Skip to main content
Top
Published in: Clinical Oral Investigations 2/2013

01-03-2013 | Original Article

Disinfection effect of dental impression tray adhesives

Authors: Tobias Bensel, Rita Pollak, Michael Stimmelmayr, Jeremias Hey

Published in: Clinical Oral Investigations | Issue 2/2013

Login to get access

Abstract

Objectives

Iatrogenic infections are serious problems in dental offices. Impression tray adhesives are delivered in glass containers with a fixed brush attached inside the cap. Using the brush for application of the impression tray adhesive on a contaminated impression tray or prostheses, pathogen transmission by replacing the cap with the brush is possible.

Materials and methods

Bacterial strains (patient strains and in vitro strains) were supervaccinated on Columbia agar. The bacterial solution was diluted with TSB and aerobically grown, and starting concentration was 1 × 107 cfu/ml. The stock solution was placed on Columbia agar. Alginate, polyether, and silicon impression tray adhesives were applied to the center of the particular blood agar plates and incubated for 48 h. The expansion of the inhibition zone assays were measured using a microscope.

Results

Twenty-one different bacterial strains were selected in the saliva samples of 20 patients. The growth inhibition for alginate impression tray adhesive was 1.1 % (±0.3) of the patient strains. The overgrowth of polyether impression tray adhesive was 30.6 % (±9.3) and for silicon impression tray adhesive 11.8 % (±5.0). In in vitro strains, alginate impression tray adhesive performed an inhibition of 0.7 % (±0.3). The overgrowth of polyether impression tray adhesive was 7.0 % (±1.6) and for silicon impression tray adhesive was 6.5 % (±1.3).

Conclusions

Using the fixed brush for application of the impression tray adhesive on multiple patients, a cross-contamination cannot be ruled out.

