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01-11-2018 | Original Article

Comparative study on the interaction of eugenol, Butylated hydroxylanisole, and Butylated hydroxyl toluene with some crucial enzymes linked to erectile dysfunction

Authors: Stephen Adeniyi Adefegha, Bathlomew Maduka Okeke, Ganiyu Oboh

Published in: Comparative Clinical Pathology | Issue 6/2018

Abstract

This study investigated and compared the effect of natural phenolic compound (eugenol) and synthetic phenolic compounds [butylated hydroxylanisole (BHA) and butylated hydroxyl toluene (BHT)] on some crucial enzymes (angiotensin-1-converting enzyme (ACE) and arginase) linked to erectile dysfunction (ED) in vitro. Furthermore, the effects of the compounds were assessed on several antioxidant parameters including DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) free radical scavenging ability, ferric reducing power, and Fe²⁺ chelating ability. Ten millimolar each of the phenolic compounds was prepared in a mixture of ethanol and water (1:1 v/v), and their interactions with ACE and arginase was assessed. Furthermore, ferric-reducing antioxidant property (FRAP), Fe²⁺-chelating ability, DPPH, and ABTS radicals scavenging abilities were determined. The results revealed that eugenol, BHT, and BHA inhibited ACE and arginase activities in dose-dependent manner. However, eugenol showed the highest inhibitory effect on both enzymes. In addition, eugenol significantly (p < 0.05) had higher FRAP, ABTS scavenging, and Fe²⁺ chelating abilities when compared with the synthetic compounds (BHT and BHA). The strong inhibitory effects and antioxidative properties of eugenol suggest eugenol as a promising natural phenolic compound could be employed as an alternative food additive and erectogenic agent in ED management/treatment.

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Title: Comparative study on the interaction of eugenol, Butylated hydroxylanisole, and Butylated hydroxyl toluene with some crucial enzymes linked to erectile dysfunction
Authors: Stephen Adeniyi Adefegha
Bathlomew Maduka Okeke
Ganiyu Oboh
Publication date: 01-11-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 6/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2796-0

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Comparative study on the interaction of eugenol, Butylated hydroxylanisole, and Butylated hydroxyl toluene with some crucial enzymes linked to erectile dysfunction

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