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01-11-2015 | Original Article

Antioxidant, hypolipidemic, and anti-angiotensin-1-converting enzyme properties of lemon (Citrus limon) and lime (Citrus aurantifolia) juices

Authors: Ganiyu Oboh, Fatai O. Bello, Ayokunle O. Ademosun, Ayodele J. Akinyemi, Taiwo M. Adewuni

Published in: Comparative Clinical Pathology | Issue 6/2015

Abstract

Lemon (Citrus limon) and lime (Citrus aurantifolia) juices are used in folk medicine for the management of hypertension and other cardiovascular diseases, but the mechanism of action by which they exert their therapeutic action is unclear. The aim of this study is to investigate the effect of lemon and lime juices on angiotensin-1-converting enzyme (ACE) activity in vitro and investigate the hypocholesterolemic properties of the juices in a high-cholesterol diet rat model. The phenolic content and antioxidant properties of the manually squeezed juices were also determined. Lemon juice had higher total phenol content (64.5 mg/l), while lime juice had higher total flavonoid content (29.5 mg/l). Both juices inhibited ACE activity in a dose-dependent manner and also exhibited antioxidant activities as typified by their ferric reducing power, and radicals (DPPH·, ABTS·, OH·, and NO·) scavenging abilities, as well as inhibition of Fe²⁺- and sodium nitroprusside-induced lipid peroxidation in rat’s liver in vitro. Administration of the juices to rats fed with a high-cholesterol diet caused a significant reduction in plasma total cholesterol, triglyceride, and LDL-cholesterol levels and an increase in plasma HDL-cholesterol levels. The inhibition of ACE activity in vitro and in vivo hypocholesterolemic effect of the juices could explain the use of the juices in the management of cardiovascular diseases.

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Title: Antioxidant, hypolipidemic, and anti-angiotensin-1-converting enzyme properties of lemon (Citrus limon) and lime (Citrus aurantifolia) juices
Authors: Ganiyu Oboh
Fatai O. Bello
Ayokunle O. Ademosun
Ayodele J. Akinyemi
Taiwo M. Adewuni
Publication date: 01-11-2015
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 6/2015
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-015-2088-x

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Antioxidant, hypolipidemic, and anti-angiotensin-1-converting enzyme properties of lemon (Citrus limon) and lime (Citrus aurantifolia) juices

Abstract

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