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Virchows Archiv
Published in: Virchows Archiv 3/2006

01-09-2006 | Original Article

Oncofetal protein glypican-3 in testicular germ-cell tumor

Authors: Satoshi Ota, Michiyo Hishinuma, Naoko Yamauchi, Akiteru Goto, Teppei Morikawa, Tetsuya Fujimura, Tadaichi Kitamura, Tatsuhiko Kodama, Hiroyuki Aburatani, Masashi Fukayama

Published in: Virchows Archiv | Issue 3/2006

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Abstract

The expression of an oncofetal protein, the glypican-3 (GPC3), was immunohistochemically evaluated in a wide variety of primary testicular germ-cell tumors (GCTs) in comparison with other markers, alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG)-beta, and OCT3/4. Eighty-nine cases of GCT including 22 cases of mixed GCT were evaluated with reference to each tumor component. GPC3 expression was observed in neoplastic cells of yolk-sac tumor (YST) (25/25), teratoma (2/10), components of syncytiotrophoblastic giant cells (STGCs) (10/14), and choriocarcinoma (1/3), but none in intratubular germ-cell neoplasias, unclassified type (0/33), seminomas (0/61), or embryonal carcinoma (0/19). All cases of YST showed diffuse labeling of neoplastic cells in cytoplasmic and membranous patterns, and the positive area of GPC3 was much larger than that of AFP. Glandular structures in teratomas showed GPC3 immunostaining as well as AFP. Although the number of GPC3-positive cells was smaller in STGC components and choriocarcinoma, there was no diffusion artifact in GPC3 immunostaining, as was frequently encountered in hCG-beta staining. Thus, GPC3 is a unique oncofetal protein, which is useful as an immunohistochemical marker for GCT differentiated to extraembryonic tissue, especially YST.
Literature
1.
Cardoso de Almeida PC, Scully RE (1983) Diffuse embryoma of the testis. A distinctive form of mixed germ cell tumor. Am J Surg Pathol 7:633–642PubMedCrossRef
2.
De Cat B, Muyldermans SY, Coomans C, Degeest G, Vanderschueren B, Creemers J, Biemar F, Peers B, David G (2003) Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. J Cell Biol 163:625–635PubMedCrossRef
3.
de Peralta-Venturina MN, Ro JY, Ordonez NG, Ayala AG (1994) Diffuse embryoma of the testis. An immunohistochemical study of two cases. Am J Clin Pathol 102:402–405PubMed
4.
Duc-Goiran P, Mignot TM, Robert B, Machavoine F, Mondon F, Hagnere AM, Vacher-Lavenu MC, Danan JL, Vaiman D, Benassayag C, Ferre F (2005) Expression and Localization of Alpha-fetoprotein mRNA and Protein in Human Early Villous Trophoblasts. Placenta (in press)
5.
Eble JN, Sauter G, Epstein JI, Sesterhenn IA (eds) (2004) World Health Organization classification of tumors. Pathology and genetics tumours of the urinary system and male genital organs. IARC Press, pp 218–249
6.
7.
Grisaru S, Cano-Gauci D, Tee J, Filmus J, Rosenblum ND (2001) Glypican-3 modulates BMP- and FGF-mediated effects during renal branching morphogenesis. Dev Biol 231:31–46PubMedCrossRef
8.
Hechelhammer L, Storkel S, Odermatt B, Heitz PU, Jochum W (2003) Epidermal growth factor receptor is a marker for syncytiotrophoblastic cells in testicular germ cell tumors. Virchows Arch 443:28–31PubMedCrossRef
9.
Hippo Y, Watanabe K, Watanabe A, Midorikawa Y, Yamamoto S, Ihara S, Tokita S, Iwanari H, Ito Y, Nakano K, Nezu J, Tsunoda H, Yoshino T, Ohizumi I, Tsuchiya M, Ohnishi S, Makuuchi M, Hamakubo T, Kodama T, Aburatani H (2004) Identification of soluble NH2-terminal fragment of glypican-3 as a serological marker for early-stage hepatocellular carcinoma. Cancer Res 64:2418–2423PubMedCrossRef
10.
