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Virchows Archiv
Published in: Virchows Archiv 3/2009

Open Access 01-09-2009 | Original Article

High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancers

Authors: Tineke E. Buffart, Nicole C. T. van Grieken, Marianne Tijssen, Jordy Coffa, Bauke Ylstra, Heike I. Grabsch, Cornelis J. H. van de Velde, Beatriz Carvalho, Gerrit A. Meijer

Published in: Virchows Archiv | Issue 3/2009

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Abstract

DNA copy-number gains of chromosomes 8q, 13q, and 20q are frequently observed in gastric cancers. Moreover gain of chromosome 20q has been associated with lymph node metastasis. The aim of this study was to correlate DNA copy-number changes of individual genes on chromosomes 8q, 13q, and 20q in gastric adenocarcinomas to clinicopathological data. DNA isolated from 63 formalin-fixed and paraffin-embedded gastric adenocarcinoma tissue samples was analyzed by whole-genome microarray comparative genomic hybridization and by multiplex ligation-dependent probe amplification (MLPA), targeting 58 individual genes on chromosomes 8, 13, and 20. Using array comparative genomic hybridization, gains on 8q, 13q, and 20q were observed in 49 (77.8%), 25 (39.7%), and 49 (77.8%) gastric adenocarcinomas, respectively. Gain of chromosome 20q was significantly correlated with lymph node metastases (p = 0.05) and histological type (p = 0.02). MLPA revealed several genes to be frequently gained in DNA copy number. The oncogene c-myc on 8q was gained in 73% of the cancers, while FOXO1A and ATP7B on 13q were both gained in 28.6% of the cases. Multiple genes on chromosome 20q showed gains in more than 60% of the cancers. DNA copy-number gains of TNFRSF6B (20q13.3) and ZNF217 (20q13.2) were significantly associated with lymph node metastasis (p = 0.02) and histological type (p = 0.02), respectively. In summary, gains of chromosomes 8q, 13q, and 20q in gastric adenocarcinomas harbor DNA copy-number gains of known and putative oncogenes. ZNF217 and TNFRSF6B are associated with important clinicopathological variables, including lymph node status.
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Metadata
Title
High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancers
Authors
Tineke E. Buffart
Nicole C. T. van Grieken
Marianne Tijssen
Jordy Coffa
Bauke Ylstra
Heike I. Grabsch
Cornelis J. H. van de Velde
Beatriz Carvalho
Gerrit A. Meijer
Publication date
01-09-2009
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 3/2009
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-009-0814-y

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