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01-08-2019 | Albendazole | Original Article

Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide

Authors: Abdollah Rafiei, Shahrzad Soltani, Zahra Ramezani, Mohammad Reza Abbaspour, Ali Jelowdar, Mehdi Sagha Kahvaz

Published in: Comparative Clinical Pathology | Issue 4/2019

Abstract

Cystic echinococcosis (CE), a serious public health problem worldwide, is caused by the larval stage of Echinococcus granulosus (E. granulosus). Despite recent progress in the CE medical treatment, this subject still remains as a priority of research. Albendazole (ABZ) is a drug of choice for the treatment of hydatidosis. Its use as an antiparasitic agent with the most efficacy is limited by the lack of water solubility. Therefore, the aim of the present study was to compare in vitro efficacy of free and solid lipid nanoparticles (SLNs) of ABZ and albendazole sulfoxide (ABZSO) against fertile and infertile hydatid cysts in terms of ultrastructural changes. ABZ-SLN and ABZSO-SLN were produced by micro emulsification and high shear homogenization technique and the physicochemical characterization of the product was evaluated. Then, the structural alterations occurring due to treatment with free and SLN formulations against fertile and infertile sheep liver hydatid cysts were assessed by optical and transmission electron microscopy (TEM) examinations. Untreated control groups revealed the typical appearance of hydatid cysts; in contrast, all treated cysts exhibited degenerative alterations with various rates. Loading ABZ and ABZSO into SLN leads to greater structural changes. In both prepared formulations, better efficacy was observed in fertile and small cysts.

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Title: Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide
Authors: Abdollah Rafiei
Shahrzad Soltani
Zahra Ramezani
Mohammad Reza Abbaspour
Ali Jelowdar
Mehdi Sagha Kahvaz
Publication date: 01-08-2019
Publisher: Springer London
Keyword: Albendazole
Published in: Comparative Clinical Pathology / Issue 4/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02925-y

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Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide

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