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01-04-2008 | Original Article

Frequent epigenetic silencing of the FHIT gene in penile squamous cell carcinomas

Authors: Naoki Yanagawa, Mitsumasa Osakabe, Masahiro Hayashi, Gen Tamura, Teiichi Motoyama

Published in: Virchows Archiv | Issue 4/2008

Abstract

Methylation of normally unmethylated CpG-rich islands in or near the promoter region has been associated with transcriptional inactivation of tumor-suppressor and tumor-related genes in human cancers. However, so far, only a few studies have searched for DNA methylation in penile carcinoma (PC). On the other hand, human papillomavirus (HPV) has been reported to be associated with the pathogenesis of PC. To elucidate the methylation status of PC and HPV infection, the methylation status of eight genes (DAPK, FHIT, MGMT, p14, p16, RAR-β, RASSF1A, and RUNX3), the incidences of the HPV status, and the expression of Fhit protein were examined in 25 PCs. The frequencies of methylation were: 28% for DAPK, 92% for FHIT, 20% for MGMT, 4% for p14, 24% for p16, 24% for RAR-β, 12% for RASSF1A, and 44% for RUNX3. Negative expression of Fhit protein was observed in 22 of the 25 cancers (88%). Among those 22, 20 showed methylation of the FHIT gene. HPV-DNA was detected in three of the 25 cancers (12%). Methylation of FHIT gene was frequently found than HPV infection, therefore methylation of the FHIT gene is suggested to play an important role in the pathogenesis of penile squamous cell carcinoma.

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Title: Frequent epigenetic silencing of the FHIT gene in penile squamous cell carcinomas
Authors: Naoki Yanagawa
Mitsumasa Osakabe
Masahiro Hayashi
Gen Tamura
Teiichi Motoyama
Publication date: 01-04-2008
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 4/2008
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-008-0597-6

At a glance: The STEP trials

Springer Medicine

Frequent epigenetic silencing of the FHIT gene in penile squamous cell carcinomas

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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