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Published in: Comparative Clinical Pathology 1/2018

01-01-2018 | Original Article

Serum 8-hydroxydeoxyguanosine and aldose reductase C-106T polymorphism in type 2 diabetes mellitus and its relation to complications in Egyptian patients

Authors: Mostafa Mohamed Mansour, Mona Abo Bakr El-Hussiny, Nawal Abd El-Galeel Ghareeb, Hamdy Fouad Ali Marzouk, Nabil Ismael Lumon

Published in: Comparative Clinical Pathology | Issue 1/2018

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Abstract

Diabetes mellitus (DM) is an inherited disease, which can affect every organ in the body. 8-hydroxydeoxyguanosine (8-OHdG) is a biomarker for oxidative DNA damage. Occurrence of C-106T polymorphism of the aldose reductase (ALR) gene in type II diabetic patients suffering from diabetic microvascular complications has been reported. The aim of the present work was to assess the association between oxidative stress and C(-106)T ALR gene polymorphism and the incident of type 2 diabetes (T2D) and its complications. The current study was conducted on 200 subjects classified into 150 T2D patients and 50 healthy control subjects. Quantitative determination of human 8-OHdG was done using enzyme-linked immunosorbent assay (ELISA) technique. ALR C(-106)T polymorphism was assessed by PCR/RFLP method. Levels of 8-OHdG were significantly increased in diabetic patients when compared to control subjects and in diabetic patients with complications when compared to those without complications (P < 0.001 or all). CT genotype was associated with susceptibility to development of T2D in Egyptian population. Nevertheless, this association has not been observed in diabetic complications. 8-OHdG level is significantly increased in diabetic group when compared to the control group and in diabetic patients with complications when compared to diabetic patients without complications. C(-106)T gene polymorphisms were associated with susceptibility to development diabetes, but it has no associations in development of complications.
Literature
go back to reference Al-Aubaidy HA, Jelinek HF (2011) Oxidative DNA damage and obesity in type 2 diabetes mellitus. Eur J Endocrinol 164(6):899–904CrossRefPubMed Al-Aubaidy HA, Jelinek HF (2011) Oxidative DNA damage and obesity in type 2 diabetes mellitus. Eur J Endocrinol 164(6):899–904CrossRefPubMed
go back to reference American Diabetes Association (2012) Diagnosis and classification of diabetes mellitus. Diabetes Care 35(Suppl.1):S64–S71CrossRef American Diabetes Association (2012) Diagnosis and classification of diabetes mellitus. Diabetes Care 35(Suppl.1):S64–S71CrossRef
go back to reference Bonakdaran S, Hami M, Shakeri M (2011) Hyperuricemia and albuminuria in patients with type 2 diabetes mellitus. Iran J Kidney Dis 5:21–26PubMed Bonakdaran S, Hami M, Shakeri M (2011) Hyperuricemia and albuminuria in patients with type 2 diabetes mellitus. Iran J Kidney Dis 5:21–26PubMed
go back to reference Busik JV, Mohr S, Grant MB (2008) Hyperglycemia-induced reactive oxygen species toxicity to endothelial cells is dependent on paracrine mediators. Diabetes 57(7):1952–1965CrossRefPubMedPubMedCentral Busik JV, Mohr S, Grant MB (2008) Hyperglycemia-induced reactive oxygen species toxicity to endothelial cells is dependent on paracrine mediators. Diabetes 57(7):1952–1965CrossRefPubMedPubMedCentral
go back to reference Cahyono JA, Sadewa AH, Tasmini (2011) Aldose reductase genetic polymorphism is a risk factor of diabetics retinopathy among type 2 diabetes mellitus in Yogyakarta, Indonesia. J Med Sci 43(2):57–63 Cahyono JA, Sadewa AH, Tasmini (2011) Aldose reductase genetic polymorphism is a risk factor of diabetics retinopathy among type 2 diabetes mellitus in Yogyakarta, Indonesia. J Med Sci 43(2):57–63
