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01-09-2009 | Original Article

Immunohistochemical expression of ubiquitin and telomerase in cervical cancer

Authors: Toro de Méndez Morelva, Llombart Bosch Antonio

Published in: Virchows Archiv | Issue 3/2009

Abstract

Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue samples. Eighty-one cervical cancer cases and 22 normal exo–endocervical tissue were examined with polyclonal antibody for ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42.1%). Immunohistochemical findings were as follows: 98.7% of cervical cancers showed immunoexpression for ubiquitin and 52.6% for telomerase. Statistically significant differences were found in tumor immunoreactivity when compared with control tissue (p < 0.0007) for both biomarkers. There was no significant difference in biomarker expression at different histological types of tumors, although telomerase was less expressed in endocervical adenocarcinoma. Our findings confirm that abnormal immunoexpression pattern of ubiquitin and telomerase is common in HPV-positive cervical cancer, indicating the existence of an intense degradation of proteins, subsequent cellular immortalization and maintenance of the malignant phenotype.

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Title: Immunohistochemical expression of ubiquitin and telomerase in cervical cancer
Authors: Toro de Méndez Morelva
Llombart Bosch Antonio
Publication date: 01-09-2009
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2009
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-009-0818-7

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Immunohistochemical expression of ubiquitin and telomerase in cervical cancer

Abstract

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