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Published in: Comparative Clinical Pathology 2/2021

01-04-2021 | Original Article

Cannabis sativa L. Mitigates Oxidative Stress and Cholinergic Dysfunction; and Modulates Carbohydrate Metabolic Perturbation in Oxidative Testicular Injury

Authors: Ochuko L. Erukainure, Motlalepula G. Matsabisa, Veronica F. Salau, Joseph O. Erhabor, Md. Shahidul Islam

Published in: Comparative Clinical Pathology | Issue 2/2021

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Abstract

The therapeutic potentials of Cannabis sativa L. on oxidative-induced testicular dysfunction were investigated in isolated testes of male albino rats. C. sativa leaves were subjected to sequential extraction to yield the hexane, dichloromethane (DCM), and ethanol extracts. Oxidative injury was induced by incubating testicular tissue homogenate with 0.1 mM FeSO4 for 30 min at 37 °C. Treatment was done by incubating the tissues with C. sativa extracts and 0.1 mM FeSO4 under same conditions. Induction of oxidative injury led to significant (p < 0.05) depleted levels of glutathione, superoxide dismutase, catalase, and ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) activities, while elevating malondialdehyde and nitric oxide levels, acetylcholinesterase, ATPase, glucose 6-phosphatase, fructose 1,6-biphosphatase, and amylase activities. These levels and activities were significantly (p < 0.05) reversed following treatment with the extracts. The extracts also displayed cytotoxic effect against human DU-145 prostate cancer cells. These results indicate the protective potentials of C. sativa extracts against oxidative-mediated testicular injury as shown by their ability to attenuate testicular oxidative stress, and modulate cholinergic and purinergic activities, while suppressing testicular carbohydrate dysmetabolism.
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Metadata
Title
Cannabis sativa L. Mitigates Oxidative Stress and Cholinergic Dysfunction; and Modulates Carbohydrate Metabolic Perturbation in Oxidative Testicular Injury
Authors
Ochuko L. Erukainure
Motlalepula G. Matsabisa
Veronica F. Salau
Joseph O. Erhabor
Md. Shahidul Islam
Publication date
01-04-2021
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 2/2021
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-021-03200-9

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