Top

Published in:

Open Access 01-07-2018 | Original Article

BIO (6-bromoindirubin-3′-oxime) GSK3 inhibitor induces dopaminergic differentiation of human immortalized RenVm cells

Authors: Mitra Soleimani, Nazem Ghasemi, Fatemeh Mohammadi Chamnari

Published in: Comparative Clinical Pathology | Issue 4/2018

Abstract

Parkinson’s disease (PD) is one of the most neurodegenerative disorders which can lead to severe neural disability and neurological defects. Cell-based therapy using fully differentiated cells is a new method for the treatment of this abnormal condition. In the present study, we investigated the effects of 6-bromoindirubin-3′-oxime (BIO) on dopaminergic differentiation of human immortalized RenVm cells in order to obtain a set of fully differentiated cells for transplantation in Parkinson’s disease. To this end, the immortalized RenVm cells were induced to dopaminergic differentiation using a neuro basal medium supplemented with N2 and different concentrations (75, 150, 300, 600, and 1200 nM) of BIO for 4, 8, and 12 days. The efficiency of dopaminergic differentiation was determined using immunocytochemistry for tyrosine hydroxylase expressions. In addition, the expression of a β-catenin marker was measured using the western blot technique. The results of immunocytochemistry revealed that the mean percentage of Tuj1- and TH-positive sells in 150- and 300-nM-BIO-treated groups was significantly increased compared to that of other groups (p ≤ 0.01). In addition, the expression of the β-catenin marker was higher in these groups as compared with that of other groups. Overall, BIO through its effect on the Wnt-Frizzled signaling pathway can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner.

Amariglio N, Hirshberg A, Scheithauer BW, Cohen Y, Loewenthal R, Trakhtenbrot L, Paz N, Koren-Michowitz M, Waldman D, Leider-Trejo L, Toren A (2009) Donor-derived brain tumor following neural stem cell transplantation in an ataxia telangiectasia patient. PLoS Med 6:e1000029CrossRefPubMedPubMedCentral

Bantubungi K, Blum D, Cuvelier L, Wislet-Gendebien S, Rogister B, Brouillet E, Schiffmann SN (2008) Stem cell factor and mesenchymal and neural stem cell transplantation in a rat model of Huntington’s disease. Mol Cell Neurosci 37:454–470CrossRefPubMed

Blurton-Jones M, Kitazawa M, Martinez-Coria H, Castello NA, Müller FJ, Loring JF, Yamasaki TR, Poon WW, Green KN, LaFerla FM (2009) Neural stem cells improve cognition via BDNF in a transgenic model of Alzheimer disease, vol 106. Proceedings of the National Academy of Sciences, pp 13594–13599

Chizhikov VV, Millen KJ (2005) Roof plate-dependent patterning of the vertebrate dorsal central nervous system. Dev Biol 277:287–295CrossRefPubMed

Chong ZZ, Maiese K (2004) Targeting WNT, protein kinase B, and mitochondrial membrane integrity to foster cellular survival in the nervous system. Histol Histopathol 19:495PubMedPubMedCentral

Dono R, Faulhaber J, Galli A, Zuniga A, Volk T, Texido G, Zeller R, Ehmke H (2002) FGF2 signaling is required for the development of neuronal circuits regulating blood pressure. Circ Res 90:e5–e10CrossRefPubMed

Du SJ, Purcell SM, Christian JL, McGrew LL, Moon RT (1995) Identification of distinct classes and functional domains of Wnts through expression of wild-type and chimeric proteins in Xenopus embryos. Mol Cell Biol 15:2625–2634CrossRefPubMedPubMedCentral

Fukumoto S, Hsieh CM, Maemura K, Layne MD, Yet SF, Lee KH, Matsui T, Rosenzweig A, Taylor WG, Rubin JS, Perrella MA (2001) Akt participation in the Wnt signaling pathway through Dishevelled. J Biol Chem 276:17479–17483CrossRefPubMed

Ghasemi N, Razavi S, Mardani M, Esfandiari E, Salehi H, Esfahani SH (2014) Transplantation of human adipose-derived stem cells enhances remyelination in lysolecithin-induced focal demyelination of rat spinal cord. Mol Biotechnol 56:470–478CrossRefPubMed

Holnthoner W, Pillinger M, Gröger M, Wolff K, Ashton AW, Albanese C, Neumeister P, Pestell RG, Petzelbauer P (2002) Fibroblast growth factor-2 induces Lef/Tcf-dependent transcription in human endothelial cells. J Biol Chem 277:45847–45853CrossRefPubMed

