Top

Langenbeck's Archives of Surgery

Published in:

01-02-2010 | Original Article

Interactions of neutrophils with silver-coated vascular polyester grafts

Authors: Joerg Tautenhahn, Frank Meyer, Thomas Buerger, Uwe Schmidt, Hans Lippert, Wolfgang Koenig, Brigitte Koenig

Published in: Langenbeck's Archives of Surgery | Issue 2/2010

Abstract

Background

In reconstructive vascular surgery, infection is one of the most feared complications because of the high mortality. While the antimicrobial effect of a silver-coated endoprosthesis has been proven in experimental trials, there are no reports on its interactions with granulocytes, the first effector cells in general inflammation and in infection.

Materials and methods

Therefore, we investigated whether silver coating of vascular polyester grafts affects receptor expression, mediator release, and functions of human neutrophils relevant for microbicidal activity and the wound-healing process. Naïve neutrophils were analyzed for their cellular receptors such as cluster of differentiation (CD)62L, CD11b, CXCR2, and fMLP-R, the mediators interleukin 8, granulocyte elastase (human neutrophil elastase), and leukotriene B4 (LTB4) as well as for microbicidal capacity (oxidative burst) in vitro. In addition, the role of plasma coating for receptor expression was addressed.

Results

There was both a decrease of CD62L and CXCR2 expression and an increase of CD11b, fMLP-R expression, elastase release, and LTB4 generation, which were statistically significant (p = 0.04; p = 0.01; p = 0.0; p = 0.0; p = 0.01; p = 0.02, respectively) in the presence of the silver-coated graft compared with non-silver-coated vascular grafts. In addition, microbicidal activity was significantly (p = 0.0) impaired by the silver-coated graft. Coating of the vascular grafts with plasma did not alter the former observations significantly.

Conclusion

The results may indicate that silver-coated vascular polyester grafts activate neutrophils chronically which may favor tissue destruction and impaired antimicrobial effects.

Goëau-Brissonniére OA, Coggia M (2000) Arterial prosthetic infections. In: Waldvogel FA, Bisno AL (eds) Infections associated with indwelling medical devices. ASM, Washington, pp 127–143

Benvenisty AI, Tannenbaum G, Ahlborn TN et al (1999) Control of prosthetic bacterial infection: evaluation of an easily incorporated, tightly bound, silver antibiotic PTFE graft. J Surg Res 44:1–7CrossRef

Hollinger MA (1996) Toxicological aspects of topical silver pharmaceuticals. Crit Rev Toxicol 26:255–260CrossRefPubMed

Burg ND, Pillinger MH (2001) The neutrophil: function and regulation in innate and humoral immunity. Clin Immunol 99:7–17CrossRefPubMed

Adams JM, Hauser CJ, Livingston DH et al (2001) Early trauma polymorphonuclear neutrophil responses to chemokines are associated with development of sepsis, pneumonia, and organ failure. J Trauma 51:452–456CrossRefPubMed

de la Riviere AB, Dossche KME, Birnbaum DE et al (2000) First clinical experience with a mechanical valve with silver coating. J Heart Valve Dis 9:123–130

Hughes SF, Cotter MJ, Evans SA et al (2006) Role of leucocytes in damage to vascular endothelium during ischaemia–reperfusion injury. Br J Biomed Sci 63:166–170PubMed

Lappegard KT, Bergseth G, Riesenfeld J et al (2005) Role of granulocytes and monocytes in the polyvinyl chloride-induced synthesis of interleukin 8, monocyte chemoattractant protein 1, and leukotriene B4. J Biomed Mat Res A 74:230–236CrossRef

Toft P, Krog J, Brix-Christensen V et al (2000) The effect of CVVHD and endotoxin on the oxidative burst, adhesion molecules and distribution in tissue of granulocytes. Intensive Care Med 26:770–775CrossRefPubMed

10.

Jakubiec B, Roy R, Isles MB et al (1994) Measurement of CD11/CD18 integrin expression on the polymorphonuclear cell surface after incubation with synthetic vascular prostheses. ASIO J 40:M616–M618CrossRef

11.

Swartbol P, Truedsson L, Parsson H et al (1996) Surface adhesion molecule expression on human blood cells induced by vascular graft materials in vitro. J Biomed Mater Res 32:669–676CrossRefPubMed

12.

Gorbet MB, Yeo EL, Sefton MV (1999) Flow cytometric study of in vitro activation by biomaterials. Biomed Mater Res 44:289–297CrossRef

13.

Chang CC, Lieberman SM, Moghe PV (1999) Leukocyte spreading behavior on vascular biomaterial surfaces: consequences of chemoattractant stimulation. Biomaterials 20:273–281CrossRefPubMed

14.

Ley K (2002) Integration of inflammatory signals by rolling neutrophils. Immunol Rev 186:8–18CrossRefPubMed

15.

