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01-06-2019 | Original Article

Biochemical reference intervals for the Italian Heavy Draft horse

Authors: Arianna Miglio, Chiara Morelli, Carmen Maresca, Andrea Felici, Livia Moscati, Maria Teresa Antognoni

Published in: Comparative Clinical Pathology | Issue 3/2019

Abstract

Established breed-specific reference intervals are important for monitoring the health status of horses. Serum biochemical breed-specific reference intervals have been evaluated in horses and other animal breeds, but there are no studies for the Italian Heavy Draft horse. The aim of this study was to determine biochemical reference intervals for the Italian Heavy Draft horse living in central Italy. Moreover, the comparison between different physiological status (pregnancy and no pregnancy) and age (foals and adults) has been executed. Inclusion criteria were established before blood collection. Blood samples were collected from 309 apparently healthy horses (mares, stallions and foals). Serum parameters were evaluated in the Clinical Pathology Laboratory at the Teaching Veterinary Hospital of the University of Perugia. Reference intervals were determined by using well-described, modern analytical and statistical methods (Anderson-Darling test, Mann-Withney U test). Serum biochemical RIs for the IHDH are reported. Differences between the IHDH and both sport and other working horses are evident. Significant differences are indicated between pregnant and non-pregnant mares and between foals and adults. This is the first study reporting the serum biochemical RIs in the IHDH. The results suggest that breed-specific RIs should be used for the IHDH. Moreover, significant variations of biochemical analytes have been found between subjects with different physiological status and age.

Antognoni MT, Veronesi F, Morganti G, Mangili V, Fruganti G, Miglio A (2014) Natural infection of Anaplasma platys in dogs from Umbria region (Central Italy). Vet Ital 50:49–56PubMed

Aros K, Carrasco J, Briones R, Tadich TA (2017) Haematological and serum biochemical reference values for urban-working equines in Chile. Austral J Vet Sci 49:27–33CrossRef

Bauer JE, Harvey JW, Asquith RL, McKunlty K, Kivipelto J (1984) Clinical chemistry reference values of foals during the first year of life. Equine Vet J 16:361–363CrossRefPubMed

Borges AS, Divers TJ, Stokol T, Mohammed OH (2007) Serum iron and plasma fibrinogen as indicators of systemic inflammatory diseases in horses. J Vet Intern Med 21:489–494CrossRefPubMed

Cherry SH, Filler M, Harvey H (1973) Lysozyme content of the amniotic fluid. Am J Obstet Gynecol 116:639–641CrossRefPubMed

Cray C, Belgrave RL (2014) Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses. J Equine Vet Sci 34:337–340CrossRef

Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J (2012) ASVCP reference interval guidelines: determination of the novo reference intervals in veterinary species and other related topics. Vet Clin Pathol 41:441–453CrossRefPubMed

Harvey JW, Pate MG, Kivipelto J, Asquith RL (2005) Clinical biochemistry of pregnant and nursing mares. Vet Clin Pathol 34:248–254CrossRefPubMed

Hughes KJ, Hodgson DR, Dart AJ (2004) Equine hyperlipaemia: a review. Aust Vet J 82:136–142CrossRefPubMed

Jacobsen S, Niewold TA, Halling-Thomsen M, Nanni S, Olsen E, Lindergaard C, Andersen PH (2006) Serum amyloid-A isoforms in serum and synovial fluid in horses with lipopolysaccharide-induced arthritis. Vet Immunol Immunopathol 110:325–330CrossRefPubMed

Kaneko JJ, Harvey JW, Bruss ML (1997) Blood analytes reference values in Large animals. In: Clinical Biochemistry of Domestic Animals, 5th edn. Academic Press, Cambridge, pp 890–901

Knottenbelt D (2006) Vital sings, normal values. In: Knottenbelt D (ed) Saunders equine formulary. W.B. Saunders Co, Philadelphia, USA, p 7

Mantovani R, Pigozzi G, Bittante G (2005) The Italian Heavy Draught Horse breed: origin breeding program, efficiency of the selection scheme and inbreeding. In: Conservation genetics of endangered horse breeds, 1th edn. Wageningen Academinc Publishers, Wageningen, NL Bodo, IL Alderson and B Langlois, pp 155–162

Mantovani R, Sartori C, Pigozzi G (2013) Retrospective and statistical analysis of breeding management on the Italian heavy draught horse breed. Animal 7/7:1053–1059CrossRefPubMed

McGovern K (2018a) Acute phase proteins and their clinical use in the adult horse. Equine. 2:42–47

