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27-03-2024 | Original Article

Circulating catecholamines, endothelin-1, and nitric oxide releases do not explain the preserved FMD following acute resistance exercise in strength-trained men

Authors: Takuma Morishima, Nobukazu Kasai

Published in: European Journal of Applied Physiology

Abstract

Purpose

Acute resistance exercise decreases endothelial function in sedentary individuals but not in strength-trained (ST) individuals. However, the underlying mechanism(s) of vascular protection in ST individuals remains unclear. Herein, we compared catecholamines, endothelin-1 (ET-1), and nitric oxide (NOx) releases after acute resistance exercise between sedentary and ST individuals.

Methods

The untrained (UT) group comprised 12 male individuals with no regular training, while the ST group comprised 12 male individuals. Participants performed a session of resistance exercise, which consisted of 3 sets of 10 repetitions at 75% of one repetition maximum. Heart rate (HR) and blood pressure were measured during resistance exercise. Brachial artery flow-mediated dilation (FMD), blood pressure, HR, and blood collection were undertaken before and 10, 30, and 60 min after the resistance exercise.

Results

No significant difference was found in baseline brachial artery FMD between the groups (P > 0.05). Brachial artery FMD was significantly reduced in the UT group (P < 0.05) but it was prevented in the ST group after the resistance exercise. Significant differences were found at 10, 30, and 60 min after the resistance exercise in brachial artery ΔFMD from baseline between groups (P < 0.05). Blood pressure, HR, plasma epinephrine, norepinephrine, dopamine, serum endothelin-1, and plasma NOx responses did not differ between groups throughout the experimental period.

Conclusion

In conclusion, preserved endothelial function in response to acute resistance exercise in ST male individuals is independent of catecholamines, ET-1, and NOx responses.

Atkinson G, Batterham AM, Thijssen DH, Green DJ (2013) A new approach to improve the specificity of flow-mediated dilation for indicating endothelial function in cardiovascular research. J Hypertens 31(2):287–291. https://doi.org/10.1097/HJH.0b013e32835b8164CrossRefPubMed

Atkinson CL, Lewis NC, Carter HH, Thijssen DH, Ainslie PN, Green DJ (2015) Impact of sympathetic nervous system activity on post-exercise flow-mediated dilatation in humans. J Physiol 593(23):5145–5156. https://doi.org/10.1113/JP270946CrossRefPubMedPubMedCentral

Bartak V, Vybiral S, Papezova H, Dostalova I, Pacak K, Nedvidkova J (2004) Basal and exercise-induced sympathetic nervous activity and lipolysis in adipose tissue of patients with anorexia nervosa. Eur J Clin Invest 34(5):371–377. https://doi.org/10.1111/j.1365-2362.2004.01344.xCrossRefPubMed

Boeno FP, Farinha JB, Ramis TR, Macedo RCO, Rodrigues-Krause J, do Nascimento QJ, Lopez P, Pinto RS, Reischak-Oliveira A (2019) Effects of a single session of high- and moderate-intensity resistance exercise on endothelial function of middle-aged sedentary men. Front Physiol 10:777. https://doi.org/10.3389/fphys.2019.00777CrossRefPubMedPubMedCentral

Boyle LJ, Credeur DP, Jenkins NT, Padilla J, Leidy HJ, Thyfault JP, Fadel PJ (2013) Impact of reduced daily physical activity on conduit artery flow-mediated dilation and circulating endothelial microparticles. J Appl Physiol 115(10):1519–1525. https://doi.org/10.1152/japplphysiol.00837.2013CrossRefPubMedPubMedCentral

Buchanan CE, Kadlec AO, Hoch AZ, Gutterman DD, Durand MJ (2017) Hypertension during weight lifting reduces flow-mediated dilation in nonathletes. Med Sci Sports Exerc 49(4):669–675. https://doi.org/10.1249/MSS.0000000000001150CrossRefPubMedPubMedCentral