Graves V, Gabig T, McCarthy L et al (1993) Simultaneous mobilization of Mac-1 (CD11b/CD18) and formyl peptide chemoattractant receptors in human neutrophils. Blood 81:1668

16.

Chuntharapai A, Lee J, Hébert CA et al (1994) Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J Immunol 153:5682–5688PubMed

17.

Pärsson H, Nassberer L, Thorne J et al (1995) Metabolic response of granulocytes and platelets to synthetic vascular grafts: preliminary results with an in vitro technique. J Biomed Mater Res 29:519–525CrossRefPubMed

18.

Sabroe I, Williams TJ, Hébert CA et al (1997) Chemoattractant cross-desensitization of the human neutrophil IL-8 receptor involves receptor internalization and differential receptor subtype regulation. J Immunol 158:1361–1369PubMed

19.

Bullard DC (2002) Adhesion molecules in inflammatory diseases: insights from knockout mice. Immunol Res 26:27–33CrossRefPubMed

20.

Falkenback D, Lundberg F, Ribbe E et al (2000) Exposure of plasma proteins on Dacron and ePTFE vascular graft material in a perfusion model. Eur J Vasc Endovasc Surg 19:468–475CrossRefPubMed

21.

Kansas GS (1996) Selectins and their ligands: current concepts and controversies. Blood 88:3259–3287PubMed

22.

Kasama T, Miwa Y, Isozaki T et al (2005) Neutrophil-derived cytokines: potential therapeutic targets in inflammation. Curr Drug Targets Inflamm Allergy 4:273–279CrossRefPubMed

23.

Chishti AD, Dark JH, Kesteven P et al (2001) Expression of chemokine receptors CXCR1 and CXCR2 during cardiopulmonary bypass. J Thorac Cardiovasc Surg 122:1162–1167CrossRefPubMed

24.

Cummings CJ, Martin TR, Frevert CW et al (1999) Expression and function of the chemokine receptors CXCR2 and CXCR2 in sepsis. J Immunol 162:2341–2346PubMed

25.

Freytag CC, Tautenhahn J, Koenig W, Lippert H, Bürger T (2003) Ultrastructural analysis of an infected collagen-coated vascular graft. Vasa 32:31–35CrossRefPubMed

26.

Vaudaux P, Francois P, Lew DP et al (2000) Host factors predisposing to influencing therapy of foreign body infections. In: Waldvogel FA, Bisno AL (eds) Infections associated with indwelling medical devices. ASM, Washington, pp 1–26

27.

De La Cruz C, Haimovich B, Greco RS (1998) Immobilized IgG and fibrinogen differentially affect the cytoskeletal organization and bacterial function of adherent neutrophils. J Surg Res 80:28–34CrossRefPubMed

28.

Munch K, Wolf MF, Gruffaz P (2000) Use of simple and complex in vitro models for multiparameter characterization of human blood-material/device interactions. J Biomater Sci Polym Ed 11:1147–1163CrossRefPubMed

29.

Poon V, Burd A (2004) in vitro cytotoxicity of silver: implication for clinical wound care. Burns 30:140–147CrossRefPubMed

30.

Rahimi K, Maerz HK, Zotz RJ et al (2003) Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease. Cytomety B Clin Cytom 53:63–69CrossRef

31.

Roon AJ, Malone MJ, Moore WS et al (1977) Bacteremic infectability: a function of vascular graft material and design. J Surg Res 22:489–498CrossRefPubMed

32.

Trautinger F, Hammerle AF, Pöschl G et al (1991) Respiratory burst capability of polymorphonuclear neutrophils and TNF-α serum levels in relationship to the development of septic syndrome in critically ill patients. J Leukoc Biol 49:449–454PubMed

33.

Hernandez-Richter T, Wittmann B, Wittmann F, Loehe F, Rentsch M, Mayr S, Jauch KW, Angele MK (2007) Titanium-coated silicone is not effective for preventing graft infection. Zentralbl Chir 132:32–37CrossRefPubMed

Title: Interactions of neutrophils with silver-coated vascular polyester grafts
Authors: Joerg Tautenhahn
Frank Meyer
Thomas Buerger
Uwe Schmidt
Hans Lippert
Wolfgang Koenig
Brigitte Koenig
Publication date: 01-02-2010
Publisher: Springer-Verlag
Published in: Langenbeck's Archives of Surgery / Issue 2/2010
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-008-0439-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Interactions of neutrophils with silver-coated vascular polyester grafts

Abstract

Background

Materials and methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 2/2010

Reoperation for primary hyperparathyroidism: tips and tricks

Energy-based hemostatic devices in laparoscopic adrenalectomy

Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis

Long-term effects of surgical correction of adrenal hyperplasia and adenoma causing primary aldosteronism

Bipolar thermofusion vessel sealing system (TVS) versus conventional vessel ligation (CVL) in thyroid surgery—results of a prospective study

Electromyographic response persists after peripheral transection: endorsement of current concepts in recurrent laryngeal nerve monitoring in a porcine model