Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Surgical Endoscopy
Published in: Surgical Endoscopy 4/2004

01-04-2004 | Original article

Hematogenous tumor cell dissemination during colonoscopy for colorectal cancer

Authors: M. Koch, P. Kienle, P. Sauer, F. Willeke, K. Buhl, A. Benner, T. Lehnert, C. Herfarth, M. von Knebel Doeberitz, J. Weitz

Published in: Surgical Endoscopy | Issue 4/2004

Login to get access

Abstract

Background: It has long been suspected that mechanical influences may enhance the release of viable colorectal cancer cells into the circulation. The objective of this study was to determine the extent of hematogenous tumor cell spread in colorectal cancer patients during colonoscopy. Methods: Peripheral venous blood samples were taken before and after colonoscopy from 44 patients with colorectal cancer. Blood samples were examined using a reverse-transcriptase polymerase chain reaction assay to amplify cytokeratin 20 transcripts. Results: Eleven patients with colorectal cancer displayed circulating tumor cells before and after colonoscopy (25%), whereas tumor cells were detected in six of 44 patients (14%) only after the procedure (p = 0.03, McNemar’s test: tumor cell detection before after colonoscopy). All control samples consistently tested negative. Conclusions: Mechanical forces may result in enhanced release of viable colorectal cancer cells into the circulation; however, the clinical significance of these results needs to be clarified.
Literature
1.
Agresti, A 1990Categorial data analysis.WileyNew York
2.
Allgayer, H, Heiss, MM, Riesenberg, R,  et al. 1997Urokinase plasminogen activator receptor (uPA-R): one potential characteristic of metastatic phenotypes in minimal residual tumor disease.Cancer Res5713941399PubMed
3.
4.
Barnes, JP 1952Physiologic resection of the right colon.Surg Gynecol Obstet94722726PubMed
5.
Basha, G, Ectors, N, Penninckx, F,  et al. 1997Tumor cell implantation after colonoscopy with biopsies in a patient with rectal cancer.Dis Colon Rectum4015081510PubMed
6.
Brown, DC, Purushotham, AD, Birnie, GD,  et al. 1995Detection of intraoperative tumor cell dissemination in patients with breast cancer by use of reverse transcription and polymerase chain reaction.Surgery11796101
7.
Burchill, SA, Bradbury, MF, Pittman, K,  et al. 1995Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction.Br J Cancer71278281
8.
Chausovsky, G, Luchansky, M, Figer, A,  et al. 1999Expression of cytokeratin 20 in the blood of patients with disseminated carcinoma of the pancreas, colon, stomach, and the lung.Cancer8623982405CrossRefPubMed
9.
Eschwege, P, Dumas, F, Blanchet, P,  et al. 1995Hematogenous dissemination of prostatic epithelial cells during radical prostatectomy.Lancet34615281530CrossRefPubMed
10.
Fleiss, JL 1973Statistical methods for rates and proportions, 2nd edn.WileyNew York
11.
Funaki, NO, Tanaka, J, Itami, A,  et al. 1997Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA.Life Sci60643652CrossRefPubMed
12.
Funaki, NO, Tanaka, J, Ohshio, G,  et al. 1998Cytokeratin 20 mRNA in peripheral venous blood of colorectal carcinoma patients.Br J Cancer7713271332
13.
Ghossein, R, Bhattacharya, S, Rosai, J 1999Molecular detection of micrometastases and circulating tumor cells in solid tumors.Clin Cancer Res519501960PubMed
14.
Glaves, D, Huben, RP, Weis, L 1988Haematogenous dissemination of cells from human renal adenocarcinomas.Br J Cancer573235PubMed
15.
Howard, ML, Greene, FL 1990The effect of preoperative endoscopy on recurrence and survival following surgery for colorectal carcinoma.Am Surg56124127PubMed
16.
Hu, XC, Chow, LW 2000Fine needle aspiration may shed breast cells into peripheral blood as determined by RT-PCR.Oncology59217222CrossRefPubMed
17.
Johnson, PWM, Burchill, SA, Selby, PJ 1995The molecular detection of circulating tumor cells.Br J Cancer72268276PubMed
18.
Jung, R, Petersen, K, Krüger, W,  et al. 1999Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes.Br J Cancer81870873
19.
Koch, M, Weitz, J, Kienle, P,  et al. 2001Comparative analysis of tumor cell dissemination in mesenteric, central and peripheral venous blood in patients with colorectal cancer.Arch Surg1368589PubMed
20.
Koo, J, Fung, K, Siu, KF,  et al. 1983Recovery of malignant tumor cells from the right atrium during hepatic resection for hepatocellular carcinoma.Cancer5219521956PubMed
21.
Macfarlane, DE, Dahle, CE 1993Isolating RNA from whole blood—the dawn of RNA-based diagnosis?Nature362186188CrossRefPubMed
22.
Moll, R, Löwe, A, Laufer, J, Franke, WW 1992Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies.Am J Pathol140427447PubMed
23.
Moll, R, Zimbelmann, R, Goldschmidt, MD,  et al. 1993The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas.Differentiation537593PubMed
