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01-06-2010 | Original Article

Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model

Authors: Isabelle Sliepen, Mark Van Essche, Marc Quirynen, Wim Teughels

Published in: Clinical Oral Investigations | Issue 3/2010

Abstract

The aim of the study was to evaluate the effects of Listerine®, Meridol®, and Perioaid® on the viability and total number of bacteria in established biofilms using an in vitro model under hydrodynamic conditions. Biofilms of Aggregatibacter actinomycetemcomitans were placed in a modified Robbins device and rinsed twice daily during 4 days. Bacteria were quantified by culture and quantitative polymerase chain reaction. Visualization of the samples was performed by scanning electron and confocal laser scanning microscopy, combined with a fluorescent vital staining. All three mouthrinses caused a significant reduction in the number of cultivable A. actinomycetemcomitans in a biofilm. Perioaid® was significantly the most powerful in killing the biofilm-protected bacteria and also in counteracting the development of thick dense microbial communities. The total amount of bacteria was not significantly affected by Listerine® and Meridol®.

Botelho MA, Bezerra Filho JG, Correa LL, Fonseca SG, Montenegro D, Gapski R, Brito GA, Heukelbach J (2007) Effect of a novel essential oil mouthrinse without alcohol on gingivitis: a double-blinded randomized controlled trial. J Appl Oral Sci 15:175–180CrossRefPubMed

Quirynen M, Papaioannou W, van Steenbergen TJ, Dierickx K, Cassiman JJ, van Steenberghe D (2001) Adhesion of Porphyromonas gingivalis strains to cultured epithelial cells from patients with a history of chronic adult periodontitis or from patients less susceptible to periodontitis. J Periodontol 72:626–633CrossRefPubMed

Silverman S, Wilder R (2006) Antimicrobial mouthrinse as part of a comprehensive oral care regimen. Safety and compliance factors. J Am Dent Assoc 137:22S–26SPubMed

Mandell RL, Socransky SS (1981) A selective medium for Actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis. J Periodontol 52:593–598PubMed

Slots J (1999) Update on Actinobacillus Actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease. J Int Acad Periodontol 1:121–126PubMed

Overholt BF (1966) Actinobacillus actinomycetemcomitans endocarditis. Arch Intern Med 117:99–102CrossRefPubMed

Muhle I, Rau J, Ruskin J (1979) Vertebral osteomyelitis due to Actinobacillus actinomycetemcomitans. JAMA 241:1824–1825CrossRefPubMed

Martin BF, Derby BM, Budzilovich GN, Ransohoff J (1967) Brain abscess due to Actinobacillus actinomycetemcomitans. Neurology 17:833–837PubMed

Kinniment SL, Wimpenny JW, Adams D, Marsh PD (1996) The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system. J Appl Bacteriol 81:120–125PubMed

10.

Haase EM, Bonstein T, Palmer RJ Jr, Scannapieco FA (2006) Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation. Arch Oral Biol 51:299–314CrossRefPubMed

11.

Ouhayoun JP (2003) Penetrating the plaque biofilm: impact of essential oil mouthwash. J Clin Periodontol 30(Suppl 5):10–12CrossRefPubMed

12.

Madlena M, Dombi C, Gintner Z, Banoczy J (2004) Effect of amine fluoride/stannous fluoride toothpaste and mouthrinse on dental plaque accumulation and gingival health. Oral Dis 10:294–297CrossRefPubMed

13.

Keijser JA, Verkade H, Timmerman MF, Van der Weijden FA (2003) Comparison of 2 commercially available chlorhexidine mouthrinses. J Periodontol 74:214–218CrossRefPubMed

14.

Wolff LF (1985) Chemotherapeutic agents in the prevention and treatment of periodontal disease. Northwest Dent 64:15–24PubMed

15.

Fine DH (1988) Mouthrinses as adjuncts for plaque and gingivitis management. A status report for the American Journal of Dentistry. Am J Dent 1:259–263PubMed

16.

Arweiler NB, Boehnke N, Sculean A, Hellwig E, Auschill TM (2006) Differences in efficacy of two commercial 0.2% chlorhexidine mouthrinse solutions: a 4-day plaque re-growth study. J Clin Periodontol 33:334–339CrossRefPubMed

17.

