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01-11-2019 | Psychiatry and Preclinical Psychiatric Studies - Original Article

Trehalose as glucose surrogate in proliferation and cellular mobility of adult neural progenitor cells derived from mouse hippocampus

Authors: Alexandra Bertl, Victor Brantl, Norbert Scherbaum, Dan Rujescu, Jens Benninghoff

Published in: Journal of Neural Transmission | Issue 11/2019

Abstract

The disaccharide trehalose (TRE) represents a natural energy supply for distinct non-mammalian species. Evidence has shown that TRE impacts on various properties including the stabilization of protein structure and cell membranes, which are important neuroprotective features against neurodegeneration. In this study, we tested the specific effect of TRE on cell proliferation and mobilization using an established experimental paradigm of adult neural progenitor cells (NPCs) derived from murine hippocampus. NPC proliferation, both measured by growth curve analysis over 25 days and by bromodeoxyuridine (BrdU) incorporation, was not altered by adding TRE instead of GLC to the culture media. Using Boyden chamber experiments, the mobility in regular glucose-containing media did not differ from glucose-free TRE-supplemented media. Our observation suggests that TRE has the capacity to replace glucose (GLC) as energy source in neural cells in our experimental paradigm.

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Title: Trehalose as glucose surrogate in proliferation and cellular mobility of adult neural progenitor cells derived from mouse hippocampus
Authors: Alexandra Bertl
Victor Brantl
Norbert Scherbaum
Dan Rujescu
Jens Benninghoff
Publication date: 01-11-2019
Publisher: Springer Vienna
Published in: Journal of Neural Transmission / Issue 11/2019
Print ISSN: 0300-9564
Electronic ISSN: 1435-1463
DOI: https://doi.org/10.1007/s00702-019-02070-4

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Springer Medicine

Trehalose as glucose surrogate in proliferation and cellular mobility of adult neural progenitor cells derived from mouse hippocampus

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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