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01-12-2012 | Original Article

HTK-N, a modified HTK solution, decreases preservation injury in a model of microsteatotic rat liver transplantation

Authors: Qinlong Liu, Helge Bruns, Daniel Schultze, Yi Xue, Markus Zorn, Christa Flechtenmacher, Beate K. Straub, Ursula Rauen, Peter Schemmer

Published in: Langenbeck's Archives of Surgery | Issue 8/2012

Abstract

Background

Ischemia/reperfusion injury is an obstacle especially in steatotic livers, including those with steatosis induced by acute toxic stress. Recently, a modified histidine-tryptophan-ketoglutarate (HTK) solution, HTK-N, has been developed. This solution contains N-acetylhistidine, amino acids, and iron chelators. This study was designed to test the effects of HTK-N on preservation injury to rat livers after acute toxic injury.

Methods

Microvesicular steatosis was induced by a single dose of ethanol (8 g/kg BW). Livers were harvested and stored at 4 °C for 8 h with HTK or HTK-N before transplantation. Tissue and blood samples were taken at 1, 8, and 24 h after reperfusion to compare serum liver enzymes (aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase), standard histology, and immunohistochemistry for myeloperoxidase (MPO), caspase-3, and inducible nitric oxide synthase. Survival was compared after 1 week. For statistics, Analysis of Variance and t test were used.

Results

HTK-N improved survival from 12.5 % in HTK to 87.5 % (p < 0.05). Furthermore, liver enzymes were decreased to 2–75 % of HTK values (p < 0.05). Necrosis and leukocyte infiltration and MPO, caspase-3, and iNOS expression after transplantation were decreased (p < 0.05).

Conclusions

This study demonstrates that HTK-N protects liver grafts with microvesicular steatosis caused by acute toxic injury from cold ischemic injury better than standard HTK most likely via inhibition of hypoxic injury and oxidative stress and amelioration of the inflammatory reaction occurring upon reperfusion.

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Title: HTK-N, a modified HTK solution, decreases preservation injury in a model of microsteatotic rat liver transplantation
Authors: Qinlong Liu
Helge Bruns
Daniel Schultze
Yi Xue
Markus Zorn
Christa Flechtenmacher
Beate K. Straub
Ursula Rauen
Peter Schemmer
Publication date: 01-12-2012
Publisher: Springer-Verlag
Published in: Langenbeck's Archives of Surgery / Issue 8/2012
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-012-1022-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

HTK-N, a modified HTK solution, decreases preservation injury in a model of microsteatotic rat liver transplantation

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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