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Langenbeck's Archives of Surgery
Published in: Langenbeck's Archives of Surgery 3/2007

01-05-2007 | Original Article

Alterations of neuropeptides in the human gut during peritonitis

Authors: P. Jacob, M. H. Mueller, J. Hahn, I. Wolk, P. Mayer, U. Nagele, J. Hennenlotter, A. Stenzl, A. Konigsrainer, J. Glatzle

Published in: Langenbeck's Archives of Surgery | Issue 3/2007

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Abstract

Background and aims

Gastrointestinal motility is reduced during sepsis but the pathomechanism involved is poorly understood. We investigated the expression of substance P (SP) and vasoactive intestinal peptide (VIP) in the myenteric plexus during peritonitis in human small bowel.

Materials and methods

Tissue samples of the small bowel were gathered from healthy patients and from patients with peritonitis. Immunohistochemistry for myeloperoxidase (MPO), SP, and VIP was performed in whole mount sections. To determine the level of inflammation, MPO-positive cells were counted in the circular muscle layer. SP and VIP immunoreactivity was analyzed in myenteric plexus neurons. The area of positive immunoreactivity for either neuropeptide within the plexus was analyzed and set in relation to the total area of the plexus and consecutively expressed as percentage.

Results

During peritonitis, MPO-positive cells significantly increased by approximately fourfold as compared to healthy tissue. The immunoreactivity for SP was significantly reduced by approximately 80% in myenteric plexus neurons during peritonitis. In contrast, the immunoreactivity for VIP significantly increased by nearly twofold during peritonitis.

Conclusions

During peritonitis, the inflammatory reaction within the gut is increased. The neuropeptide expression in myenteric plexus neurons was observed as shifting towards increased expression of VIP, known to inhibit intestinal motility, and towards decreased expression of the prokinetic neuropeptide SP.
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Metadata
Title
Alterations of neuropeptides in the human gut during peritonitis
Authors
P. Jacob
M. H. Mueller
J. Hahn
I. Wolk
P. Mayer
U. Nagele
J. Hennenlotter
A. Stenzl
A. Konigsrainer
J. Glatzle
Publication date
01-05-2007
Publisher
Springer-Verlag
Published in
Langenbeck's Archives of Surgery / Issue 3/2007
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI
https://doi.org/10.1007/s00423-007-0168-3

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