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01-02-2014 | Original Article

Oil Red O-assessed macrosteatosis in liver transplant donor biopsies predicts ischemia–reperfusion injury and clinical outcome

Authors: Henning Reis, Patricia T. Peterek, Jeremias Wohlschlaeger, Gernot M. Kaiser, Zoltan Mathe, Benjamin Juntermanns, Georgios C. Sotiropoulos, Ulrich Beckhove, Ali Canbay, Ulrike Wirges, Andre Scherag, Juergen-Walter Treckmann, Andreas Paul, Hideo Andreas Baba

Published in: Virchows Archiv | Issue 2/2014

Abstract

Steatosis in donor livers is an accepted adverse prognostic factor after liver transplantation. While its semiquantitative assessment shows varying reproducibility, it is questioned as a standard method. Additionally, the influence of hepatic steatosis on ischemia/reperfusion injury (I/R injury) has not been evaluated in biopsies after reperfusion. We compared different staining and analyzing methods for the assessment of donor liver steatosis in order to predict I/R injury and clinical outcome after transplantation. To do this, 56 paired pre- and post-reperfusion liver biopsies were analyzed for macro- (MaS)/micro- (MiS) and total steatosis in cryo and permanent sections by special fat (Oil Red O or ORO) and standard stains. Computerized morphometrical analyses were compared to the semiquantitative assessment by a pathologist. I/R injury was determined histopathologically and by M30 immunohistochemistry. We found ORO to be more sensitive in detecting hepatic steatosis with higher reproducibility for MaS. Semiquantitative analyses were highly reproducible and not inferior to computerized morphometry. Categorized MaS as determined by ORO correlated with the extent of I/R injury, initial poor function, liver enzymes, and survival. Therefore fat stains like ORO are a reliable and easy method comprising significant advantages in the evaluation of hepatic steatosis and are thereby of prognostic value. Computerized analysis is a precise tool though not superior to semiquantitative analyses.

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Title: Oil Red O-assessed macrosteatosis in liver transplant donor biopsies predicts ischemia–reperfusion injury and clinical outcome
Authors: Henning Reis
Patricia T. Peterek
Jeremias Wohlschlaeger
Gernot M. Kaiser
Zoltan Mathe
Benjamin Juntermanns
Georgios C. Sotiropoulos
Ulrich Beckhove
Ali Canbay
Ulrike Wirges
Andre Scherag
Juergen-Walter Treckmann
Andreas Paul
Hideo Andreas Baba
Publication date: 01-02-2014
Publisher: Springer Berlin Heidelberg
Published in: Virchows Archiv / Issue 2/2014
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-013-1512-3

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Oil Red O-assessed macrosteatosis in liver transplant donor biopsies predicts ischemia–reperfusion injury and clinical outcome

Abstract

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Abstract

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