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01-02-2007 | Original Article

Renal caveolin-1 expression in children with unilateral ureteropelvic junction obstruction

Authors: Patricia G. Vallés, Walter Manucha, Liliana Carrizo, José Vega Perugorria, Alicia Seltzer, Celeste Ruete

Published in: Pediatric Nephrology | Issue 2/2007

Abstract

Caveolae are plasma membrane invaginations that contain a variety of signal transduction molecules and receptors for growth factors and cytokines. This study was performed to examine the in vivo expression and localization of caveolin-1 in kidneys from 19 children who underwent surgery release of ureteropelvic junction obstruction (UPJO) in relation to renal function and degree of tubulointerstitial fibrosis. Renal biopsies were carried out at the time of surgery for obstruction release. Kidney tissue from children of similar age removed because of carcinoma was used as control. Expression of caveolin-1 at the protein level in renal tissue and urine was demonstrated in patients with technetium 99 m labeled diethylene triamine pentaacetate (⁹⁹Tc DTPA) renal scan 28.8±2% and increased tubular interstitial fibrosis in seven patients at the time of obstruction release. Colocalization staining of AT₁ angiotensin II receptor with caveolin-1 in basolateral membrane of epithelial tubule cells, enhanced AT₁ messenger ribonucleic acid (mRNA) and decreased endothelial nitric oxide synthase (eNOS), were shown in these patients. In contrast, absence of association of caveolin-1 with AT₁ receptor expression in proximal and collecting tubule membranes with AT₁ receptor mRNA and eNOS mRNA expression near control were demonstrated in 12 patients, with ⁹⁹Tc DTPA renal scan 39.7+2.1% and no evidence of tubulointerstitial fibrosis. From our results, the role of caveolin-1 as a factor contributing to the severity of the tubulointerstitial process resulting from obstructive nephropathy could be suggested.

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Title: Renal caveolin-1 expression in children with unilateral ureteropelvic junction obstruction
Authors: Patricia G. Vallés
Walter Manucha
Liliana Carrizo
José Vega Perugorria
Alicia Seltzer
Celeste Ruete
Publication date: 01-02-2007
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Nephrology / Issue 2/2007
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-006-0290-4

At a glance: The STEP trials

Springer Medicine

Renal caveolin-1 expression in children with unilateral ureteropelvic junction obstruction

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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