24.
Moreno, JG, O’Hara, M, Long, JP,  et al. 1997Transrectal ultrasound-guided biopsy causes hematogenous dissemination of prostate cells as determined by RT-PCR.Urology49515520CrossRefPubMed
25.
Nishizaki, T, Matsumata, T, Kanematsu, T 1990Surgical manipulation of VX2 carcinoma in the rabbit liver evokes enhancement of metastasis.J Surg Res499297PubMed
26.
Pappalardo, G, Polettini, E, Frattaroli, FM,  et al. 2000Magnetic resonance colonography versus conventional colonoscopy for the detection of colonic endoluminal lesions.Gastroenterology119300304PubMed
27.
Rex, DK 1998CT and MR colography (virtual colonoscopy): status report.J Clin Gastroenterol27199203CrossRefPubMed
28.
Scapa, E, Broide, E, Pinhasov, I 1997The effect of colonoscopy on tumor markers.Surg Laparosc Endosc7477479CrossRefPubMed
29.
So, JB, Alexander, DJ, Chia, YW,  et al. 1997A study of the effects of bowel preparation on CEA levels in patients undergoing surveillance colonoscopy.J R Coll Surg Edinburgh42105106
30.
Sobin, LH, Wittekind, C 1997UICC: TNM classification of malignant tumors, 5th edn.WileyLondon
31.
Soeth, E, Vogel, I, Röder, C,  et al. 1997Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR.Cancer Res5731063110
32.
Sollenberger, LL, Eisenstat, TE, Rubin, RJ,  et al. 1988Is preoperative colonoscopy necessary in carcinoma of the colon and rectum?Am Surg54113115PubMed
33.
Sträter, J, Wedding, U, Earth, TF,  et al. 1996Rapid onset of apoptosis in vitro follows disruption of β1-integrin/matrix interactions in human colonic crypt cells.Gastroenterology11017761784PubMed
34.
Turnbull, RB, Kyle, K, Watson, FR,  et al. 1967Cancer of the colon: the influence of the no-touch isolation technique on survival rates.Ann Surg166420425PubMed
35.
Turner, J, Sturgeon, CM 1993Effect of bowel cleansing and colonoscopy on serum CEA levels.J R Coll Surg Edinburgh388991
36.
37.
Weitz, J, Kienle, P, Lacroix, J,  et al. 1998Dissemination of tumor cells in patients undergoing surgery for colorectal cancer.Clin Cancer Res4343348PubMed
38.
Weitz, J, Kienle, P, Magener, A,  et al. 1999Detection of disseminated colorectal cancer cells in lymph nodes, blood and bone marrow.Clin Cancer Res518301836
39.
Weitz, J, Koch, M, Kienle, P,  et al. 2000Detection of hematogenous tumor cell dissemination in patients undergoing resection of liver metastases of colorectal cancer.Ann Surg2326672PubMed
40.
Wharton, RQ, Jonas, SK, Glover, C,  et al. 1999Increased detection of circulating tumor cells in the blood of colorectal carcinoma patiens using two reverse transcription-PCR and multiple blood samples.Clin Cancer Res541584163
41.
Wiggers, T, Jeekel, J, Arends, JW,  et al. 1988No-touch isolation technique in colon cancer: a controlled prospective trial.Br J Surg75409415PubMed
42.
Willeke, F, Ridder, R, Mechtersheimer, G,  et al. 1998Analysis of FUS-CHOP fusion transcripts in different types of soft tissue liposarcoma and their diagnostic implications.Clin Cancer Res417791884PubMed
43.
Wyld, DK, Selby, P, Perren, TJ,  et al. 1998Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytokeratin 20.Int J Cancer79288293CrossRef
44.
Yamaguchi, K, Takagi, Y, Shinichirou, A,  et al. 2000Significant detection of circulating cancer cells in the blood by reverse transcriptase-polymerase chain reaction during colorectal cancer resection.Ann Surg2325865CrossRefPubMed
45.
Yuan, J, Coplen, DE, Petros, JA,  et al. 1992Effects of rectal examination, prostatic massage, ultrasonography and needle biopsy on serum prostate specific antigen levels.J Urol147810814PubMed
Metadata
Title
Hematogenous tumor cell dissemination during colonoscopy for colorectal cancer
Authors
M. Koch
P. Kienle
P. Sauer
F. Willeke
K. Buhl
A. Benner
T. Lehnert
C. Herfarth
M. von Knebel Doeberitz
J. Weitz
Publication date
01-04-2004
Publisher
Springer-Verlag
Published in
Surgical Endoscopy / Issue 4/2004
Print ISSN: 0930-2794
Electronic ISSN: 1432-2218
DOI
https://doi.org/10.1007/s00464-003-9066-0

Other articles of this Issue 4/2004

Surgical Endoscopy 4/2004 Go to the issue

Original article

A mathematical model for preoperative planning of radiofrequency ablation of hepatic tumors

Original article

Modified devascularization surgery for isolated gastric varices assessed by endoscopic ultrasonography

Original article

Partial laparoscopic decapsulation of congenital splenic cysts: a medium-term evaluation proves the efficiency in children

Original article

Magnitude of abdominal incision affects the duration of postoperative ileus in rats

Original article

A technique for real-time digital measurements in laparoscopic surgery

Original article

Prevention of adhesion formation to polypropylene mesh by collagen coating: A randomized controlled study in a rat model of ventral hernia repair