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Open Access 01-06-2018 | Original Article

Spatio-temporal specialization of GABAergic septo-hippocampal neurons for rhythmic network activity

Authors: Gunes Unal, Michael G. Crump, Tim J. Viney, Tímea Éltes, Linda Katona, Thomas Klausberger, Peter Somogyi

Published in: Brain Structure and Function | Issue 5/2018

Abstract

Medial septal GABAergic neurons of the basal forebrain innervate the hippocampus and related cortical areas, contributing to the coordination of network activity, such as theta oscillations and sharp wave-ripple events, via a preferential innervation of GABAergic interneurons. Individual medial septal neurons display diverse activity patterns, which may be related to their termination in different cortical areas and/or to the different types of innervated interneurons. To test these hypotheses, we extracellularly recorded and juxtacellularly labeled single medial septal neurons in anesthetized rats in vivo during hippocampal theta and ripple oscillations, traced their axons to distant cortical target areas, and analyzed their postsynaptic interneurons. Medial septal GABAergic neurons exhibiting different hippocampal theta phase preferences and/or sharp wave-ripple related activity terminated in restricted hippocampal regions, and selectively targeted a limited number of interneuron types, as established on the basis of molecular markers. We demonstrate the preferential innervation of bistratified cells in CA1 and of basket cells in CA3 by individual axons. One group of septal neurons was suppressed during sharp wave-ripples, maintained their firing rate across theta and non-theta network states and mainly fired along the descending phase of CA1 theta oscillations. In contrast, neurons that were active during sharp wave-ripples increased their firing significantly during “theta” compared to “non-theta” states, with most firing during the ascending phase of theta oscillations. These results demonstrate that specialized septal GABAergic neurons contribute to the coordination of network activity through parallel, target area- and cell type-selective projections to the hippocampus.

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Title: Spatio-temporal specialization of GABAergic septo-hippocampal neurons for rhythmic network activity
Authors: Gunes Unal
Michael G. Crump
Tim J. Viney
Tímea Éltes
Linda Katona
Thomas Klausberger
Peter Somogyi
Publication date: 01-06-2018
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 5/2018
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-018-1626-0

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Spatio-temporal specialization of GABAergic septo-hippocampal neurons for rhythmic network activity

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