Skip to main content
Top
Published in: Comparative Clinical Pathology 4/2007

01-11-2007 | Original Article

Oxidative stress and antioxidant enzyme activities in patients with Hashimoto’s thyroiditis

Authors: Julieta Gerenova, Veselina Gadjeva

Published in: Comparative Clinical Pathology | Issue 4/2007

Login to get access

Abstract

We investigated the parameters of oxidative stress in 71 Hashimoto’s thyroiditis patients. They were divided into three sub-groups according to the thyroid function: group I—euthyroid subjects; group II—hypothyroid subjects; and group III—subjects treated with Levothyroxin. Thirty healthy subjects were studied as controls. The level of lipid peroxidation (malondialdehyde, MDA) in the plasma and the antioxidant defences such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities in erythrocytes were measured. Concentrations of MDA and SOD activity were not different in sub-groups of patients and controls. CAT activity was significantly lower in group II in comparison with both controls (p = 0.01) and group III (p = 0.02) as well as in group I compared to the controls (p = 0.04). Activity of GPX in erythrocytes in hypothyroidism was significantly higher compared to controls (p = 0.02). GPX activity in both groups I and III tended to be lower in comparison with controls. Our results indicate a deficiency of cellular antioxidative defense in Hashimoto’s thyroiditis patients in all stages of disease. Accordingly, we suppose that the supplementation with antioxidants from an early stage of the disease, in addition to thyroid hormone replacement, may have a positive benefit in the treatment.
Literature
go back to reference Aksoy DY, Kerimoglu U, Okur H, Canpinar H, Karaagauglu E, Yetgin S et al (2005) Effects of prophylactic thyroid hormone replacement in euthyroid Hashimoto’s thyroiditis. Endocr J 52:337–343PubMedCrossRef Aksoy DY, Kerimoglu U, Okur H, Canpinar H, Karaagauglu E, Yetgin S et al (2005) Effects of prophylactic thyroid hormone replacement in euthyroid Hashimoto’s thyroiditis. Endocr J 52:337–343PubMedCrossRef
go back to reference Alvarado C, Alvarez P, Jimenez L, De la Fuente M (2006) Oxidative stress in leukocytes from young prematurely aging mice is reversed by supplementation with biscuits rich in antioxidants. Dev Comp Immunol 30:1168–1180PubMedCrossRef Alvarado C, Alvarez P, Jimenez L, De la Fuente M (2006) Oxidative stress in leukocytes from young prematurely aging mice is reversed by supplementation with biscuits rich in antioxidants. Dev Comp Immunol 30:1168–1180PubMedCrossRef
go back to reference Amino N, Hagan SR, Yamada N, Refetoff HS (1976) Measurement of circulating thyroid microsomal antibodies by the tanned red cell haemagglutination technique: its usefulness in the diagnosis of autoimmune thyroid disease. Clin Endocrinol 5:115–125 Amino N, Hagan SR, Yamada N, Refetoff HS (1976) Measurement of circulating thyroid microsomal antibodies by the tanned red cell haemagglutination technique: its usefulness in the diagnosis of autoimmune thyroid disease. Clin Endocrinol 5:115–125
go back to reference Beers R, Sizer T (1952) Spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–138PubMed Beers R, Sizer T (1952) Spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–138PubMed
go back to reference Björkman U, Ekholm R (1995) Hydrogen peroxide degradation and glutathione peroxidase activity in cultures of thyroid cells. Mol Cell Endocrinol 111:99–107PubMedCrossRef Björkman U, Ekholm R (1995) Hydrogen peroxide degradation and glutathione peroxidase activity in cultures of thyroid cells. Mol Cell Endocrinol 111:99–107PubMedCrossRef
