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Open Access 01-09-2018 | Original Article

Respiratory and GIT tract immune responses of broiler chickens following experimental infection with Newcastle disease’s virus

Authors: Hadi Rohollahzadeh, Hassan Nili, Keramat Asasi, Saeed Mokhayeri, Amir Hossein Asl Najjari

Published in: Comparative Clinical Pathology | Issue 5/2018

Abstract

Newcastle disease causes a lymphoproliferative response in the tracheal and intestinal mucosa of the infected birds. In this study, the Hitchner B1 and I-2 vaccine and challenging of ND field strains were used to evaluate the populations of T lymphocyte subsets infiltrated intestinal and tracheal, also to shed some light on cell-mediated immune response using enzyme-linked immunosorbent assay (ELISA) detecting chicken’s serum interferon-γ. Three hundred-day-old broilers were randomly divided into four groups. Groups 1 and 2 received I-2 and B1 vaccines, respectively, while groups 3 and 4 were challenged-unvaccinated and unchallenged-unvaccinated groups. Blood samples were taken from five random chicks and were then tested with ELISA test. Three chicks of each group were euthanized after vaccine administration and also challenging with acute virus. Interferon-γ changes were significant in time (p < 0.001). Totally, there was no significant difference between I-2 and B1 groups. The number of CD3+, CD4+, and CD8+ cells of I-2 and B1 vaccinated group’s intestine and the trachea samples was significantly increased compared with the negative control group (p < 0.001). The results indicated the significant increase in CD4+ and CD8+ in intestinal and tracheal tissues, while the level of interferon-γ of the vaccinated group was more than the unvaccinated one. Finding no significant differences between the vaccinated groups indicated the potential of both vaccines in producing CD4+ and CD8+ in the tracheal and intestinal tissues and the equality of interferon-γ production in the sera.

Agrawal PK, Reynolds DL (1991) Evaluation of the cell-mediated immune response of chickens vaccinated with Newcastle disease virus as determined by the under-agarose leukocyte-migration-inhibition technique. Avian Dis 35:360–364CrossRefPubMed

Allan WH, Lancaster JE, Toth B (1978) Newcastle disease vaccines—their production and use FAO animal production series no. 10. FAO, Rome

Awad F, Forrester A, Baylis M, Lemiere S, Jones R, Ganapathy K (2014) Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks. Res Vet Sci 98:127–133CrossRefPubMed

Alexander DJ, Manvell RJ, Parsons G (2006) Newcastle disease virus (strain Herts 33/56) in tissues and organs of chickens infected experimentally. Avian Pathol 35(2):99/101CrossRef

Bar-Shira E, Sklan D, Friedman A (2003) Establishment of immune competence in the avian GALT during the immediate post-hatch period. Dev Comp Immunol 27:147–157CrossRefPubMed

Bensink Z, Spradbrow P (1999) Newcastle disease virus strain I-2 a prospective thermostable vaccine for use in developing countries. Vet Microbiol 68:131–139CrossRefPubMed

Bing-guo P, Xiao L, Li-li S, Shi-fu K, Lei L, Hai-yan H, Guo-hua Y, Yu-hang W, Zhuo-yue W, Jiu-hua S, Ning-yi J (2011) Activity of T cells stimulated by hemagglutinin-neuraminidase of Newcastle disease viruse in vivo. Chem Res Chin Univ 27:455–460

Bell JG (2001) A comparison of the different vaccine available for the control of Newcastle disease in village chickens. ACIAR Proc 103:56–60

Breed DGJ, Dorrestein J, Schetters TPM, Waart LVD, Rijke E, Vermeulen AN (1997) Peripheral blood lymphocytes from Eimeria tenella infected chickens produce gamma-γ after stimulation in vitro. Parasite Immunol 19:127–135CrossRefPubMed

Chan MM, Chen CH, Ager LL, Cooper MD (1988) Identification of the avian homologues of mammalian CD4 and CD8 antigens. J Immunol 140:2133–2138PubMed

Cornax I, Miller PJ, Afonso CL (2012) Characterization of live LaSota vaccine straine induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype. Avian Dis 56:464–470CrossRefPubMed

Farrel HE, Davis-Poynter NJ (1998) From sabotage to camouflage: viral evasion of cytotoxic T lymphocyte and natural killer cell-mediated immunity. Cell Dev Biol 9:369–378CrossRef

Garcia-Camacho L, Schat KA, Brooks JR, Bounous DI (2003) Early cell-mediated immune responses to Marek’s disease virus in two chicken lines with defined major histocompatibility complex antigens. Vet Immunol Immunopathol 95:145–153CrossRefPubMed