Ceriello A, Quagliaro L, Piconi L, Assaloni R, Da Ros R, Maier A, Esposito K, Giugliano D (2004) Effect of postprandial hypertriglyceridemia and hyperglycemia on circulating adhesion molecules and oxidative stress generation and the possible role of simvastatin treatment. Diabetes 53(3):701–710. https://doi.org/10.2337/diabetes.53.3.701CrossRefPubMed

Dawson EA, Cable NT, Green DJ, Thijssen DHJ (2018) Do acute effects of exercise on vascular function predict adaptation to training? Eur J Appl Physiol 118(3):523–530. https://doi.org/10.1007/s00421-017-3724-8CrossRefPubMed

Dick GM, Katz PS, Farias M 3rd, Morris M, James J, Knudson JD, Tune JD (2006) Resistin impairs endothelium-dependent dilation to bradykinin, but not acetylcholine, in the coronary circulation. Am J Physiol Heart Circ Physiol 291(6):H2997-3002. https://doi.org/10.1152/ajpheart.01035.2005CrossRefPubMed

Dimsdale JE, Moss J (1980) Plasma catecholamines in stress and exercise. JAMA 243(4):340–342CrossRefPubMed

Durand MJ, Dharmashankar K, Bian JT, Das E, Vidovich M, Gutterman DD, Phillips SA (2015) Acute exertion elicits a H2O2-dependent vasodilator mechanism in the microvasculature of exercise-trained but not sedentary adults. Hypertension 65(1):140–145. https://doi.org/10.1161/HYPERTENSIONAHA.114.04540CrossRefPubMed

Fairfax ST, Padilla J, Vianna LC, Holwerda SW, Davis MJ, Fadel PJ (2015) Myogenic responses occur on a beat-to-beat basis in the resting human limb. Am J Physiol Heart Circ Physiol 308(5):H554-555. https://doi.org/10.1152/ajpheart.00012.2015CrossRefPubMed

Finaud J, Lac G, Filaire E (2006) Oxidative stress : relationship with exercise and training. Sports Med 36(4):327–358. https://doi.org/10.2165/00007256-200636040-00004CrossRefPubMed

Gentile MT, Vecchione C, Marino G, Aretini A, Di Pardo A, Antenucci G, Maffei A, Cifelli G, Iorio L, Landolfi A, Frati G, Lembo G (2008) Resistin impairs insulin-evoked vasodilation. Diabetes 57(3):577–583. https://doi.org/10.2337/db07-0557CrossRefPubMed

Green DJ, Maiorana A, O’Driscoll G, Taylor R (2004) Effect of exercise training on endothelium-derived nitric oxide function in humans. J Physiol 561(Pt 1):1–25. https://doi.org/10.1113/jphysiol.2004.068197CrossRefPubMedPubMedCentral

Green DJ, Jones H, Thijssen D, Cable NT, Atkinson G (2011) Flow-mediated dilation and cardiovascular event prediction: does nitric oxide matter? Hypertension 57(3):363–369. https://doi.org/10.1161/HYPERTENSIONAHA.110.167015CrossRefPubMed

Han SH, Quon MJ, Kim JA, Koh KK (2007) Adiponectin and cardiovascular disease: response to therapeutic interventions. J Am Coll Cardiol 49(5):531–538. https://doi.org/10.1016/j.jacc.2006.08.061CrossRefPubMed

Hasegawa N, Fujie S, Horii N, Miyamoto-Mikami E, Tsuji K, Uchida M, Hamaoka T, Tabata I, Iemitsu M (2018) Effects of different exercise modes on arterial stiffness and nitric oxide synthesis. Med Sci Sports Exerc 50(6):1177–1185. https://doi.org/10.1249/MSS.0000000000001567CrossRefPubMed

Ignarro LJ (2002) Nitric oxide as a unique signaling molecule in the vascular system: a historical overview. J Physiol Pharmacol 53(4 Pt 1):503–514PubMed

Ikeda N, Yasu T, Tsuboi K, Sugawara Y, Kubo N, Umemoto T, Arao K, Kawakami M, Momomura S (2010) Effects of submaximal exercise on blood rheology and sympathetic nerve activity. Circ J 74(4):730–734. https://doi.org/10.1253/circj.cj-09-0758CrossRefPubMed