go back to reference Cadet J, Delatour T, Douki T, Gasparutto D, Pouget JP, Ravanat JL et al (1999) Hydroxyl radicals and DNA base damage. Mutat Res 424:9–21PubMed Cadet J, Delatour T, Douki T, Gasparutto D, Pouget JP, Ravanat JL et al (1999) Hydroxyl radicals and DNA base damage. Mutat Res 424:9–21PubMed
go back to reference Das K, Chainy GB (2001) Modulation of rat liver mitochondrial antioxidant defense system by thyroid hormone. Biochim Biophys Acta 27:1–13 Das K, Chainy GB (2001) Modulation of rat liver mitochondrial antioxidant defense system by thyroid hormone. Biochim Biophys Acta 27:1–13
go back to reference Davies TF, Amino N (1993) A new classification for human autoimmune thyroid disease. Thyroid 3:331–333PubMedCrossRef Davies TF, Amino N (1993) A new classification for human autoimmune thyroid disease. Thyroid 3:331–333PubMedCrossRef
go back to reference Demelash A, Kalsson J-O, Nilsson M, Björkman US (2004) Selenium has a protective role in caspase-3-dependent apoptosis induced by H2O2 in primary cultured pig thyrocytes. Eur J Endocrinol 150:841–849PubMedCrossRef Demelash A, Kalsson J-O, Nilsson M, Björkman US (2004) Selenium has a protective role in caspase-3-dependent apoptosis induced by H2O2 in primary cultured pig thyrocytes. Eur J Endocrinol 150:841–849PubMedCrossRef
go back to reference Duntas LH, Mantzou E, Koutras DA (2003) Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis. Eur J Endocrinol 148:389–393PubMedCrossRef Duntas LH, Mantzou E, Koutras DA (2003) Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis. Eur J Endocrinol 148:389–393PubMedCrossRef
go back to reference Fayadat L, Niccoli-Sire P, Lanet J, Franc J-L (1999) Role of heme in intracellular trafficking of thyroperoxidase and involvement of H2O2 generated at the apical surface of thyroid cells in autocatalytic covalent heme binding. J Biol Chem 274:10533–10558PubMedCrossRef Fayadat L, Niccoli-Sire P, Lanet J, Franc J-L (1999) Role of heme in intracellular trafficking of thyroperoxidase and involvement of H2O2 generated at the apical surface of thyroid cells in autocatalytic covalent heme binding. J Biol Chem 274:10533–10558PubMedCrossRef
go back to reference Gamaley IA, Klyubin IV (1999) Roles of reactive oxygen species: signaling and regulation of cellular functions. Intern Rev Cytol 188:203–255 Gamaley IA, Klyubin IV (1999) Roles of reactive oxygen species: signaling and regulation of cellular functions. Intern Rev Cytol 188:203–255
go back to reference Gärtner R, Gasnier BCH, Dietrich W, Krebs B, Angstwurm MWA (2002) Selenium supplementation in patients with autoimmune thyroiditis decrease thyroid peroxidase antibodies concentrations. J Clin Endocrinol Metab 87:1687–1691PubMedCrossRef Gärtner R, Gasnier BCH, Dietrich W, Krebs B, Angstwurm MWA (2002) Selenium supplementation in patients with autoimmune thyroiditis decrease thyroid peroxidase antibodies concentrations. J Clin Endocrinol Metab 87:1687–1691PubMedCrossRef
go back to reference Gerenova J, Gadjeva V (2006) Influence of methimazole treatment on parameters of oxidative stress in patients with Graves’ disease. Comp Clin Pathol 15:49–54CrossRef Gerenova J, Gadjeva V (2006) Influence of methimazole treatment on parameters of oxidative stress in patients with Graves’ disease. Comp Clin Pathol 15:49–54CrossRef
