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Langenbeck's Archives of Surgery
Published in: Langenbeck's Archives of Surgery 3/2011

01-03-2011 | Original Article

Chronological evaluation of inflammatory mediators during peritoneal adhesion formation using a rat model

Authors: Marcel Binnebösel, Christian Daniel Klink, Julia Serno, Petra Lynen Jansen, Klaus Thilo von Trotha, Ulf Peter Neumann, Karsten Junge

Published in: Langenbeck's Archives of Surgery | Issue 3/2011

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Abstract

Purpose

The inflammatory response to peritoneal injury is considered to be of particular importance in adhesion formation. The aim of this study was to investigate the dynamics of inflammatory mediators in peritoneal adhesions.

Methods

In 60 male rats, a peritoneal defect was performed using a standardized cecal abrasion model. On days 3, 5, 14, 30, 60, and 90, ten animals were sacrificed. The expression of five integral mediators for the cellular immune response (macrophages, T lymphocytes), inflammation (COX-2), cell differentiation, and proliferation (ß-catenin, c-myc) in visceral and parietal adhesions were analyzed.

Results

A distinct infiltration of macrophages was observed in all animals up to the 90th postoperative day with a peak on day 3 for visceral adhesions (26.3 ± 5.6%) and on day 14 for parietal adhesions (5.1 ± 1.1%). Compared to parietal adhesions, macrophage levels were significantly higher on day 3 (p = 0.001) and 5 (p = 0.002) but significantly lower on days 30, 60, and 90 in visceral adhesions (p = 0.041; p = 0.001; p = 0.017). T lymphocytes were detected over time with the highest levels on day 3 (visceral 4.0 ± 0.7%; parietal 6.7 ± 2.9%). High levels of COX-2 expression could be detected for the whole observation period. Positive expression of both ß-catenin and c-myc was detected in persistent adhesions; however, no expression of c-myc was observed in parietal adhesions.

Conclusions

The inflammatory reaction in adhesions is not limited to the early postoperative phase. Macrophages may be fundamental in triggering adhesions, and the presence of T cells indicates an additional role of the adoptive immune system. Identification of chemokines and chemokine receptors that trigger the cellular immune response might be a potential option to minimize adhesion formation.
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Metadata
Title
Chronological evaluation of inflammatory mediators during peritoneal adhesion formation using a rat model
Authors
Marcel Binnebösel
Christian Daniel Klink
Julia Serno
Petra Lynen Jansen
Klaus Thilo von Trotha
Ulf Peter Neumann
Karsten Junge
Publication date
01-03-2011
Publisher
Springer-Verlag
Published in
Langenbeck's Archives of Surgery / Issue 3/2011
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI
https://doi.org/10.1007/s00423-011-0740-8

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