Joyner MJ, Green DJ (2009) Exercise protects the cardiovascular system: effects beyond traditional risk factors. J Physiol 587(Pt 23):5551–5558. https://doi.org/10.1113/jphysiol.2009.179432CrossRefPubMedPubMedCentral

Jurva JW, Phillips SA, Syed AQ, Syed AY, Pitt S, Weaver A, Gutterman DD (2006) The effect of exertional hypertension evoked by weight lifting on vascular endothelial function. J Am Coll Cardiol 48(3):588–589. https://doi.org/10.1016/j.jacc.2006.05.004CrossRefPubMed

Kawano H, Motoyama T, Hirashima O, Hirai N, Miyao Y, Sakamoto T, Kugiyama K, Ogawa H, Yasue H (1999) Hyperglycemia rapidly suppresses flow-mediated endothelium-dependent vasodilation of brachial artery. J Am Coll Cardiol 34(1):146–154. https://doi.org/10.1016/s0735-1097(99)00168-0CrossRefPubMed

Kobayashi H, Ouchi N, Kihara S, Walsh K, Kumada M, Abe Y, Funahashi T, Matsuzawa Y (2004) Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin. Circ Res 94(4):e27-31. https://doi.org/10.1161/01.RES.0000119921.86460.37CrossRefPubMedPubMedCentral

Lamping KG, Dole WP (1987) Acute hypertension selectively potentiates constrictor responses of large coronary arteries to serotonin by altering endothelial function in vivo. Circ Res 61(6):904–913. https://doi.org/10.1161/01.res.61.6.904CrossRefPubMed

Maeda S, Miyauchi T, Kakiyama T, Sugawara J, Iemitsu M, Irukayama-Tomobe Y, Murakami H, Kumagai Y, Kuno S, Matsuda M (2001) Effects of exercise training of 8 weeks and detraining on plasma levels of endothelium-derived factors, endothelin-1 and nitric oxide, in healthy young humans. Life Sci 69(9):1005–1016. https://doi.org/10.1016/s0024-3205(01)01192-4CrossRefPubMed

Mansouri M, Keshtkar A, Hasani-Ranjbar S, Soleymani Far E, Tabatabaei-Malazy O, Omidfar K, Larijani B (2011) The impact of one session resistance exercise on plasma adiponectin and RBP4 concentration in trained and untrained healthy young men. Endocr J 58(10):861–868. https://doi.org/10.1507/endocrj.ej11-0046CrossRefPubMed

Morishima T, Padilla J, Tsuchiya Y, Ochi E (2020) Maintenance of endothelial function following acute resistance exercise in females is associated with a tempered blood pressure response. J Appl Physiol 129(4):792–799. https://doi.org/10.1152/japplphysiol.00378.2020CrossRefPubMed

Morishima T, Tsuchiya Y, Iemitsu M, Ochi E (2018) High-intensity resistance exercise with low repetitions maintains endothelial function. Am J Physiol Heart Circ Physiol 315(3):H681–H686. https://doi.org/10.1152/ajpheart.00281.2018CrossRefPubMed

Morishima T, Iemitsu M, Ochi E (2019a) Short-term cycling restores endothelial dysfunction after resistance exercise. Scand J Med Sci Sports 29(8):1115–1120. https://doi.org/10.1111/sms.13434CrossRefPubMed

Morishima T, Toyoda M, Ochi E (2019b) Prior cycling exercise does not prevent endothelial dysfunction after resistance exercise. Eur J Appl Physiol 119(7):1663–1669. https://doi.org/10.1007/s00421-019-04154-1CrossRefPubMed

Nishiyama SK, Zhao J, Wray DW, Richardson RS (2017) Vascular function and endothelin-1: tipping the balance between vasodilation and vasoconstriction. J Appl Physiol 122(2):354–360. https://doi.org/10.1152/japplphysiol.00772.2016CrossRefPubMed

Okamoto T, Masuhara M, Ikuta K (2008) Relationship between plasma endothelin-1 concentration and cardiovascular responses during high-intensity eccentric and concentric exercise. Clin Physiol Funct Imaging 28(1):43–48. https://doi.org/10.1111/j.1475-097X.2007.00775.xCrossRefPubMed