Hsieh JC (2004) Specificity of WNT-receptor interactions. Front Biosci 9:333–1338CrossRef

Jones SE, Jomary C, Grist J, Stewart HJ, Neal MJ (2000) Modulated expression of secreted Frizzled related proteins in human retinal degeneration. Neuroreport 11:3963–3967PubMed

Kiecker C, Niehrs C (2001) A morphogen gradient of Wnt/β-catenin signalling regulates anteroposterior neural patterning in Xenopus. Development 128:4189–4201PubMed

Kishida M, Hino SI, Michiue T, Yamamoto H, Kishida S, Fukui A, Asashima M, Kikuchi A (2001) Synergistic activation of the Wnt signaling pathway by Dvl and casein kinase Iε. J Biol Chem 276:33147–33155CrossRefPubMed

Liu K, Wu X, Luo F (2015) The mechanisms and effects of GSK-3 {beta} inhibitor on cellular multidrug resistance in colon cancer cells. ASCO Annu Meet Proc 33:e13592

Mao J, Wang J, Liu B, Pan W, Farr GH, Flynn C, Yuan H, Takada S, Kimelman D, Li L, Wu D (2001) Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway. Mol Cell 7:801–809CrossRefPubMed

Michiue T, Fukui A, Yukita A, Sakurai K, Danno H, Kikuchi A, Asashima M (2004) XIdax, an inhibitor of the canonical Wnt pathway, is required for anterior neural structure formation in Xenopus. Dev Dyn 230:79–90CrossRefPubMed

Nelson WJ, Nusse R (2004) Convergence of Wnt, ß-catenin, and cadherin pathways. Science 303:1483–1487CrossRefPubMedPubMedCentral

Panhuysen M, Weisenhorn DM, Blanquet V, Brodski C, Heinzmann U, Beisker W, Wurst W (2004) Effects of Wnt1 signaling on proliferation in the developing mid-/hindbrain region. Mol Cell Neurosci 26:101–111CrossRefPubMed

Park HJ, Lee PH, Bang OY, Lee G, Ahn YH (2008) Mesenchymal stem cells therapy exerts neuroprotection in a progressive animal model of Parkinson’s disease. J Neurochem 107:141–151CrossRefPubMed

Patapoutian A, Reichardt LF (2000) Roles of Wnt proteins in neural development and maintenance. Curr Opin Neurobiol 10:392–399CrossRefPubMedPubMedCentral

Pinson KI, Brennan J, Monkley S, Avery BJ, Skarnes WC (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature 407:535–538CrossRefPubMed

Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10:55–63CrossRefPubMed

Seedat S, Kesler S, Niehaus DJ, Stein DJ (2000) Pathological gambling behaviour: emergence secondary to treatment of Parkinson’s disease with dopaminergic agents. Depress Anxiety 11:185–186CrossRefPubMed

Wahner AD, Bronstein JM, Bordelon YM, Ritz B (2007) Nonsteroidal anti-inflammatory drugs may protect against Parkinson disease. Neurology 69(19):1836–1842CrossRefPubMed

Wehrli M, Dougan ST, Caldwell K, O'keefe L, Schwartz S, Vaizel-Ohayon D, Schejter E, Tomlinson A, DiNardo S (2000) Arrow encodes an LDL-receptor-related protein essential for Wingless signalling. Nature 407:527–530CrossRefPubMed

Weiss ML, Medicetty S, Bledsoe AR, Rachakatla RS, Choi M, Merchav S, Luo Y, Rao MS, Velagaleti G, Troyer D (2006) Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson’s disease. Stem Cells 24:781–792CrossRefPubMed

Title: BIO (6-bromoindirubin-3′-oxime) GSK3 inhibitor induces dopaminergic differentiation of human immortalized RenVm cells
Authors: Mitra Soleimani
Nazem Ghasemi
Fatemeh Mohammadi Chamnari
Publication date: 01-07-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 4/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2696-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

BIO (6-bromoindirubin-3′-oxime) GSK3 inhibitor induces dopaminergic differentiation of human immortalized RenVm cells

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 4/2018

Bluetongue virus seropositivity and some risk factors affecting bluetongue virus infection in sheep flocks

A novel pathology of a mediastinal neoplasm

Glyphosate-based herbicide induces toxic effects on human adipose-derived mesenchymal stem cells grown in human plasma

Assessment of two abdominal mass closure methods using fishing line in rabbit model: clinical and pathological study

Effects of electrofishing on stress response of wild carp (Cyprinus carpio, L.)

Human MTHFR-C677T gene transition increased risk of Alzheimer disease: a case-control study which performed by optimized PCR-CTPP method