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Journal of Anesthesia
Published in: Journal of Anesthesia 5/2011

01-10-2011 | Original Article

Effects of propofol and pentobarbital on calcium concentration in presynaptic boutons on a rat hippocampal neuron

Authors: Shinichi Ito, Hitomi Sugiyama, Seiko Kitahara, Yoshimi Ikemoto, Takeshi Yokoyama

Published in: Journal of Anesthesia | Issue 5/2011

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Abstract

Purpose

Numerous reports suggest that intravenously administered (IV) anesthetics affect postsynaptic events in the central nervous system. However, there is little evidence about how general anesthetics influence the presynaptic processes. The level of presynaptic calcium (Ca2+) concentration ([Ca2+]pre) regulates neurotransmitter release. In this study, we investigated the effects of anesthetic propofol IV and the barbiturate pentobarbital on neurotransmitter release by measuring [Ca2+]pre in the presynaptic nerve terminals (boutons) on a dissociated single hippocampal rat neuron.

Methods

Sprague-Dawley rats 10–14 days old were decapitated under pentobarbital anesthesia, and brain slices were prepared. The hippocampal CA1 area was touched with a fire-polished glass pipette, which vibrated horizontally, and neurons were dissociated, along with the attached presynaptic boutons. The presynaptic boutons were visualized under a confocal laser-scanning microscope after staining with FM1-43 dye, and [Ca2+]pre was measured with acetoxymethyl ester of fluo-3 (fluo-3 AM).

Results

High potassium (K+) (15–90 mM) increased the [Ca2+]pre in the Ca2+-containing solution in a concentration-dependent manner. Whereas propofol (10 μM) and pentobarbital (300 μM) suppressed the high K+ (60 mM)-induced increase in [Ca2+]pre in the boutons attached to the dendrite, they did not affect [Ca2+]pre in the boutons attached to the soma or dendrite base. As a large majority of excitatory synapses are located on dendritic spines, these agents may affect Ca2+ mobilization in the excitatory presynaptic boutons.

Conclusions

Propofol and pentobarbital may affect neurotransmitter release from the excitatory presynaptic nerve terminals due to inhibition of increase in [Ca2+]pre.
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Metadata
Title
Effects of propofol and pentobarbital on calcium concentration in presynaptic boutons on a rat hippocampal neuron
Authors
Shinichi Ito
Hitomi Sugiyama
Seiko Kitahara
Yoshimi Ikemoto
Takeshi Yokoyama
Publication date
01-10-2011
Publisher
Springer Japan
Published in
Journal of Anesthesia / Issue 5/2011
Print ISSN: 0913-8668
Electronic ISSN: 1438-8359
DOI
https://doi.org/10.1007/s00540-011-1186-4

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