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European Journal of Applied Physiology
Published in: European Journal of Applied Physiology 1/2018

01-01-2018 | Original Article

Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis

Authors: Roksana B. Zak, B. M. Hassenstab, L. K. Zuehlke, M. W. S. Heesch, R. J. Shute, T. L. Laursen, D. T. LaSalle, D. R. Slivka

Published in: European Journal of Applied Physiology | Issue 1/2018

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Abstract

Purpose

To determine the impact of local muscle heating and cooling on myogenic and proteolytic gene responses following resistance exercise.

Methods

Recreationally trained males (n = 12), age 25.3 ± 1.5, % body fat 13.6 ± 1.92, completed four sets of 8–12 repetitions of unilateral leg press and leg extension while heating one leg, and cooling the other. Muscle biopsies were taken from the vastus lateralis of each leg pre and 4 h post exercise.

Results

MyoD, FOXO1, and MuRF1 mRNA increased with exercise regardless of temperature (p < 0.05). Myostatin, MYF5, and atrogin-1 mRNA decreased with exercise regardless of temperature (p < 0.05). Myogenin, MRF4, and CASP3 mRNA were higher in the hot condition, compared to the cold (p < 0.05). PAX7 mRNA was lower in the hot compared to cold condition (p = 0.041). FOXO3 mRNA was higher in the cold compared to hot condition (p = 0.037). AKT1 and AKT2 were unaffected by either exercise or temperature. Femoral artery blood flow volume was higher in the hot (375.2 ± 41.2 ml min− 1), compared to the cold condition (263.5 ± 23.9 ml min− 1), p = 0.01. Tissue oxygen saturation was higher in the hot (71.7 ± 4.8%) than cold condition (55.3 ± 5.0%).

Conclusion

These results suggest an impaired muscle growth response with local cold application compared to local heat application.

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Metadata
Title
Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis
Authors
Roksana B. Zak
B. M. Hassenstab
L. K. Zuehlke
M. W. S. Heesch
R. J. Shute
T. L. Laursen
D. T. LaSalle
D. R. Slivka
Publication date
01-01-2018
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Applied Physiology / Issue 1/2018
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI
https://doi.org/10.1007/s00421-017-3749-z

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