go back to reference Cambiaso CL, Collet-Cassart D, Lievens M (1988) Immunassay of low concentrations of albumin in urine by latex particle counting. Clin Chem 34(2):416–418PubMed Cambiaso CL, Collet-Cassart D, Lievens M (1988) Immunassay of low concentrations of albumin in urine by latex particle counting. Clin Chem 34(2):416–418PubMed
go back to reference Cui W, Du B, Cui Y, Kong L, Wu H, Wang Y, Miao L, Zhou W (2015) Is rs759853 polymorphism in promoter of aldose reductase gene a risk factor for diabetic nephropathy? A meta-analysis. Eur J Med Res 20:14–23CrossRefPubMedPubMedCentral Cui W, Du B, Cui Y, Kong L, Wu H, Wang Y, Miao L, Zhou W (2015) Is rs759853 polymorphism in promoter of aldose reductase gene a risk factor for diabetic nephropathy? A meta-analysis. Eur J Med Res 20:14–23CrossRefPubMedPubMedCentral
go back to reference Deckert T, Feldt-Rasmussen B, Borch-Johnsen K, Jensen T, Kofoed-Enevoldsen A (1989) Albuminuria reflects widespread vascular damage. The Steno hypothesis. Diabetologia 32(4):219–226CrossRefPubMed Deckert T, Feldt-Rasmussen B, Borch-Johnsen K, Jensen T, Kofoed-Enevoldsen A (1989) Albuminuria reflects widespread vascular damage. The Steno hypothesis. Diabetologia 32(4):219–226CrossRefPubMed
go back to reference Demaine A, Cross D, Millward A (2000) Polymorphisms of the aldose reductase gene and susceptibility to retinopathy in type 1 diabetes mellitus. Invest Ophthalmol Vis Sci 41:4064–4068PubMed Demaine A, Cross D, Millward A (2000) Polymorphisms of the aldose reductase gene and susceptibility to retinopathy in type 1 diabetes mellitus. Invest Ophthalmol Vis Sci 41:4064–4068PubMed
go back to reference Drummond K, Mauer M (2002) The early natural history of nephropathy in type 1 diabetes, II: early renal structural changes in type 1 diabetes. Diabetes 51:1580–1587CrossRefPubMed Drummond K, Mauer M (2002) The early natural history of nephropathy in type 1 diabetes, II: early renal structural changes in type 1 diabetes. Diabetes 51:1580–1587CrossRefPubMed
go back to reference El-Wassef M, El-Saeed GSM, El-Tokhy SE, Raslan HM, Tawfeek S, Siam I, Salem SI (2012) Oxidative DNA damage in patients with type 2 diabetes mellitus. Diabetol Croat 41(4):121–127 El-Wassef M, El-Saeed GSM, El-Tokhy SE, Raslan HM, Tawfeek S, Siam I, Salem SI (2012) Oxidative DNA damage in patients with type 2 diabetes mellitus. Diabetol Croat 41(4):121–127
go back to reference Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 23(5):599–622CrossRefPubMed Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 23(5):599–622CrossRefPubMed
go back to reference Fan Z, Cai Q, Chen Y et al (2016) Association of the transcription factor 7 like 2 (TCF7L2) polymorphism with diabetic nephropathy risk: a meta-analysis. Medicine 95(11):e3087CrossRefPubMedPubMedCentral Fan Z, Cai Q, Chen Y et al (2016) Association of the transcription factor 7 like 2 (TCF7L2) polymorphism with diabetic nephropathy risk: a meta-analysis. Medicine 95(11):e3087CrossRefPubMedPubMedCentral
go back to reference Fiorentino TV, Prioletta A, Zuo P et al (2013) Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des 19(32):5695–5703CrossRefPubMed Fiorentino TV, Prioletta A, Zuo P et al (2013) Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des 19(32):5695–5703CrossRefPubMed
go back to reference Gerstein H, Mann J, Yi Q, Zinman B, Dinneen SF, Hoogwerf B, Hallé JP, Young J, Rashkow A, Joyce C, Nawaz S, Yusuf S (2001) Albuminuria and risk of cardiovascular events, death, and heart failure in diabetic and nondiabetic individuals. JAMA 286(4):421–426CrossRefPubMed Gerstein H, Mann J, Yi Q, Zinman B, Dinneen SF, Hoogwerf B, Hallé JP, Young J, Rashkow A, Joyce C, Nawaz S, Yusuf S (2001) Albuminuria and risk of cardiovascular events, death, and heart failure in diabetic and nondiabetic individuals. JAMA 286(4):421–426CrossRefPubMed