Phillips SA, Das E, Wang J, Pritchard K, Gutterman DD (2011) Resistance and aerobic exercise protects against acute endothelial impairment induced by a single exposure to hypertension during exertion. J Appl Physiol 110(4):1013–1020. https://doi.org/10.1152/japplphysiol.00438.2010CrossRefPubMedPubMedCentral

Raitakari OT, Celermajer DS (2000) Flow-mediated dilatation. Br J Clin Pharmacol 50(5):397–404. https://doi.org/10.1046/j.1365-2125.2000.00277.xCrossRefPubMedPubMedCentral

Restaino RM, Walsh LK, Morishima T, Vranish JR, Martinez-Lemus LA, Fadel PJ, Padilla J (2016) Endothelial dysfunction following prolonged sitting is mediated by a reduction in shear stress. Am J Physiol Heart Circ Physiol 310(5):H648-653. https://doi.org/10.1152/ajpheart.00943.2015CrossRefPubMedPubMedCentral

Shechter M, Issachar A, Marai I, Koren-Morag N, Freinark D, Shahar Y, Shechter A, Feinberg MS (2009) Long-term association of brachial artery flow-mediated vasodilation and cardiovascular events in middle-aged subjects with no apparent heart disease. Int J Cardiol 134(1):52–58. https://doi.org/10.1016/j.ijcard.2008.01.021CrossRefPubMed

Tanasescu M, Leitzmann MF, Rimm EB, Willett WC, Stampfer MJ, Hu FB (2002) Exercise type and intensity in relation to coronary heart disease in men. JAMA 288(16):1994–2000. https://doi.org/10.1001/jama.288.16.1994CrossRefPubMed

Thijssen DH, Dawson EA, Tinken TM, Cable NT, Green DJ (2009) Retrograde flow and shear rate acutely impair endothelial function in humans. Hypertension 53(6):986–992. https://doi.org/10.1161/HYPERTENSIONAHA.109.131508CrossRefPubMed

Thijssen DH, Black MA, Pyke KE, Padilla J, Atkinson G, Harris RA, Parker B, Widlansky ME, Tschakovsky ME, Green DJ (2011) Assessment of flow-mediated dilation in humans: a methodological and physiological guideline. Am J Physiol Heart Circ Physiol 300(1):H2-12. https://doi.org/10.1152/ajpheart.00471.2010CrossRefPubMed

Thijssen DHJ, Bruno RM, van Mil A, Holder SM, Faita F, Greyling A, Zock PL, Taddei S, Deanfield JE, Luscher T, Green DJ, Ghiadoni L (2019) Expert consensus and evidence-based recommendations for the assessment of flow-mediated dilation in humans. Eur Heart J 40(30):2534–2547. https://doi.org/10.1093/eurheartj/ehz350CrossRefPubMed

Title LM, Cummings PM, Giddens K, Nassar BA (2000) Oral glucose loading acutely attenuates endothelium-dependent vasodilation in healthy adults without diabetes: an effect prevented by vitamins C and E. J Am Coll Cardiol 36(7):2185–2191. https://doi.org/10.1016/s0735-1097(00)00980-3CrossRefPubMed

Varady KA, Bhutani S, Church EC, Phillips SA (2010) Adipokine responses to acute resistance exercise in trained and untrained men. Med Sci Sports Exerc 42(3):456–462. https://doi.org/10.1249/MSS.0b013e3181ba6dd3CrossRefPubMed

Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332(6163):411–415. https://doi.org/10.1038/332411a0CrossRefPubMed

Title: Circulating catecholamines, endothelin-1, and nitric oxide releases do not explain the preserved FMD following acute resistance exercise in strength-trained men
Authors: Takuma Morishima
Nobukazu Kasai
Publication date: 27-03-2024
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-024-05468-5

At a glance: The STEP trials

Springer Medicine

Circulating catecholamines, endothelin-1, and nitric oxide releases do not explain the preserved FMD following acute resistance exercise in strength-trained men

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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