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Journal of Anesthesia
Published in: Journal of Anesthesia 4/2019

01-08-2019 | Cytokines | Original Article

Neuroprotective effects of neurotropin in a mouse model of hypoxic–ischemic brain injury

Authors: Sohei Hishiyama, Masakazu Kotoda, Tadahiko Ishiyama, Kazuha Mitsui, Takashi Matsukawa

Published in: Journal of Anesthesia | Issue 4/2019

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Abstract

Purpose

Ischemic–hypoxic insult leads to detrimental effects on multiple organs. The brain is especially vulnerable, and it is hard to regenerate once damaged. Currently, therapeutic options are very limited. Previous studies have reported neuroprotective effects of neurotropin, a non-protein extract derived from the inflamed skin of rabbits inoculated with vaccinia virus, using a murine model of peripheral nerve injury and cultured cell lines. However, whether neurotropin might have protective effects against brain injuries remains unclear. We, therefore, investigated the neuroprotective effect of neurotropin and possible underlying mechanisms, using a mouse model of hypoxic–ischemic brain injury.

Methods

Hypoxic–ischemic brain injury was induced via a combination of the left common carotid artery occlusion and exposure to hypoxic environment (8% oxygen) in adult male C57BL/6 mice. Immediately following induction of hypoxia–ischemia, mice received either saline or 2.4 units of neurotropin. The survival rate, neurological function, infarct volume, and expression of inflammatory cytokines were evaluated.

Results

Compared to the control group, the neurotropin group exhibited a significantly higher survival rate (100% vs. 62.5%, p < 0.05) and lower neurological deficit scores (1; 0–2 vs. 3; 0–5, median; range, p < 0.05) after the hypoxic–ischemic insult. The administration of neurotropin also reduced infarct volume (18.3 ± 5.1% vs. 38.3 ± 7.2%, p < 0.05) and mRNA expression of pro-inflammatory cytokines.

Conclusions

The post-treatment with neurotropin improved survival and neurological outcomes after hypoxic–ischemic insult. Our results indicate that neurotropin has neuroprotective effects against hypoxic–ischemic brain injury by suppressing pro-inflammatory cytokines.
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Metadata
Title
Neuroprotective effects of neurotropin in a mouse model of hypoxic–ischemic brain injury
Authors
Sohei Hishiyama
Masakazu Kotoda
Tadahiko Ishiyama
Kazuha Mitsui
Takashi Matsukawa
Publication date
01-08-2019
Publisher
Springer Japan
Published in
Journal of Anesthesia / Issue 4/2019
Print ISSN: 0913-8668
Electronic ISSN: 1438-8359
DOI
https://doi.org/10.1007/s00540-019-02655-z

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