: Clinical Oral Investigations | Issue 1/2014

Login to get access

Abstract

Objectives

The present study examined the relationship between MAGE-A tumor antigens and the efficacy of diamindichloridoplatin (DDP), 5-fluorouracil (5-FU), docetaxel, and paclitaxel for in vitro treatment of head and neck cancer.

Methods

In the present study, five cell lines of human squamous cell carcinomas were treated with DDP (25–400 μM), 5-FU (0.75–12 mM), docetaxel (1.56–25 nM), and paclitaxel (1.56–25 nM) for a period of 24 or 48 h. The efficacy of the agents was observed dynamically using real-time cell analysis. Subsequently, the expression levels of MAGE-A1, MAGE-A5, MAGE-A8, MAGE-A9, MAGE-A11, and MAGE-A12 were determined by quantitative real-time polymerase chain reaction. Chemosensitivity and MAGE-A-expression were correlated by linear regression.

Results

The tumor cell lines showed a highly differentiated response to the chemotherapeutic agents. Expression of MAGE-A11 was significantly associated with a poorer response to treatment with DDP, 5-FU, docetaxel, and paclitaxel. Two cell lines, one of which was MAGE-A11-positive, showed a significant and concentration-dependent cisplatin-induced growth spurt during the first 24 h after treatment. MAGE-A5 was connected to a positive effect on treatment with paclitaxel within the first 24 h after application. In association with docetaxel treatment, MAGE-A8 was connected to a poorer susceptibility.

Conclusions

The results describe, for the first time, a correlation between these MAGE-A tumor antigens and the susceptibility of head and neck cancer cells to DDP, 5-FU, docetaxel, and paclitaxel.