go back to reference Hammond LJ, Lowdell MW, Cerrano PG, Goode AW, Bottazzo GF, Mirakian R (1997) Analysis of apoptosis in relation to tissue destruction associated with Hashimoto’s autoimmune thyroiditis. J Pathol 182:138–144PubMedCrossRef Hammond LJ, Lowdell MW, Cerrano PG, Goode AW, Bottazzo GF, Mirakian R (1997) Analysis of apoptosis in relation to tissue destruction associated with Hashimoto’s autoimmune thyroiditis. J Pathol 182:138–144PubMedCrossRef
go back to reference Hampton MB, Orrenius S (1997) Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett 414:552–556PubMedCrossRef Hampton MB, Orrenius S (1997) Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett 414:552–556PubMedCrossRef
go back to reference Hayashi N, Tamaki N, Konishi J et al (1986) Sonography of Hashimoto’s thyroiditis. J Clin Ultrasound 14:123–126PubMedCrossRef Hayashi N, Tamaki N, Konishi J et al (1986) Sonography of Hashimoto’s thyroiditis. J Clin Ultrasound 14:123–126PubMedCrossRef
go back to reference Howie AF, Arthur JR, Nicol F, Walker SW, Beech SG, Beckett GJ (1997) Identification of a 57-kilodalton selenoprotein in human thyrocytes as thioredoxin reductase and evidence that its expression is regulated through the calcium-phosphoinositol signaling. J Clin Endocrinol Metab 83:2052–2058CrossRef Howie AF, Arthur JR, Nicol F, Walker SW, Beech SG, Beckett GJ (1997) Identification of a 57-kilodalton selenoprotein in human thyrocytes as thioredoxin reductase and evidence that its expression is regulated through the calcium-phosphoinositol signaling. J Clin Endocrinol Metab 83:2052–2058CrossRef
go back to reference Komosinska-Vassev K, Olczyk K, Kucharz EJ, Marcisz C, Winsz-Szczotka K, Kotulska A (2000) Free radical activity and antioxidant defense mechanisms in patients with hyperthyroidism due to Graves’ disease during therapy. Clin Chim Acta 300:107–117PubMedCrossRef Komosinska-Vassev K, Olczyk K, Kucharz EJ, Marcisz C, Winsz-Szczotka K, Kotulska A (2000) Free radical activity and antioxidant defense mechanisms in patients with hyperthyroidism due to Graves’ disease during therapy. Clin Chim Acta 300:107–117PubMedCrossRef
go back to reference Kotani T, Aratake Y, Hirai K, Fukazawa Y, Sato H, Ohtaki S (1995) Apoptosis in thyroid tissue from patients with Hashimoto’s thyroiditis. Autoimmunity 20:231–236PubMed Kotani T, Aratake Y, Hirai K, Fukazawa Y, Sato H, Ohtaki S (1995) Apoptosis in thyroid tissue from patients with Hashimoto’s thyroiditis. Autoimmunity 20:231–236PubMed
go back to reference Kraiem Z (1998) The measurement of antithyroid autoantibodies in the diagnosis and management of thyroid autoimmune disease. Clin Rev Allergy Immunol 16:219–225PubMed Kraiem Z (1998) The measurement of antithyroid autoantibodies in the diagnosis and management of thyroid autoimmune disease. Clin Rev Allergy Immunol 16:219–225PubMed
go back to reference Mahoney JJ, Vreman HJ, Stevenson DK, Van Kessel AL (1993) Measurement of carboxyhemoglobin and total hemoglobin by five specialized spectrophotometers (CO-oximeters) in comparison with reference methods. Clin Chem 39:1693–1700PubMed Mahoney JJ, Vreman HJ, Stevenson DK, Van Kessel AL (1993) Measurement of carboxyhemoglobin and total hemoglobin by five specialized spectrophotometers (CO-oximeters) in comparison with reference methods. Clin Chem 39:1693–1700PubMed