Hishinuma M, Ohashi K, Yamauchi N, Kashima K, Uozaki H, Ota S, Kodama T, Aburatani H, Fukayama M (2006) Heptocellular oncofetal protein, Glypican 3 is a sensitive marker for alpha-fetoprotein producing gastric carcinoma. Histopathology (in press)
11.
Hoei-Hansen CE, Nielsen JE, Almstrup K, Sonne SB, Graem N, Skakkebaek NE, Leffers H, Rajpert-De Meyts E (2004) Transcription factor AP-2gamma is a developmentally regulated marker of testicular carcinoma in situ and germ cell tumors. Clin Cancer Res 10:8521–8530PubMedCrossRef
12.
Honecker F, Stoop H, de Krijger RR, Chris Lau YF, Bokemeyer C, Looijenga LH (2004) Pathobiological implications of the expression of markers of testicular carcinoma in situ by fetal germ cells. J Pathol 203:849–857PubMedCrossRef
13.
Hori K, Uematsu K, Yasoshima H, Yamada A, Sakurai K, Ohya M (1997) Testicular seminoma with human chorionic gonadotropin production. Pathol Int 47:592–599PubMedCrossRef
14.
Jacobsen GK (1983) Alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG) in testicular germ cell tumours. A comparison of histologic and serologic occurrence of tumour markers. Acta Pathol Microbiol Immunol Scand [A] 91:183–190
15.
Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L (2004) OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma. Am J Surg Pathol 28:935–940PubMedCrossRef
16.
Kawakami T, Okamoto K, Sugihara H, Hattori T, Reeve AE, Ogawa O, Okada Y (2003) The roles of supernumerical X chromosomes and XIST expression in testicular germ cell tumors. J Urol 169:1546–1552PubMedCrossRef
17.
Lafuste P, Robert B, Mondon F, Danan JL, Rossi B, Duc-Goiran P, Mignot TM, Nunez EA, Benassayag C, Ferre F (2002) Alpha-fetoprotein gene expression in early and full-term human trophoblast. Placenta 23:600–612PubMedCrossRef
18.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW (2003) POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63:2244–2250PubMed
19.
Mostofi FK, Sesterhenn IA, Davis CJ Jr (1988) Developments in histopathology of testicular germ cell tumors. Semin Urol 6:171–188PubMed
20.
Sesterhenn IA, Weiss RB, Mostofi FK, Stablein DM, Rowland RG, Falkson G, Rivkind SE, Vogelzang NJ (1992) Prognosis and other clinical correlates of pathologic review in stage I and II testicular carcinoma a report from the Testicular Cancer Intergroup Study. J Clin Oncol 10:69–78PubMed
21.
Suzuki K, Tokue A, Kamiakito T, Kuriki K, Saito K, Tanaka A (2001) Predominant expression of fibroblast growth factor (FGF) 8, FGF4, and FGF receptor 1 in nonseminomatous and highly proliferative components of testicular germ cell tumors. Virchows Arch 439:616–621PubMed
22.
Talerman A, Haije WG, Baggerman L (1980) Serum alphafetoprotein (AFP) in patients with germ cell tumors of the gonads and extragonadal sites: correlation between endodermal sinus (yolk sac) tumor and raised serum AFP. Cancer 46:380–385PubMedCrossRef
23.
Ulbright TM (2005) Germ cell tumors of the gonads: a selective review emphasizing problems in differential diagnosis, newly appreciated, and controversial issues. Mod Pathol 18(Suppl 2):S61–S79PubMedCrossRef
24.
Yamauchi N, Watanabe A, Hishinuma M, Ohashi K, Midorikawa Y, Morishita Y, Niki T, Shibahara J, Mori M, Makuuchi M, Hippo Y, Kodama T, Iwanari H, Aburatani H, Fukayama M (2005) The glypican 3 oncofetal protein is a promising diagnostic marker for hepatocellular carcinoma. Mod Pathol 18:1591–1598PubMed
Metadata
Title
Oncofetal protein glypican-3 in testicular germ-cell tumor
Authors
Satoshi Ota
Michiyo Hishinuma
Naoko Yamauchi
Akiteru Goto
Teppei Morikawa
Tetsuya Fujimura
Tadaichi Kitamura
Tatsuhiko Kodama
Hiroyuki Aburatani
Masashi Fukayama
Publication date
01-09-2006
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 3/2006
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-006-0238-x

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