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Supportive Care in Cancer
Published in: Supportive Care in Cancer 10/2017

01-10-2017 | Original Article

Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil

Authors: Leticia Helena Theodoro, Mariéllen Longo, Vivian Cristina Noronha Novaes, Daniela Maria Janjacomo Miessi, Marcio Luiz Ferro-Alves, Edilson Ervolino, Juliano Milanezi de Almeida, Valdir Gouveia Garcia

Published in: Supportive Care in Cancer | Issue 10/2017

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Abstract

Purpose

The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy.

Methods

EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically.

Results

There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p < 0.05). The immunohistochemical analysis showed no significant difference for TRAP and osteoprotegerin, but lower RANKL immunolabeling was observed in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups compared with the 5FU group at 15 days. There was lower TNF-α and IL-6 immunolabeling in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups and higher IL-10 immunolabeling in 5FU/SRP/aPDT at 30 days.

Conclusion

LLLT and aPDT adjuvant to SRP minimized the effects of 5-FU on periodontal disease. Furthermore, aPDT promoted greater benefits in bone loss control and inflammatory response.
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Metadata
Title
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil
Authors
Leticia Helena Theodoro
Mariéllen Longo
Vivian Cristina Noronha Novaes
Daniela Maria Janjacomo Miessi
Marcio Luiz Ferro-Alves
Edilson Ervolino
Juliano Milanezi de Almeida
Valdir Gouveia Garcia
Publication date
01-10-2017
Publisher
Springer Berlin Heidelberg
Published in
Supportive Care in Cancer / Issue 10/2017
Print ISSN: 0941-4355
Electronic ISSN: 1433-7339
DOI
https://doi.org/10.1007/s00520-017-3738-0

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