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01-01-2017 | Original Article

Molecular investigation of Anaplasma and Ehrlichia natural infections in the dromedary camel (Camelus dromedarius) in Iran

Authors: Hassan Sharifiyazdi, Sanaz Jafari, Mohsen Ghane, Saeed Nazifi, Ahmadreza Sanati

Published in: Comparative Clinical Pathology | Issue 1/2017

Abstract

Molecular investigation of Anaplasmataceae species in the dromedary camel (Camelus dromedarius) is still lacking in Iran. In this study, the presence of Anaplasma and Ehrlichia species was investigated in a total of 100 camels in southern Iran. A total number of six camels (6.0 %) were infected by Candidatus Anaplasma camelii genetically related to Anaplasma platys. However, Ehrlichia spp. were not detected in any of the examined dromedary camels. Genetic analysis of the 16S rDNA gene of Iranian strain showed 100 % identity to Anaplasma strains previously reported from Tunisian and Saudi Arabian dromedary camels (accession nos. KM401907 and KF843825, respectively). Furthermore, phylogenetic studies based on the 16S rDNA gene provided more evidence for the circulation of a host-adapted Anaplasma species closely related to A. platys in dromedary camels. In this study, Hyalomma dromedarii were recovered from positive camels in studied regions. These findings highlight the role of this tick species as a potential vector for camel Anaplasmosis. This study describes the first molecular prevalence of Candidatus A. camelii in Iranian dromedary camels.

Arraga-Alvarado CM, Qurollo BA, Parra OC, Berrueta MA, Hegarty BC, Breitschwerdt EB (2014) Molecular evidence of Anaplasma platys infection in two women from Venezuela. AmJTrop Med Hyg 91:1161–1165CrossRef

Bastos AD, Mohammed OB, Bennett NC, Petevinos C, Alagaili AN (2015) Molecular detection of novel Anaplasmataceae closely related to Anaplasma platys and Ehrlichia canis in the dromedary camel (Camelus dromedarius). Vet Mic 179:310–314CrossRef

Belkahia H, Said MB, Sayahi L, Alberti A, Messadi L (2015) Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius). J Infect Dev Ctries 9:1117–1125CrossRefPubMed

Doudier B, Olano J, Parola P, Brouqui P (2010) Factors contributing to emergence of Ehrlichia and Anaplasma spp. as human pathogens. Vet Parasitol 167:149–154CrossRefPubMed

Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR (2001) Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 51:2145–2165CrossRefPubMed

Fard SRN, Fathi S, Asl EN, Nazhad HA, Kazeroni SS (2012) Hard ticks on one-humped camel (Camelus dromedarius) and their seasonal population dynamics in southeast, Iran. Trop Anim Health Prod 44:197–200CrossRefPubMed

Gharbi M, Moussi N, Jedidi M, Mhadhbi M, Sassi L, Darghouth MA (2013) Population dynamics of ticks infesting the one-humped camel (Camelus dromedarius) in Central Tunisia. Ticks Tick Borne Dis 4:488–491CrossRefPubMed

Harvey JW, Simpson CF, Gaskin JM (1987) Cyclic thrombocytopenia induced by a Rickettsi-like agent in dogs. J Infect Dis 137:182–188CrossRef

Kontos VI, Papadopoulos O, French TW (1991) Natural and experimental canine infections with a Greek strain of Ehrlichia platys. Vet Clin Path 20:101–105CrossRef

Li Y, Yang J, Chen Z, Qin G, Li Y, Li Q, Liu J, Liu Z, Guan G, Yin H, Luo J (2015) Anaplasma infection of Bactrian camels (Camelus bactrianus) and ticks in Xinjiang. China Parasit Vectors 8:1CrossRef

Maggi RG, Mascarelli PE, Havenga LN, Naidoo V, Breitschwerdt ED (2013) Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasites Vect 6:103CrossRef

McQuiston JH, McCall CL, Nicholson WL (2003) Ehrlichiosis and related infections. J Am Vet Med Assoc 223:1750–1756CrossRefPubMed

Mentaberre G, Gutiérrez C, Rodríguez NF, Joseph S, González-Barrio D, Cabezón O, de la Fuente J, Gortazar C, Boadella M (2013) A transversal study on antibodies against selected pathogens in Dromedary camels in the Canary Islands, Spain. Vet Microbiol 167:468–473CrossRefPubMed

Nicholson WL, Allen KE, McQuiston JH, Breitschwerdt EB, Little SE (2010) The increasing recognition of rickettsial pathogens in dogs and people. Trend Parasitol 26:205–212CrossRef

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentral

Walker AR, Bouattour A, Camicas JL, Estrada-Pena A, Horak IG, Latif AA, Pegram RG, Prestom PM (2013) Ticks of domestic animals in Africa: A guide to identification of species, Part 4: species of ticks. 2nd edn, Bioscience reports. pp 45–221

Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogentic study. J Bacteriol 173:697–703CrossRefPubMedPubMedCentral

Wernery U, Kaaden OR (2002) Infectious diseases of camelids, 2nd edn. Blackwell Science, Berlin, pp. 23–373CrossRef

Woldehiwet Z (2010) The natural history of Anaplasma phagocytophilum. Vet Parasitol 167:108–122CrossRefPubMed

Ybañez AP, Perez ZO, Gabotero SR, Yandug RT, Kotaro M, Inokuma H (2012) First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu, Philippines. Ticks Tick Borne Dis 3:288–293CrossRefPubMed

Zeleke M, Bekele T (2004) Species of ticks on camels and their seasonal population dynamics in eastern Ethiopia. Trop Anim Health Prod 36:225–231CrossRefPubMed

Title: Molecular investigation of Anaplasma and Ehrlichia natural infections in the dromedary camel (Camelus dromedarius) in Iran
Authors: Hassan Sharifiyazdi
Sanaz Jafari
Mohsen Ghane
Saeed Nazifi
Ahmadreza Sanati
Publication date: 01-01-2017
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 1/2017
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-016-2350-x

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Molecular investigation of Anaplasma and Ehrlichia natural infections in the dromedary camel (Camelus dromedarius) in Iran

Abstract

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Abstract

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