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01-03-2012 | Original Article

SOX9 expression and its methylation status in gastric cancer

Authors: Minhua Sun, Hiroshi Uozaki, Rumi Hino, Akiko Kunita, Aya Shinozaki, Tetsuo Ushiku, Takashi Hibiya, Kimiko Takeshita, Maya Isogai, Kenzo Takada, Masashi Fukayama

Published in: Virchows Archiv | Issue 3/2012

Abstract

SOX9 is a member of the SOX [Sry-related high-mobility group (HMG) box] family and is required for the development and differentiation of multiple cell lineages. To clarify the significance of SOX9 in gastric carcinoma (GC), immunohistochemical expression of SOX9 and the CpG island methylation status of SOX9 were evaluated and compared with clinicopathological factors including overall survival. SOX9 expression was immunohistochemically evaluated in 382 GC tumors and the methylation status was examined in 121 GC tumors. SOX9 expression and its methylation status in six GC cell lines, their Epstein–Barr virus (EBV)-infected cell lines, and two EBV-associated GC cell lines was also examined. The SOX9 expression increased from non-neoplastic mucosa to early cancer. High expression of SOX9 was seen in 212 cases (56%). SOX9 expression was inversely related to advanced tumor stage, vessel infiltration, nodal metastasis, and EBV infection. Fifty-eight (48%) of 121 GC tumors had a methylated promoter in GC and the methylated status was related to low expression. The expression and methylation status were not related to prognosis. Three of six cell lines had increased methylation through EBV infection and decreased SOX9 expression. Upregulation of SOX9 is related to GC development. Downregulation by promoter methylation is related to GC progression and EBV infection. SOX9 is closely related to GC carcinogenesis and EBV-associated GC carcinogenesis.

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Title: SOX9 expression and its methylation status in gastric cancer
Authors: Minhua Sun
Hiroshi Uozaki
Rumi Hino
Akiko Kunita
Aya Shinozaki
Tetsuo Ushiku
Takashi Hibiya
Kimiko Takeshita
Maya Isogai
Kenzo Takada
Masashi Fukayama
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2012
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-012-1201-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

SOX9 expression and its methylation status in gastric cancer

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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