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Clinical Oral Investigations

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01-04-2013 | Original Article

Histological changes and changes in the myosin mRNA content of the porcine masticatory muscles after masseter treatment with botulinum toxin A

Authors: T. Gedrange, T. Gredes, A. Spassov, R. Mai, D. U. Kuhn, M. Dominiak, C. Kunert-Keil

Published in: Clinical Oral Investigations | Issue 3/2013

Abstract

Objectives

Botulinum toxin A (Botox) is increasingly used for treatment of muscle hyperfunction. For a better understanding of the possible morphologic and chewing changes in patients induced by a therapy with Botox, muscle fiber and myosin heavy chain (MyHC) mRNA alterations were examined in this animal study.

Materials and methods

The investigation was carried out on 14-week-old pigs (seven treated animals, eight controls; calculated animal size with a power of 0.5). To initialise the total immobilisation of the right masseter, the Botox injection was distributed into ten areas. After a 56-day period, muscle tissue was taken from the left and right side of the masseter (three regions), temporal (two regions), medial pterygoid and geniohyoid muscles using a standardized method. The muscle fiber cross sections were examined immunohistochemically. Fiber staining was accomplished with antibodies to specific MyHC isoforms. The MyHC mRNA changes were analysed using real-time RT-PCR.

Results

Muscles adapt to such stress by changing fiber types and MyHC mRNA content. Paralysed masseters display atrophic changes while other masticatory muscles show hypertrophic changes. The results indicated that the typical distributions of type IIa und IIb fiber types in masticatory muscles were increased in the masseter muscles due to Botox application. On the other hand, the masseters without Botox in the treated group showed a significant increase of type I MyHC.

Conclusions

Application of Botox may lead to uncontrolled structural changes in affected and unaffected muscles.

Clinical relevance

Treatment of muscle hypertrophy with Botox may cause muscle imbalance.

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Title: Histological changes and changes in the myosin mRNA content of the porcine masticatory muscles after masseter treatment with botulinum toxin A
Authors: T. Gedrange
T. Gredes
A. Spassov
R. Mai
D. U. Kuhn
M. Dominiak
C. Kunert-Keil
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 3/2013
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0750-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Histological changes and changes in the myosin mRNA content of the porcine masticatory muscles after masseter treatment with botulinum toxin A

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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