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Clinical Oral Investigations

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01-12-2012 | Original Article

Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro

Authors: Safa Tuncer, Mustafa Demirci, Helmut Schweikl, Mine Erguven, Ayhan Bilir, Aysun Kara Tuncer

Published in: Clinical Oral Investigations | Issue 6/2012

Abstract

Objectives

The influence of dentin adhesive systems (Scotchbond Multi-Purpose, XP Bond, Xeno V, Clearfil Protect Bond, AdheSE) on cell survival, viability and proliferation was characterized after direct and indirect exposure using different cell culture techniques.

Materials and methods

The primers and cured bonding parts were directly exposed to cells using cell culture inserts, and complete materials were analyzed in a dentin barrier test. Cell responses were examined in 3T3 mouse fibroblasts after 24- and 72-h exposure periods by the estimation of total cell numbers (survival), apoptosis (viability) and cell proliferation.

Results

Cell numbers were effectively reduced by the primers of AdheSE, Protect Bond, and Scotchbond Multi-Purpose as well as XP bond after direct exposure in a cell culture insert test device. Likewise, Scotchbond Multi-Purpose primer induced a rate of apoptosis (93.9%) even higher than detected with Protect Bond primer (91.6%). Cell proliferation was entirely inhibited by primers and by Xp Bond as well. The Scotchbond Multi-Purpose was most cytotoxic in a dentin barrier test device after a 24-h indirect exposure. It also increased the percentage of cells in apoptosis to 15.4% compared to untreated controls.

Conclusion

Unpolymerized primers of dentin adhesives were more cytotoxic than polymerized bonding counterparts. Moreover, total etch dentin adhesives were more cytotoxic than self-etch adhesives.

Clinical relevance

When dentin adhesives are used in deep cavities without a protective dentin barrier the leachable hydrophobic and hydrophilic component of dentin adhesive systems can penetrate to the pulp and may induce cytotoxic responses in pulp tissues.

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Title: Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro
Authors: Safa Tuncer
Mustafa Demirci
Helmut Schweikl
Mine Erguven
Ayhan Bilir
Aysun Kara Tuncer
Publication date: 01-12-2012
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 6/2012
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-011-0669-x

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Springer Medicine

Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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