Ghumman JS, Bankowski RA (1976) In vitro DNA synthesis in lymphocytes from turkeys vaccinated with La Sota, TC, and inactivated Newcastle disease vaccines. Avian Dis 20:18–31CrossRefPubMed

Glick B (2000) Immunophysiology. In: Whittow GC (ed) Sturkie′s Avian Physiology, 5th edn. Academic Press, London, pp 657–667CrossRef

Kaiser P, Staheli P (2008) Avian cytokines and chemokines in avian immunology. Elsevier, London, pp 203–222CrossRef

Kamran H, Mohamed Faizal AC, Sangitha S, Niroshan TD, Leah RR, Shayan S (2010) Vaccine-induced host responses against very virulent Marek’s disease virus infection in the lungs of chickens. Vaccine 28:5565–5572CrossRef

Karaca K, Kim IJ, Reddy SK, Sharma JM (1996) Nitric oxide inducing factor as a measure of antigen and mitogen-specific T cell responses in chickens. J Immunol Methods 192:97–103CrossRefPubMed

Khalifeh MS, Amawi MM, Abu-Basha EA, Yonis IB (2009) Assessment of humoral and cellular-mediated immune response in chickens treated with tilmicosin, florfenicol, or enrofloxacin at the time of Newcastle disease vaccination. Poult Sci 88:2118–2124CrossRefPubMed

Lambrecht B, Gonze M, Morales D, Meulemans G, Van Den Berg TP (2000) Comparison of biological activities of natural and recombinant chicken interferongamma. Vet Immunol Immunopathol 70:257–267CrossRef

Lambrecht B, Gonze M, Meulemans G, van den Berg T (2004) Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-gamma in response to mitogen and recall Newcastle disease viral antigen stimulation. Avian Pathol 33:343–350CrossRefPubMed

Lillehoj HS, Trout JM (1993) Coccidia: a review of recent advances on immunity and vaccine development. Avian Pathol 22:3–31CrossRefPubMed

Marino OC, Hanson RP (1987) Cellular and humoral response of in ovobursectomized chickens to experimental challenge with velogenic Newcastle disease virus. Avian Dis 31:293–301CrossRefPubMed

Mateu de A, Robert JH, Richard H, Joan K, David S, Stephen M, Federco AZ (1998). Quantitative detecteion of porcine interferon-gamma in response to mitogen, superantigen and recal viral antigen. Vet Immunol Immunopathol 61:265–277

Martin A, Lillehoj HS, Kaspers B, Bacon LD (1994) Mitogen-induced lymphocyte proliferation and interferon production following coccidia infection. Avian Dis 38:262–268CrossRefPubMed

Nili H, Asasi K, Habibi GH (2005) Thermostability and efficacy of I-2 vaccine against Newcastle disease (ND) in Iran: a pilot study. Iran J Vet Res 6:42–46

Noraup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR (2011) Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol 74:23–30CrossRef

OIE (2012) Manual of diagnostic tests and vaccines for terrestrial animals: mammals, birds and bees. Biological Standards Commission, Vol. 1, Part 2, Chapter 2.03.14. OIE, Paris, pp 1–19

Omar AR, Schat KA (1997) Characterization of Marek’s disease herpes virus-specific cytotoxic T lymphocytes in chickens inoculated with a non-oncogenic vaccine strain of MDV. Immunology 90:579–585CrossRefPubMedPubMedCentral

Parvizi P, Read L, Abdul-Careem MF, Lusty C, Sharif S (2009) Cytokine gene expression in splenic CD4(+) and CD8(+) T-cell subsets of chickens infected with Marek’s disease virus. Viral Immunol 22(1):31–38CrossRefPubMed

Peiris JS, Cheung CY, Leung CY, Nicholls JM (2009) Innate immune responses to influenza A H5N1: friend or foe? Trends Immunol 30:574e84CrossRef

Peiris JS, Hui KP, Yen HL (2010) Host response to influenza virus: protection versus immunopathology. Curr Opin Immunol 22:475–481CrossRefPubMedPubMedCentral

Prowse SJ, Pallister J (1989) Interferon release as a measure of the T-cell response to coccidial antigens in chickens. Avian Pathol 18:619–630CrossRefPubMed

Rauw F, Gardin Y, Palya V, van Borm S, Gonze M, Lemaire S (2009) Humoral, cell mediated and mucosal immunity induced by oculo-nasal vaccination of one day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines. Vaccine 27:3631–3642CrossRefPubMed

Reynolds DL, Maraqa AD (2000) Protective immunity against Newcastle disease: the role of cell-mediated immunity. Avian Dis 44:145–154CrossRefPubMed