Busscher HJ, van der Mei HC (2006) Microbial adhesion in flow displacement systems. Clin Microbiol Rev 19:127–141CrossRefPubMed

18.

Boutaga K, van Winkelhoff AJ, Vandenbroucke-Grauls CM, Savelkoul PH (2005) Periodontal pathogens: a quantitative comparison of anaerobic culture and real-time PCR. FEMS Immunol Med Microbiol 45:191–199CrossRefPubMed

19.

Bretz F, Genz A, Hothorn LA (2001) On the numerical availability of multiple comparison procedures. Biom J 43:645–656CrossRef

20.

Bergmans L, Moisiadis P, Van Meerbeek B, Quirynen M, Lambrechts P (2005) Microscopic observation of bacteria: review highlighting the use of environmental SEM. Int Endod J 38:775–788CrossRefPubMed

21.

Cummins D, Creeth JE (1992) Delivery of antiplaque agents from dentifrices, gels, and mouthwashes. J Dent Res 71:1439–1449PubMed

22.

McClanahan SF, Bartizek RD (2002) Effects of triclosan/copolymer dentifrice on dental plaque and gingivitis in a 3-month randomized controlled clinical trial: influence of baseline gingivitis on observed efficacy. J Clin Dent 13:167–178PubMed

23.

Riep BG, Bernimoulin JP, Barnett ML (1999) Comparative antiplaque effectiveness of an essential oil and an amine fluoride/stannous fluoride mouthrinse. J Clin Periodontol 26:164–168CrossRefPubMed

24.

Arweiler NB, Netuschil L, Reich E (2001) Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study. J Clin Periodontol 28:168–174CrossRefPubMed

25.

Charles CH, Mostler KM, Bartels LL, Mankodi SM (2004) Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol 3:878–884CrossRef

26.

Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C (1990) Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol 17:575–579CrossRefPubMed

27.

Larsen T (2002) Susceptibility of Porphyromonas gingivalis in biofilms to amoxicillin, doxycycline and metronidazole. Oral Microbiol Immunol 17:267–271CrossRefPubMed

28.

Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322CrossRefPubMed

29.

Shapiro S, Giertsen E, Guggenheim B (2002) An in vitro oral biofilm model for comparing the efficacy of antimicrobial mouthrinses. Caries Res 36:93–100CrossRefPubMed

30.

Fine DH, Furgang D, Barnett ML (2001) Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of Actinobacillus actinomycetemcomitans. J Clin Periodontol 28:697–700CrossRefPubMed

31.

Larsen T, Fiehn NE (1995) Development of a flow method for susceptibility testing of oral biofilms in vitro. APMIS 103:339–344CrossRefPubMed

32.

Noiri Y, Okami Y, Narimatsu M, Takahashi Y, Kawahara T, Ebisu S (2003) Effects of chlorhexidine, minocycline, and metronidazole on Porphyromonas gingivalis strain 381 in biofilms. J Periodontol 74:1647–1651CrossRefPubMed

33.

Busscher HJ, Free RH, Van Weissenbruch R, Albers FW, van der Mei HC (2000) Preliminary observations on influence of dairy products on biofilm removal from silicone rubber voice prostheses in vitro. J Dairy Sci 83:641–647CrossRefPubMed

34.

Sliepen I, Van Essche M, Pauwels M, Van Eldere J, Hofkens J, Quirynen M, Teughels W (2008) Colonization of hard and soft surfaces by Aggregatibacter actinomycetemcomitans under hydrodynamic conditions. Oral Microbiol Immunol 23:498–504CrossRefPubMed

35.

Sliepen I, Hofkens J, Van Essche M, Quirynen M, Teughels W (2008) Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell. Oral Microbiol Immunol 23:520–524CrossRefPubMed

36.

Bercy P, Lasserre J (2007) Susceptibility to various oral antiseptics of Porphyromonas gingivalis W83 within a biofilm. Adv Ther 24:1181–1191CrossRefPubMed

37.

Paraskevas S (2005) Randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hyg 3:162–178CrossRefPubMed

38.