Clinical relevance

These findings could affect the antineoplastic treatment of patients with MAGE-A11-positive tumors.
Literature
1.
van der Bruggen P et al (1991) A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. Science 254(5038):1643–1647PubMedCrossRef
2.
De Plaen E et al (1994) Structure, chromosomal localization, and expression of 12 genes of the MAGE family. Immunogenetics 40(5):360–369PubMedCrossRef
3.
Chomez P et al (2001) An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res 61(14):5544–5551PubMed
4.
Barker PA, Salehi A (2002) The MAGE proteins: emerging roles in cell cycle progression, apoptosis, and neurogenetic disease. J Neurosci Res 67(6):705–712PubMedCrossRef
5.
Muller-Richter UD et al (2008) Different expression of MAGE-A-antigens in foetal and adult keratinocyte cell lines. Oral Oncol 44(7):628–633PubMedCrossRef
6.
Yang B et al (2007) MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines. Cancer Res 67(20):9954–9962PubMedCrossRef
7.
Lian Y et al (2012) Expressions of MAGE-A10 and MAGE-A11 in breast cancers and their prognostic significance: a retrospective clinical study. J Cancer Res Clin Oncol 138(3):519–527PubMedCrossRef
8.
Qiu G, Fang J, He Y (2006) 5′ CpG island methylation analysis identifies the MAGE-A1 and MAGE-A3 genes as potential markers of HCC. Clin Biochem 39(3):259–266PubMedCrossRef
9.
Muller-Richter UD et al (2010) MAGE-A antigens in patients with primary oral squamous cell carcinoma. Clin Oral Investig 14(3):291–296PubMedCrossRef
10.
Hussein YM et al (2011) The melanoma-associated antigen-A3, -A4 genes: relation to the risk and clinicopathological parameters in breast cancer patients. Mol Cell Biochem 351(1–2):261–268PubMedCrossRef
11.
Nardiello T et al (2011) MAGE-A inhibits apoptosis in proliferating myeloma cells through repression of Bax and maintenance of survivin. Clin Cancer Res 17(13):4309–4319PubMedCentralPubMedCrossRef
12.
Ries J et al (2005) Investigation of the expression of melanoma antigen-encoding genes (MAGE-A1 to -A6) in oral squamous cell carcinomas to determine potential targets for gene-based cancer immunotherapy. Int J Oncol 26(3):817–824PubMed
13.
Krauss E et al (2011) MAGE-A antigens in lesions of the oral mucosa. Clin Oral Investig 15(3):315–320PubMedCrossRef
14.
Mollaoglu N et al (2011) Prediction of recurrence using exfoliative cytology and melanoma-associated antigen-A mRNA analysis following wide excision of oral squamous cell carcinoma: short report. Cytopathology 22(6):387–391PubMedCrossRef
15.
Muller-Richter UDA et al (2010) Impact of MAGE-A antigens on taxane response in oral squamous cell carcinoma. Oncol Lett 1(1):181–185PubMedCentralPubMed
16.
Heo DS et al (1989) Biology, cytogenetics, and sensitivity to immunological effector cells of new head and neck squamous cell carcinoma lines. Cancer Res 49(18):5167–5175PubMed
17.
18.
Dowejko A et al (2012) The human HECA interacts with cyclins and CDKs to antagonize Wnt-mediated proliferation and chemoresistance of head and neck cancer cells. Exp Cell Res 318(5):489–499PubMedCrossRef
19.
Dowejko A et al (2009) The human homolog of the Drosophila headcase protein slows down cell division of head and neck cancer cells. Carcinogenesis 30(10):1678–1685PubMedCrossRef
20.
Arias LR, Perry CA, Yang L (2010) Real-time electrical impedance detection of cellular activities of oral cancer cells. Biosens Bioelectron 25(10):2225–2231PubMedCrossRef
21.
22.
Monte M et al (2006) MAGE-A tumor antigens target p53 transactivation function through histone deacetylase recruitment and confer resistance to chemotherapeutic agents. Proc Natl Acad Sci U S A 103(30):11160–11165PubMedCentralPubMedCrossRef
23.
Doyle JM et al (2010) MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. Mol Cell 39(6):963–974PubMedCrossRef
24.
25.
Liu W et al (2008) The melanoma-associated antigen A3 mediates fibronectin-controlled cancer progression and metastasis. Cancer Res 68(19):8104–8112PubMedCrossRef
26.
Perera FP (1996) Molecular epidemiology: insights into cancer susceptibility, risk assessment, and prevention. J Natl Cancer Inst 88(8):496–509PubMedCrossRef
27.
28.
Tsai JR et al (2007) Differential expression profile of MAGE family in non-small-cell lung cancer. Lung Cancer 56(2):185–192PubMedCrossRef
29.
Camisasca DR et al (2011) Oral squamous cell carcinoma: clinicopathological features in patients with and without recurrence. ORL J Otorhinolaryngol Relat Spec 73(3):170–176PubMedCrossRef
30.
Simpson AJ et al (2005) Cancer/testis antigens, gametogenesis and cancer. Nat Rev Cancer 5(8):615–625PubMedCrossRef
Metadata
Title
Correlation of MAGE-A tumor antigens and the efficacy of various chemotherapeutic agents in head and neck carcinoma cells
Authors
S. Hartmann
U. Kriegebaum
N. Küchler
R. C. Brands
C. Linz
A. C. Kübler
U. D. A. Müller-Richter
Publication date
01-01-2014
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 1/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-013-0936-0

Other articles of this Issue 1/2014

Clinical Oral Investigations 1/2014 Go to the issue

Original Article

Tissue engineering strategies for alveolar cleft reconstruction: a systematic review of the literature

Original Article

Does an intracanal composite anchorage replace posts?

Original Article

Desmoglein-3/γ-catenin and E-cadherin/ß-catenin differential expression in oral leukoplakia and squamous cell carcinoma

Original Article

Oral and genital HPV genotypic concordance between sexual partners

Original Article

Effect of modulated irradiation time on the degree of conversion and the amount of elutable substances from nano-hybrid resin-based composites

Original Article

Regulation of visfatin by microbial and biomechanical signals in PDL cells