Rothel JS, Jones SL, Corner LA, Cox JC, Wood PR (1990) A sandwich enzyme immunoassay for bovine interferon-g and its use for the detection of tuberculosis in cattle. Aust Vet J 67:134–137CrossRefPubMed

Rue CA, Susta L, Cornax I, Brown CC, Kapczynski DR, Suarez DL, King DJ, Miller PJ, Afonso CL (2011) Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J Gen Virol 92:931–939CrossRefPubMed

Russell PH, Dwivedi PN, Davison TF (1997). The effects of cyclosporin A and cyclophosphamide on the opulations of B and T cells and virus in the Harderian gland of chickens vaccinated with the Hitchner B1 strain of Newcastle disease virus. Vet Immunol Immunopathol 60(1-2):171–85.

Saalmuller A, Redhasse MJ, Buhring HJ, Jonjic S, Koszinowski UH (1997) Simultaneous expression on CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes. Eur J Immunol 17:1297–1301CrossRef

Seal BS, King DJ, Sellers HS (2000) The avian response to Newcastle disease virus. Dev Comp Immunol 24:257–268CrossRefPubMed

Sharma JM (1999) Introduction to poultry vaccines and immunity. Adv Vet Med 41:481–494CrossRefPubMed

Silke R, Christine H (2005) Differences in immunopathogenesis of infectious bursal disease virus (IBDV) following in ovo and post-hatch vaccination of chickens. Vet Immunol Immunopathol 106:139–150.

Songserm T, Engel B, van Roozelar DJ, Kok GL, Pijpers A, Pol JMA, ter Huurne AAHM (2000) Cellular immune response in the small intestine of two broiler chicken lines orally inoculated with malabsorption syndrome homogenates. Vet Immunol Immunopathol 85:51–62CrossRef

Staeheli P, Puehler F, Schneider K, Göbel TW, Kaspers B (2001) Cytokines of birds: conserved functionsea largely different look. J Interf Cytokine Res 21:993–1010CrossRef

Susta L, Cornax I, Diel DG, Garcia SC, Miller PJ, Liu X et al (2013) Expression of interferon gamma by a highly virulent strain of Newcastle disease virus decreases its pathogenicity in chickens. Microb Pathog 61–62:73–83CrossRefPubMed

Swayne DE, Glisson JR, Mc Dougald LR, Nolan LK, Suarez DL, Nair VL (2013) “Adenoviruses (Egg Drop Syndrome and Related Infectious) Diseases of Poultry”, 13th edn. p 1394–1430

Takao K, Saeko W, Yasuhiro T, Mitsuru K, Jyoji Y, Sadashige S (1999) Kinetics of lymphocytic subsets in chicken tracheal lesions infected with infectious bronchitis virus. Osaka Prefecture University, Sakai, pp 599–8531

Usman M (2002) Effects of vaccination of chickens against Newcastle disease with thermostable V4 and Lasota vaccines using different grains and their brans as vehicle

Vainio O, Lassila O (1989) Chicken T cell differentiation antigens and cell interactions. Crit Rec Poult Biol 2:97–102

White PG, Appleton GS (1953) The speed of immunity response following vaccination with the B1 strain of Newcastle disease virus. Am J Vet Res 14:609–611PubMed

Whiteside TL (1994) Cytokine measurements and interpretation of cytokine assays in human disease. J Clin Immunol 14:327–339CrossRefPubMed

Whitmire JK, Tan JT, Whitton JL (2005) Interferon-gamma acts directly on CD8+ T cells to increase their abundance during virus infection. J Exp Med 201:1050–1053CrossRef

Xiaofei Z, Xiaowen Z, Qian Y (2007) Effect of compound mucosal immune adjuvant on mucosal and systemic immune responses in chicken orally vaccinated with attenuated Newcastle-disease vaccine. Vaccine 25:3254–3262CrossRef

Yin J, Jin H, Kang Y, Xiao C, Zhao L, Li X, Ding Z, Yang F, Zhu Q, Wang B (2006) Efficacy of modified levamisole adjuvant on inactivated virus vaccine. Viral Immunol 19:525–535CrossRefPubMed

Title: Respiratory and GIT tract immune responses of broiler chickens following experimental infection with Newcastle disease’s virus
Authors: Hadi Rohollahzadeh
Hassan Nili
Keramat Asasi
Saeed Mokhayeri
Amir Hossein Asl Najjari
Publication date: 01-09-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 5/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2728-z

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Respiratory and GIT tract immune responses of broiler chickens following experimental infection with Newcastle disease’s virus

Abstract

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Abstract

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