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01-01-2017 | Original Article

Regeneration of peritoneal mesothelial cells after placement of hyaluronate carboxymethyl-cellulose (Seprafilm^®)

Authors: Hideki Osawa, Junichi Nishimura, Masayuki Hiraki, Hidekazu Takahashi, Naotsugu Haraguchi, Taishi Hata, Masakazu Ikenaga, Kohei Murata, Hirofumi Yamamoto, Tsunekazu Mizushima, Yuichiro Doki, Masaki Mori

Published in: Surgery Today | Issue 1/2017

Abstract

Purpose

To examine the regeneration of mesothelium under a bioresorbable membrane.

Methods

A 1 cm² piece of peritoneum was resected from both sides of the abdominal wall of retired female mice. A piece of hyaluronate and carboxymethyl-cellulose (Seprafilm^®) was placed over the wound on one side and the other side was left uncovered. We evaluated the degree of adhesion and regeneration of mesothelial cells macroscopically and histologically using immunohistochemistry at different times.

Results

Macroscopically, the degree of postoperative adhesion in the treated site was significantly less than that in the untreated site. The membrane was left in place for 7 postoperative days (PODs). By POD 5, the regenerated peritoneum mesothelial cells covered part of the area and by POD 7, they had regenerated over almost all of that area in the abdominal wall.

Conclusion

The anti-adhesion membrane worked as a physical barrier to prevent postoperative adhesion until the mesothelial cells had regenerated completely. To our knowledge, this is the first study conducted to assess the regeneration of peritoneum mesothelial cells under a bioresorbable membrane using immunohistochemistry.

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Title: Regeneration of peritoneal mesothelial cells after placement of hyaluronate carboxymethyl-cellulose (Seprafilm®)
Authors: Hideki Osawa
Junichi Nishimura
Masayuki Hiraki
Hidekazu Takahashi
Naotsugu Haraguchi
Taishi Hata
Masakazu Ikenaga
Kohei Murata
Hirofumi Yamamoto
Tsunekazu Mizushima
Yuichiro Doki
Masaki Mori
Publication date: 01-01-2017
Publisher: Springer Japan
Published in: Surgery Today / Issue 1/2017
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-016-1350-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Regeneration of peritoneal mesothelial cells after placement of hyaluronate carboxymethyl-cellulose (Seprafilm^®)

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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