go back to reference Grant SF, Thorleifsson G, Reynisdottir I et al (2006) Variant of transcription factor 7-like 2 (TCF7L2) gene confers risk of type 2 diabetes. Nat Genet 38:320–323CrossRefPubMed Grant SF, Thorleifsson G, Reynisdottir I et al (2006) Variant of transcription factor 7-like 2 (TCF7L2) gene confers risk of type 2 diabetes. Nat Genet 38:320–323CrossRefPubMed
go back to reference Gupta DK, Verma LK, Khosla PK, Dash SC (1991) The prevalence of microalbuminuria in diabetes: a study from North India. Diabetes Res Clin Pract 12:125–128CrossRefPubMed Gupta DK, Verma LK, Khosla PK, Dash SC (1991) The prevalence of microalbuminuria in diabetes: a study from North India. Diabetes Res Clin Pract 12:125–128CrossRefPubMed
go back to reference Inoguchi T, Li P, Umeda F, Yu HY, Kakimoto M, Imamura M, Aoki T, Etoh T, Hashimoto T, Naruse M (2000) High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C—dependent activation of NAD (P) H oxidase in cultured vascular cells. Diabetes 49(11):1939–1945CrossRefPubMed Inoguchi T, Li P, Umeda F, Yu HY, Kakimoto M, Imamura M, Aoki T, Etoh T, Hashimoto T, Naruse M (2000) High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C—dependent activation of NAD (P) H oxidase in cultured vascular cells. Diabetes 49(11):1939–1945CrossRefPubMed
go back to reference Kasai H (1997) Analysis of a form of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 387:147–163CrossRefPubMed Kasai H (1997) Analysis of a form of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 387:147–163CrossRefPubMed
go back to reference Lind M, Odén A, Fahlén M, Eliasson B (2008) A systematic review of HbA1c variables used in the study of diabetic complications. Diabetes Metab Syndr Clin Res Rev 2:282–293 Lind M, Odén A, Fahlén M, Eliasson B (2008) A systematic review of HbA1c variables used in the study of diabetic complications. Diabetes Metab Syndr Clin Res Rev 2:282–293
go back to reference Makiishi T, Araki S, Koya D, Maeda S, Kashiwagi A, Haneda M (2003) C-106T polymorphism of AKR1B1 is associated with diabetic nephropathy and erythrocyte aldose reductase content in Japanese subjects with type 2 diabetes mellitus. Am J Kidney Dis 42(5):943–951CrossRefPubMed Makiishi T, Araki S, Koya D, Maeda S, Kashiwagi A, Haneda M (2003) C-106T polymorphism of AKR1B1 is associated with diabetic nephropathy and erythrocyte aldose reductase content in Japanese subjects with type 2 diabetes mellitus. Am J Kidney Dis 42(5):943–951CrossRefPubMed
go back to reference Marzouk SA, Zakaria NH, Zeid AAA et al (2014) Role of aldose reductase C-106T polymorphism among diabetic Egyptian patients with different microvascular complications. Am J Exp Clin Res 1(2):18–24 Marzouk SA, Zakaria NH, Zeid AAA et al (2014) Role of aldose reductase C-106T polymorphism among diabetic Egyptian patients with different microvascular complications. Am J Exp Clin Res 1(2):18–24
go back to reference Neamat-Allah M, Feeney SA, Savage DA, Maxwell AP, Hanson RL, Knowler WC, El Nahas AM, Plater ME, Shaw J, Boulton AJ, Duff GW, Cox A (2001) Analysis of the association between diabetic nephropathy and polymorphisms in the aldose reductase gene in type 1 and type 2 diabetes mellitus. Diabet Med 18:906–914CrossRefPubMed Neamat-Allah M, Feeney SA, Savage DA, Maxwell AP, Hanson RL, Knowler WC, El Nahas AM, Plater ME, Shaw J, Boulton AJ, Duff GW, Cox A (2001) Analysis of the association between diabetic nephropathy and polymorphisms in the aldose reductase gene in type 1 and type 2 diabetes mellitus. Diabet Med 18:906–914CrossRefPubMed
go back to reference Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, Yorek MA, Beebe D, Oates PJ, Hammes HP, Giardino I, Brownlee M (2000) Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 404:787–790CrossRefPubMed Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, Yorek MA, Beebe D, Oates PJ, Hammes HP, Giardino I, Brownlee M (2000) Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 404:787–790CrossRefPubMed