McGovern K (2018b) Acute phase proteins and their clinical use in the adult horse. Equine. 2:42–47

Miglio A, Moscati L, Fruganti G, Pela M, Scoccia E, Valiani A, Maresca C (2013) Use of milk amyloid A in the diagnosis of subclinical mastitis in dairy ewes. J Dairy Res 80:496–502CrossRefPubMed

Miglio A, Antognoni MT, Maresca C, Moncada C, Riondato F, Scoccia E, Mangili V (2015) Serum protein concentration and protein fractions in clinically healthy Lacaune and Sarda sheep using agarose gel electrophoresis. Vet Clin Pathol 44:564–569CrossRefPubMed

Miglio A, Moscati L, Scoccia E, Maresca C, Antognoni MT, Felici A (2018) Reference values for serum amyloid A, haptoglobin, lysozyme, zinc and iron in healthy lactating Lacaune sheep. Acta Vet Scand 60:46CrossRefPubMedPubMedCentral

Miglio A, Morelli C, Maresca C, Felici A, Di Giambattista A, Antognoni MT (2019) Hematologic reference internvals for the Italian heavy draft horse. Comp Clin Pathol. https://doi.org/10.1007/s00580-019-02896-0

Monesinos P, Satuè K (2013) Iron-related paramenters in Spanish mares during pregnancy. Comp Clin Pathol 22:21–27CrossRef

Munoz A, Riber C, Trigo P, Castejòn F (2012) Age- and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Res Vet Sci 93:943–949CrossRefPubMed

Murase H, Sakai S, Kusano K, Hobo S, Nambo Y (2013) Serum zinc levels and their relationship with diseases in racehorses. J Vet Med Sci 75:37–41CrossRefPubMed

Nole-Walston (2015) How to interpret serum amyloid A concentrations. AAEP 61:130–137

Passamonti F, Veronesi F, Cappelli K, Capomaccio S, Reginato A, Miglio A, Vardi DM, Stefanetti V, Coletti M, Bazzica C, Pepe M (2015) Polysynovitis in a horse due to Borrelia burgdorferi sensu lato infection—case study. Ann Agr Env Med 22:247–250CrossRef

Pritchard JC, Burn CC, Barr ASR, Whay HR (2009) Haematological and serum biochemical reference values for apparently healthy working horses in Pakistan. Resw Vet Sci 87:389–395CrossRef

Roberts MC (1974) Total serum cholesterol levels in the horse. Br Vet J 130:xvi–xviiiCrossRefPubMed

Sample SH, Fox KM, Wunn D, Roth E, Friedrichs KR (2015) Hematological and biochemical reference intervals for adult Fresian horses from North America. Vet Clin Pathol 44:194–199CrossRefPubMed

Sotirov L (2004) Lysozyme and complement concentrations in horses, donkeys and mules. Revue Med Vet 155:221–225

Spada E, Antognoni MT, Proverbio D, Ferro E, Mangili V, Miglio A (2015) Haematological and biochemical reference intervals in adult Maine coon cat blood donors. J Feline Med Surg 17:1020–1027CrossRefPubMed

Stockham SL (1995) Interpretation of equine serum biochemical profile results. Vet Clin North Am Equine Pract 11:391–414CrossRefPubMed

Taira T, Fujinaga T, Okumura M, Yamashita K, Tsunoda N, Mizuno S (1992) Equine haptoglobin: isolation, characterization, and the effects of ageing, delivery and inflammation on its serum concentration. J Vet Med Sci 54:435–442CrossRefPubMed

Vincze B, Kutasi O, Baska F, Szenci O (2015) Pregnancy-associated changes of serum biochemical values in Lipizzaner broodmares. Acta Vet Hung 63:303–316CrossRefPubMed

Wang H, Hu YF, Hao JH, Chen YH, Su PY, Wang Y, Yu Z, Fu L, Xu YY, Zhang C, Tao FB, Xu DX (2015) Maternal zinc deficiency during pregnancy elevates the risks of fetal growth restriction: a population-based birth cohort study. Sci Rep 5:11262CrossRefPubMedPubMedCentral

Wittwer F (2012) Manual de patologìa clinica veterinaria (manual of veterinary clinical pathology), 2nd edn. Imprenta Amèrica, Valdiva, Chile

Title: Biochemical reference intervals for the Italian Heavy Draft horse
Authors: Arianna Miglio
Chiara Morelli
Carmen Maresca
Andrea Felici
Livia Moscati
Maria Teresa Antognoni
Publication date: 01-06-2019
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 3/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02930-1

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