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01-05-2012 | Original Article

Skeletal muscle signaling response to sprint exercise in men and women

Authors: Teresa Fuentes, Borja Guerra, Jesús G. Ponce-González, David Morales-Alamo, Amelia Guadalupe-Grau, Hugo Olmedillas, Lorena Rodríguez-García, David Feijoo, Pedro De Pablos-Velasco, Leandro Fernández-Pérez, Alfredo Santana, Jose A. L. Calbet

Published in: European Journal of Applied Physiology | Issue 5/2012

Abstract

To determine if there is a sex dimorphism in the skeletal muscle signaling response to sprint exercise, 17 men and ten women performed a 30-s Wingate test. Muscle biopsies were taken before, immediately after the exercise and at 30 and 120 min during the recovery period. Thr¹⁷²-AMPKα, Ser²²¹-ACCβ, Thy⁷⁰⁵-STAT3, Thr²⁰²/Thy²⁰⁴-ERK1/2 and Thr¹⁸⁰/Thy¹⁸²-p38MAPK phosphorylation responses to sprint exercise were not statistically different between men and women. AMPKα phosphorylation was enhanced fourfold 30 min after the sprint exercise in males and females (P < 0.01). ACCβ phosphorylation was enhanced by about threefold just after the sprint test exercise and 30 min into the recovery period in males and females (P < 0.01). STAT3 phosphorylation was increased 2 h after the Wingate test compared to the value observed right after the end of the exercise (P < 0.05), and 30 min after the Wingate test there was a 2.5-fold increase in ERK1/2 phosphorylation, compared to both the pre-exercise and to the value observed right after the Wingate test (both, P < 0.05). In conclusion, the skeletal muscle signaling response to a single bout of sprint exercise mediated by AMPK, ACC, STAT3, ERK and p38MAPK is not statistically different between men and women. Marked increases in AMPKα, ACCβ, STAT3 and ERK phosphorylation were observed after a single 30-s all-out sprint (Wingate test) in the vastus lateralis.

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Title: Skeletal muscle signaling response to sprint exercise in men and women
Authors: Teresa Fuentes
Borja Guerra
Jesús G. Ponce-González
David Morales-Alamo
Amelia Guadalupe-Grau
Hugo Olmedillas
Lorena Rodríguez-García
David Feijoo
Pedro De Pablos-Velasco
Leandro Fernández-Pérez
Alfredo Santana
Jose A. L. Calbet
Publication date: 01-05-2012
Publisher: Springer-Verlag
Published in: European Journal of Applied Physiology / Issue 5/2012
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-011-2164-0

At a glance: The STEP trials

Springer Medicine

Skeletal muscle signaling response to sprint exercise in men and women

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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