go back to reference Okayasu I, Saegusa M, Fujiwara M, Hara Y, Rose NR (1995) Enhanced cellular proliferative activity and cell death in chronic thyroiditis and thyroid papillary carcinoma. J Cancer Res Clin Oncol 121:746–752PubMedCrossRef Okayasu I, Saegusa M, Fujiwara M, Hara Y, Rose NR (1995) Enhanced cellular proliferative activity and cell death in chronic thyroiditis and thyroid papillary carcinoma. J Cancer Res Clin Oncol 121:746–752PubMedCrossRef
go back to reference Oziol L, Faure P, Vergely C, Rochette L, Artur Y, Chomard P (2001) In vitro free radical scavenging capacity of thyroid hormones and structural analogues. J Endocrinol 170:197–206PubMedCrossRef Oziol L, Faure P, Vergely C, Rochette L, Artur Y, Chomard P (2001) In vitro free radical scavenging capacity of thyroid hormones and structural analogues. J Endocrinol 170:197–206PubMedCrossRef
go back to reference Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–170PubMed Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–170PubMed
go back to reference Plaser ZA, Cushman LL, Jonson BC (1966) Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal Biochem 16:359–364CrossRef Plaser ZA, Cushman LL, Jonson BC (1966) Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal Biochem 16:359–364CrossRef
go back to reference Resch U, Helsel G, Tatzber F, Sinzinger H (2002) Antioxidant status in thyroid disfunction. Clin Chem Lab Med 40:1132–1134PubMedCrossRef Resch U, Helsel G, Tatzber F, Sinzinger H (2002) Antioxidant status in thyroid disfunction. Clin Chem Lab Med 40:1132–1134PubMedCrossRef
go back to reference Rhee SG (1999) Redox signaling: hydrogen peroxide as intracellular messenger. Exp Mol Med 31:53–59PubMed Rhee SG (1999) Redox signaling: hydrogen peroxide as intracellular messenger. Exp Mol Med 31:53–59PubMed
go back to reference Riou C, Remy C, Rabilloud R, Rousset B, Fonlupt P (1998) H2O2 induces apoptoses of pig thyrocytes in culture. J Endocrinol 156:315–322PubMedCrossRef Riou C, Remy C, Rabilloud R, Rousset B, Fonlupt P (1998) H2O2 induces apoptoses of pig thyrocytes in culture. J Endocrinol 156:315–322PubMedCrossRef
go back to reference Sadani GR, Nadkarni GD (1996) Role of tissue antioxidant defense in thyroid cancers. Cancer Lett 109:231–235PubMedCrossRef Sadani GR, Nadkarni GD (1996) Role of tissue antioxidant defense in thyroid cancers. Cancer Lett 109:231–235PubMedCrossRef
go back to reference Sugawara M, Kita T, Lee ED, Takamatsu J, Hagen JA, Kuma K et al (1998) Deficiency of thyroid superoxide dismutase in endemic goiter tissue. J Clin Endocrinol Metab 67:1156–1161CrossRef Sugawara M, Kita T, Lee ED, Takamatsu J, Hagen JA, Kuma K et al (1998) Deficiency of thyroid superoxide dismutase in endemic goiter tissue. J Clin Endocrinol Metab 67:1156–1161CrossRef
go back to reference Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500PubMed Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500PubMed
go back to reference Tanimoto C, Hirakawa S, Kawasaki H, Hayakawa N, Ota Z (1995) Apoptosis in thyroid diseases: a histochemical study. Endocr J 42:193–201PubMed Tanimoto C, Hirakawa S, Kawasaki H, Hayakawa N, Ota Z (1995) Apoptosis in thyroid diseases: a histochemical study. Endocr J 42:193–201PubMed
Metadata
Title
Oxidative stress and antioxidant enzyme activities in patients with Hashimoto’s thyroiditis
Authors
Julieta Gerenova
Veselina Gadjeva
Publication date
01-11-2007
Publisher
Springer-Verlag
Published in
Comparative Clinical Pathology / Issue 4/2007
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-007-0689-8

Other articles of this Issue 4/2007

Comparative Clinical Pathology 4/2007 Go to the issue