go back to reference Olmos P, Bastas MJ, Vollrath V, Toro L, Trincado A, Salinas P, Claro JC, López JM, Acosta AM, Miquel JF, Castro J (2006) C(-106)T polymorphism of the aldose reductase gene and the progression rate of diabetic retinopathy. Diabetes Res Clin Pract 74(2):175–182CrossRefPubMed Olmos P, Bastas MJ, Vollrath V, Toro L, Trincado A, Salinas P, Claro JC, López JM, Acosta AM, Miquel JF, Castro J (2006) C(-106)T polymorphism of the aldose reductase gene and the progression rate of diabetic retinopathy. Diabetes Res Clin Pract 74(2):175–182CrossRefPubMed
go back to reference Pan HZ, Zhang H, Chang D, Li H, Sui H (2008) The change of oxidative stress products in diabetes mellitus and diabetic retinopathy. Br J Ophthalmol 92(4):548–551CrossRefPubMed Pan HZ, Zhang H, Chang D, Li H, Sui H (2008) The change of oxidative stress products in diabetes mellitus and diabetic retinopathy. Br J Ophthalmol 92(4):548–551CrossRefPubMed
go back to reference Pandey KB, Rizvi SI (2010) Resveratrol may protect plasma proteins from oxidation under conditions of oxidative stress in vitro. J Braz Chem Soc 21(5):909–913CrossRef Pandey KB, Rizvi SI (2010) Resveratrol may protect plasma proteins from oxidation under conditions of oxidative stress in vitro. J Braz Chem Soc 21(5):909–913CrossRef
go back to reference Petrash JM (2004) All in the family: aldose reductase and closely related aldo-ketoreductases. Cell Mol Life Sci 61:737–749CrossRefPubMed Petrash JM (2004) All in the family: aldose reductase and closely related aldo-ketoreductases. Cell Mol Life Sci 61:737–749CrossRefPubMed
go back to reference Rema M, Ponnaiya M, Mohan V (1996) Prevalence of retinopathy in non insulin dependent diabetes mellitus at a diabetes centre in Southern India. Diab Res Clin Pract 34:29–36CrossRef Rema M, Ponnaiya M, Mohan V (1996) Prevalence of retinopathy in non insulin dependent diabetes mellitus at a diabetes centre in Southern India. Diab Res Clin Pract 34:29–36CrossRef
go back to reference Rezaee MR, Amiri AA, Hashemi-Soteh MB et al (2015) Aldose reductase C-106T gene polymorphism in type 2 diabetes with microaniopathy in Iranian individuals. Indian J Endocrinol Metab 19(1):95–99 Rezaee MR, Amiri AA, Hashemi-Soteh MB et al (2015) Aldose reductase C-106T gene polymorphism in type 2 diabetes with microaniopathy in Iranian individuals. Indian J Endocrinol Metab 19(1):95–99
go back to reference Serdar M, Sertoglu E, Uyanik M, Tapan S, Akin K, Bilgi C, Kurt I (2012) Comparison of 8-hydroxy-2¢-deoxyguanosine (8-OHdG) levels using mass spectrometer and urine albumin creatinine ratio as a predictor of development of diabetic nephropathy. Free Radic Res 46:1291–1295CrossRefPubMed Serdar M, Sertoglu E, Uyanik M, Tapan S, Akin K, Bilgi C, Kurt I (2012) Comparison of 8-hydroxy-2¢-deoxyguanosine (8-OHdG) levels using mass spectrometer and urine albumin creatinine ratio as a predictor of development of diabetic nephropathy. Free Radic Res 46:1291–1295CrossRefPubMed
go back to reference Shmizu H, Ohtani KI, Tsuchiya T et al (2000) Aldose reductase mRNA expression is associated with rapid development of diabetic microangiopathy in Japanese type 2 diabetic (T2DM) patients. Diabetes Nutr Metab 13:75–79 Shmizu H, Ohtani KI, Tsuchiya T et al (2000) Aldose reductase mRNA expression is associated with rapid development of diabetic microangiopathy in Japanese type 2 diabetic (T2DM) patients. Diabetes Nutr Metab 13:75–79
go back to reference Sivenius K, Aki JP, Partanen J et al (2004) Aldose reductase gene polymorphisms and peripheral nerve function in patients with type 2 diabetes. Diabetes Care 27:9–12CrossRef Sivenius K, Aki JP, Partanen J et al (2004) Aldose reductase gene polymorphisms and peripheral nerve function in patients with type 2 diabetes. Diabetes Care 27:9–12CrossRef
go back to reference Srivastava SK, Ramana KV, Bhatnagar A (2005) Role of aldose reductase and oxidative damage in diabetes and the consequent potential for therapeutic options. Endocr Rev 26(3):380–392 Srivastava SK, Ramana KV, Bhatnagar A (2005) Role of aldose reductase and oxidative damage in diabetes and the consequent potential for therapeutic options. Endocr Rev 26(3):380–392
