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Open Access 01-09-2008 | Original Article

Three-dimensional organization of dendrites and local axon collaterals of shell and core medium-sized spiny projection neurons of the rat nucleus accumbens

Authors: Yvette C. van Dongen, Philippe Mailly, Anne-Marie Thierry, Henk J. Groenewegen, Jean-Michel Deniau

Published in: Brain Structure and Function | Issue 1-2/2008

Abstract

Medium-sized spiny projection neurons (MSN) in the head of the primate caudate nucleus are thought to have preferred dendritic orientations that tend to parallel the orientations of the striosomes. Moreover, recurrent axon collaterals of MSN in the rat dorsal striatum have been categorized into two types, i.e., restricted and widespread. The nucleus accumbens (Acb) has a highly complex compartmental organization, and the spatial organization of dendritic and axonal arbors of MSN has not yet been systematically studied. In this study, using single-cell juxtacellular labeling with neurobiotin as well as anterograde neuroanatomical tracing with biotinylated dextran amine, we investigated the three-dimensional (3D) organization of dendrites and axons of MSN of the rat Acb in relation to subregional (shell-core) and compartmental (patch-matrix) boundaries. Our results show that dendritic arbors of MSN in both the Acb shell and core subregions are preferentially oriented, i.e., they are flattened in at least one of the 3D-planes. The preferred orientations are influenced by shell-core and patch-matrix boundaries, suggesting parallel and independent processing of information. Dendritic orientations of MSN of the Acb core are more heterogeneous than those of the shell and the dorsal striatum, suggesting a more complex distribution of striatal inputs within the core. Although dendrites respect the shell-core and patch-matrix boundaries, recurrent axon collaterals may cross these boundaries. Finally, different degrees of overlap between dendritic and axonal arborizations of individual MSN were identified, suggesting various possibilities of lateral inhibitory interactions within and between, functionally distinct territories of the Acb.

The anterograde tracer injections and the single-cell juxtacellular injections were performed in two different laboratories; minor differences in the surgical and staining procedures are the result of different traditions in these laboratories, rather than that they represent as essentially different steps in the protocols.

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Title: Three-dimensional organization of dendrites and local axon collaterals of shell and core medium-sized spiny projection neurons of the rat nucleus accumbens
Authors: Yvette C. van Dongen
Philippe Mailly
Anne-Marie Thierry
Henk J. Groenewegen
Jean-Michel Deniau
Publication date: 01-09-2008
Publisher: Springer-Verlag
Published in: Brain Structure and Function / Issue 1-2/2008
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-008-0173-5

At a glance: The STEP trials

Springer Medicine

Three-dimensional organization of dendrites and local axon collaterals of shell and core medium-sized spiny projection neurons of the rat nucleus accumbens

Abstract

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Springer Medicine

Abstract

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