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Published in: Dysphagia 1/2014

01-02-2014 | Original Article

A Novel Animal Model of Dysphagia Following Stroke

Authors: Naoto Sugiyama, Eiji Nishiyama, Yukitoshi Nishikawa, Takashi Sasamura, Shinji Nakade, Katsumasa Okawa, Tadashi Nagasawa, Akane Yuki

Published in: Dysphagia | Issue 1/2014

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Abstract

Patients who have an ischemic stroke are at high risk of swallowing disorders. Aspiration due to swallowing disorders, specifically delayed trigger of the pharyngeal stage of swallowing, predisposes such patients to pneumonia. In the present study, we evaluated swallowing reflex in a rat model of transient middle cerebral artery occlusion (tMCAO), which is one of the most common experimental animal models of cerebral ischemia, in order to develop a novel animal model of dysphagia following ischemic stroke. A swallowing reflex was elicited by a 10-s infusion of distilled water (DW) to the pharyngolaryngeal region in the tMCAO rat model. Swallowing reflex was estimated using the electromyographic activity of the mylohyoid muscle from 1 to 3 weeks after surgery. Two weeks after tMCAO, the number of swallows significantly decreased and the onset latency of the first swallow was prolonged compared with that of the sham group. The number of swallows in rats significantly increased by infusions of 10 mM citric acid and 0.6 μM capsaicin to the pharyngolaryngeal region compared with the number from infusion of DW. It has been reported that sensory stimulation of the pharyngolaryngeal region with citric acid, capsaicin, and l-menthol ameliorates hypofunction of pharyngeal-stage swallowing in dysphagia patients. Therefore, the tMCAO rat model may show some of the symptoms of pharyngeal-stage swallowing disorders, similar to those in patients with ischemic stroke. This rat tMCAO model has the potential to become a novel animal model of dysphagia following stroke that is useful for development of therapeutic methods and drugs.
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Metadata
Title
A Novel Animal Model of Dysphagia Following Stroke
Authors
Naoto Sugiyama
Eiji Nishiyama
Yukitoshi Nishikawa
Takashi Sasamura
Shinji Nakade
Katsumasa Okawa
Tadashi Nagasawa
Akane Yuki
Publication date
01-02-2014
Publisher
Springer US
Published in
Dysphagia / Issue 1/2014
Print ISSN: 0179-051X
Electronic ISSN: 1432-0460
DOI
https://doi.org/10.1007/s00455-013-9481-x

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