go back to reference Stratton IM, Adler AI, Neil HA, Matthews DR, Manley SE, Cull CA, Hadden D, Turner RC, Holman RR (2000) Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study. BMJ 321(7258):405–412CrossRefPubMedPubMedCentral Stratton IM, Adler AI, Neil HA, Matthews DR, Manley SE, Cull CA, Hadden D, Turner RC, Holman RR (2000) Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study. BMJ 321(7258):405–412CrossRefPubMedPubMedCentral
go back to reference Tapp RJ, Shaw JE, Zimmet PZ, Balkau B, Chadban SJ, Tonkin AM, Welborn TA, Atkins RC (2004) Albuminuria is evident in the early stages of diabetes onset: results from the Australian Diabetes, Obesity, and Lifestyle study. Am J Kidney Dis 44(5):792–798CrossRefPubMed Tapp RJ, Shaw JE, Zimmet PZ, Balkau B, Chadban SJ, Tonkin AM, Welborn TA, Atkins RC (2004) Albuminuria is evident in the early stages of diabetes onset: results from the Australian Diabetes, Obesity, and Lifestyle study. Am J Kidney Dis 44(5):792–798CrossRefPubMed
go back to reference Tong Y, Lin Y, Zhang Y, Yang J, Zhang Y, Liu H, Zhangand B (2009) Association between TCF7L2 gene polymorphisms and susceptibility to type 2 diabetes mellitus: a large human genome epidemiology (HuGE) review and meta-analysis. BMC Med Genet 10:15CrossRefPubMedPubMedCentral Tong Y, Lin Y, Zhang Y, Yang J, Zhang Y, Liu H, Zhangand B (2009) Association between TCF7L2 gene polymorphisms and susceptibility to type 2 diabetes mellitus: a large human genome epidemiology (HuGE) review and meta-analysis. BMC Med Genet 10:15CrossRefPubMedPubMedCentral
go back to reference Valavanidis A, Vlachogianni T, Fiotakis C (2009) 8-hydroxy-2′ -deoxyguanosine (8-OHdG): a critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 27(2):120–139CrossRefPubMed Valavanidis A, Vlachogianni T, Fiotakis C (2009) 8-hydroxy-2′ -deoxyguanosine (8-OHdG): a critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 27(2):120–139CrossRefPubMed
go back to reference Vimaleswaran KS, Loos RJ (2010) Progress in the genetics of common obesity and type 2 diabetes. Expert Rev Mol Med 12:e7CrossRefPubMed Vimaleswaran KS, Loos RJ (2010) Progress in the genetics of common obesity and type 2 diabetes. Expert Rev Mol Med 12:e7CrossRefPubMed
go back to reference Wang K, Bohren KM, Gabbay KH (1993) Characterization of human aldose reductase gene promoter. J Biol Chem 268:16052–16058PubMed Wang K, Bohren KM, Gabbay KH (1993) Characterization of human aldose reductase gene promoter. J Biol Chem 268:16052–16058PubMed
go back to reference Watarai A, Nakashima E, Hamada Y et al (2006) Aldose reductase gene is associated with diabetic macroangiopathy in Japanese type 2 diabetic patients. Diabet Med 23:894–899CrossRefPubMedPubMedCentral Watarai A, Nakashima E, Hamada Y et al (2006) Aldose reductase gene is associated with diabetic macroangiopathy in Japanese type 2 diabetic patients. Diabet Med 23:894–899CrossRefPubMedPubMedCentral
go back to reference Witzel II, Jelinek HF, Khalaf K, Lee S, Khandoker AH, Alsafar H (2015) Identifying common genetic risk factors of diabetic neuropathies. Front Endocrinol (Lausanne) 6:88 Witzel II, Jelinek HF, Khalaf K, Lee S, Khandoker AH, Alsafar H (2015) Identifying common genetic risk factors of diabetic neuropathies. Front Endocrinol (Lausanne) 6:88
Metadata
Title
Serum 8-hydroxydeoxyguanosine and aldose reductase C-106T polymorphism in type 2 diabetes mellitus and its relation to complications in Egyptian patients
Authors
Mostafa Mohamed Mansour
Mona Abo Bakr El-Hussiny
Nawal Abd El-Galeel Ghareeb
Hamdy Fouad Ali Marzouk
Nabil Ismael Lumon
Publication date
01-01-2018
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 1/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-017-2558-4

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