Van Strydonck DA, Timmerman MF, van der Weijden GA (2005) Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol 32:305–309CrossRefPubMed

39.

Brecx M, Netuschil L, Reichert B, Schreil G (1990) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses on plaque, gingivitis and plaque bacteria vitality. J Clin Periodontol 17:292–297CrossRefPubMed

40.

Netuschil L, Weiger R, Preisler R, Brecx M (1995) Plaque bacteria counts and vitality during chlorhexidine, Meridol and Listerine mouthrinses. Eur J Oral Sci 103:355–361CrossRefPubMed

41.

Brecx M, Brownstone E, MacDonald L, Gelskey S, Cheang M (1992) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses as supplements to regular tooth cleaning measures. J Clin Periodontol 19:202–207CrossRefPubMed

42.

Gunsolley JC (2006) A meta-analysis of six-month studies of antiplaque and antigingivitis agents. J Am Dent Assoc 137:1649–1657PubMed

43.

Stoeken JE, Paraskevas S, van der Weijden GA (2007) The long-term effect of a mouthrinse containing essential oils on dental plaque and gingivitis: a systematic review. J Periodontol 78:1218–1228CrossRefPubMed

44.

Fine DH, Furgang D, Schreiner HC, Goncharoff P, Charlesworth J, Ghazwan G, Fitzgerald-Bocarsly P, Figurski DH (1999) Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence. Microbiol 145:1335–1347CrossRef

45.

Fine DH, Kaplan JB, Kachlany SC, Schreiner HC (2006) How we got attached to Actinobacillus actinomycetemcomitans: a model for infectious diseases. Periodontol 2000(42):114–157CrossRef

46.

Zambon JJ, Slots J, Genco RJ (1983) Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. Infect Immun 41:19–27PubMed

47.

Okte E, Sultan N, Dogan B, Asikainen S (1999) Bacterial adhesion of Actinobacillus actinomycetemcomitans serotypes to titanium implants: SEM evaluation. A preliminary report. J Periodontol 70:1376–1382CrossRefPubMed

48.

Yang HW, Asikainen S, Dogan B, Suda R, Lai CH (2004) Relationship of Actinobacillus actinomycetemcomitans serotype b to aggressive periodontitis: frequency in pure cultured isolates. J Periodontol 75:592–599CrossRefPubMed

49.

Imbert-Bernard C, Valentin A, Reynes J, Mallie M, Bastide JM (1994) Relationship between fluconazole sensitivity of Candida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count. Eur J Clin Microbiol Infect Dis 13:711–716CrossRefPubMed

50.

Abbott JD, Graves JF (1972) Serotype and sulphonamide sensitivity of meningococci isolated from 1966 to 1971. J Clin Pathol 25:528–530CrossRefPubMed

51.

van der Mei HC, White DJ, Atema-Smit J, Belt-Gritter E, Busscher HJ (2006) A method to study sustained antimicrobial activity of rinse and dentifrice components on biofilm viability in vivo. J Clin Periodontol 33:14–20CrossRefPubMed

52.

Inoue T, Shingaki R, Sogawa N, Sogawa CA, Asaumi J, Kokeguchi S (2003) Biofilm formation by a fimbriae-deficient mutant of Actinobacillus actinomycetemcomitans. Microbiol Immunol 47:877–881PubMed

53.

Izano EA, Wang H, Ragunath C, Ramasubbu N, Kaplan JB (2007) Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS. J Dent Res 86:618–622CrossRefPubMed

54.

Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS (2002) Extracellular DNA required for bacterial biofilm formation. Science 295:1487CrossRefPubMed

55.

Fine DH, Markowitz K, Furgang D, Goldsmith D, Ricci-Nittel D, Charles CH, Peng P, Lynch MC (2007) Effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. J Periodontol 78:1935–1942CrossRefPubMed

Title: Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model
Authors: Isabelle Sliepen
Mark Van Essche
Marc Quirynen
Wim Teughels
Publication date: 01-06-2010
Publisher: Springer-Verlag
Published in: Clinical Oral Investigations / Issue 3/2010
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-009-0286-0

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Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model

Abstract

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