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01-11-2012 | Original Article

Urinary NGAL, cystatin C, β2-microglobulin, and osteopontin significance in hydronephrotic children

Authors: Mia Gebauer Madsen, Rikke Nørregaard, Johan Palmfeldt, Lars Henning Olsen, Jørgen Frøkiær, Troels Munch Jørgensen

Published in: Pediatric Nephrology | Issue 11/2012

Abstract

Background

Ureteropelvic junction obstruction (UPJO) accounts for 35 % of all congenital hydronephrosis cases. The challenge in managing childhood hydronephrosis is to distinguish obstructive from nonobstructive cases and, thereby, identify patients requiring surgical intervention. This study aimed to examine four urinary proteins as potential biomarkers of obstruction in hydronephrosis.

Methods

Urine samples from 24 children with UPJO were collected pre-, peri-, and postoperatively, together with urine samples from healthy children. Neutrophil gelatinase-associated lipocalin (NGAL), cystatin C (CyC), β2-microglobulin (β2-M), and osteopontin (OPN) in the samples were measured simultaneously using multiplex sandwich immunoassay technology.

Results

Compared with controls, NGAL and β2-M were significantly increased in urine from patients with obstructed kidneys at the time of surgery. This increase was followed by a decrease and stabilization to the same level as that of the controls. Furthermore, age was negatively correlated with preoperative urinary concentrations of CyC, β2-M, and OPN.

Conclusions

This study confirms increased concentrations of NGAL and β2-M in urine from obstructed kidneys and should be tested in larger studies to ascertain their ability to identify obstruction and to determine the importance of age-adjusted reference values.

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Title: Urinary NGAL, cystatin C, β2-microglobulin, and osteopontin significance in hydronephrotic children
Authors: Mia Gebauer Madsen
Rikke Nørregaard
Johan Palmfeldt
Lars Henning Olsen
Jørgen Frøkiær
Troels Munch Jørgensen
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: Pediatric Nephrology / Issue 11/2012
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-012-2217-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Urinary NGAL, cystatin C, β2-microglobulin, and osteopontin significance in hydronephrotic children

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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