Clinical relevance

An application of the impression tray adhesive with a pipette and a single-use brush would eliminate the contamination.
Literature
1.
go back to reference Rubino S, Cappuccinelli P, Kelvin DJ (2011) Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France. J Infect Dev Ctries 5:437–440PubMed Rubino S, Cappuccinelli P, Kelvin DJ (2011) Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France. J Infect Dev Ctries 5:437–440PubMed
2.
go back to reference Carlet J, Astagneau P, Brun-Buisson C, Coignard B, Salomon V, Tran B et al (2009) French national program for prevention of healthcare-associated infections and antimicrobial resistance, 1992–2008: positive trends, but perseverance needed. Infect Control Hosp Epidemiol 30:737–745PubMedCrossRef Carlet J, Astagneau P, Brun-Buisson C, Coignard B, Salomon V, Tran B et al (2009) French national program for prevention of healthcare-associated infections and antimicrobial resistance, 1992–2008: positive trends, but perseverance needed. Infect Control Hosp Epidemiol 30:737–745PubMedCrossRef
3.
go back to reference Cristina ML, Spagnolo AM, Sartini M, Dallera M, Ottria G, Perdelli F et al (2009) Investigation of organizational and hygiene features in dentistry: a pilot study. J Prev Med Hyg 50:175–180PubMed Cristina ML, Spagnolo AM, Sartini M, Dallera M, Ottria G, Perdelli F et al (2009) Investigation of organizational and hygiene features in dentistry: a pilot study. J Prev Med Hyg 50:175–180PubMed
4.
go back to reference Gyorfi A, Fazekas A (2007) Significance of infection control in dentistry: a review. Fogorv Sz 100:141–152PubMed Gyorfi A, Fazekas A (2007) Significance of infection control in dentistry: a review. Fogorv Sz 100:141–152PubMed
5.
go back to reference Szymanska J (2005) Microbiological risk factors in dentistry. Current status of knowledge. Ann Agric Environ Med 12:157–163PubMed Szymanska J (2005) Microbiological risk factors in dentistry. Current status of knowledge. Ann Agric Environ Med 12:157–163PubMed
6.
go back to reference Araujo MW, Andreana S (2002) Risk and prevention of transmission of infectious diseases in dentistry. Quintessence Int 33:376–382PubMed Araujo MW, Andreana S (2002) Risk and prevention of transmission of infectious diseases in dentistry. Quintessence Int 33:376–382PubMed
7.
go back to reference Abe S, Ishihara K, Okuda K (2001) Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care. Arch Gerontol Geriatr 32:45–55PubMedCrossRef Abe S, Ishihara K, Okuda K (2001) Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care. Arch Gerontol Geriatr 32:45–55PubMedCrossRef
8.
go back to reference Bergmann-Krauss B, Micheelis W, Szecsenyi J (2010) Acceptance of dental care guidelines by quality circles. Z Evid Fortbild Qual Gesundhwes 104:599–604PubMedCrossRef Bergmann-Krauss B, Micheelis W, Szecsenyi J (2010) Acceptance of dental care guidelines by quality circles. Z Evid Fortbild Qual Gesundhwes 104:599–604PubMedCrossRef
9.
go back to reference White JT, Jordan RD (1987) Infection control during elastomeric impressions. J Prosthet Dent 58:711–712PubMedCrossRef White JT, Jordan RD (1987) Infection control during elastomeric impressions. J Prosthet Dent 58:711–712PubMedCrossRef
10.
go back to reference Puttaiah R, Cederberg R, Youngblood D (2006) A pragmatic approach towards singleuse-disposable devices in dentistry. Bull Group Int Rech Sci Stomatol Odontol 47:18–26PubMed Puttaiah R, Cederberg R, Youngblood D (2006) A pragmatic approach towards singleuse-disposable devices in dentistry. Bull Group Int Rech Sci Stomatol Odontol 47:18–26PubMed
11.
go back to reference Herman DA (1993) A study of the antimicrobial properties of impression tray adhesives. J Prosthet Dent 69:102–105PubMedCrossRef Herman DA (1993) A study of the antimicrobial properties of impression tray adhesives. J Prosthet Dent 69:102–105PubMedCrossRef
12.
go back to reference Lewis T, Patel V, Ismail A, Fraise A (2009) Sterilisation, disinfection and cleaning of theatre equipment: do we need to extend the Spaulding classification? J Hosp Infect 72:361–363PubMedCrossRef Lewis T, Patel V, Ismail A, Fraise A (2009) Sterilisation, disinfection and cleaning of theatre equipment: do we need to extend the Spaulding classification? J Hosp Infect 72:361–363PubMedCrossRef
13.
go back to reference Kohn WG, Harte JA, Malvitz DM, Collins AS, Cleveland JL, Eklund KJ (2004) Guidelines for infection control in dental health care settings—2003. J Am Dent Assoc 135:33–47PubMed Kohn WG, Harte JA, Malvitz DM, Collins AS, Cleveland JL, Eklund KJ (2004) Guidelines for infection control in dental health care settings—2003. J Am Dent Assoc 135:33–47PubMed
14.
go back to reference Smith AJ, Bagg J, Hurrell D, McHugh S (2007) Sterilization of re-usable instruments in general dental practice. Br Dent J 203:E16PubMedCrossRef Smith AJ, Bagg J, Hurrell D, McHugh S (2007) Sterilization of re-usable instruments in general dental practice. Br Dent J 203:E16PubMedCrossRef
15.
go back to reference Chai JY, Jameson LM, Moser JB, Hesby RA (1991) Adhesive properties of several impression material systems: part II. J Prosthet Dent 66:287–292PubMedCrossRef Chai JY, Jameson LM, Moser JB, Hesby RA (1991) Adhesive properties of several impression material systems: part II. J Prosthet Dent 66:287–292PubMedCrossRef
16.
go back to reference Dethlefsen L, Fall-Ngai M, Relman DA (2007) An ecological and evolutionary perspective on human–microbe mutualism and disease. Nature 449:811–818PubMedCrossRef Dethlefsen L, Fall-Ngai M, Relman DA (2007) An ecological and evolutionary perspective on human–microbe mutualism and disease. Nature 449:811–818PubMedCrossRef
17.
go back to reference Zaura E, Keijser BJ, Huse SM, Crielaard W (2009) Defining the healthy “core microbiome” of oral microbial communities. BMC Microbiol 9:259PubMedCrossRef Zaura E, Keijser BJ, Huse SM, Crielaard W (2009) Defining the healthy “core microbiome” of oral microbial communities. BMC Microbiol 9:259PubMedCrossRef
18.
go back to reference Rozkiewicz D, Daniluk T, Zaremba ML, Cylwik-Rokicka D, Luczaj-Cepowicz E, Milewska R et al (2006) Bacterial composition in the supragingival plaques of children with and without dental caries. Adv Med Sci 51(Suppl 1):182–186PubMed Rozkiewicz D, Daniluk T, Zaremba ML, Cylwik-Rokicka D, Luczaj-Cepowicz E, Milewska R et al (2006) Bacterial composition in the supragingival plaques of children with and without dental caries. Adv Med Sci 51(Suppl 1):182–186PubMed
19.
go back to reference Sbordone L, Bortolaia C (2003) Oral microbial biofilms and plaque-related diseases: microbial communities and their role in the shift from oral health to disease. Clin Oral Investig 7:181–188PubMedCrossRef Sbordone L, Bortolaia C (2003) Oral microbial biofilms and plaque-related diseases: microbial communities and their role in the shift from oral health to disease. Clin Oral Investig 7:181–188PubMedCrossRef
20.
go back to reference Kuramitsu HK, He X, Lux R, Anderson MH, Shi W (2007) Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 71:653–670PubMedCrossRef Kuramitsu HK, He X, Lux R, Anderson MH, Shi W (2007) Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 71:653–670PubMedCrossRef
21.
go back to reference Nasidze I, Li J, Quinque D, Tang K, Stoneking M (2009) Global diversity in the human salivary microbiome. Genome Res 19:636–643PubMedCrossRef Nasidze I, Li J, Quinque D, Tang K, Stoneking M (2009) Global diversity in the human salivary microbiome. Genome Res 19:636–643PubMedCrossRef
Metadata
Title
Disinfection effect of dental impression tray adhesives
Authors
Tobias Bensel
Rita Pollak
Michael Stimmelmayr
Jeremias Hey
Publication date
01-03-2013
Publisher
Springer-Verlag
Published in
Clinical Oral Investigations / Issue 2/2013
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-012-0735-z

Other articles of this Issue 2/2013

Clinical Oral Investigations 2/2013 Go to the issue