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01-01-2011 | Original Article

Responses of muscle mass, strength and gene transcripts to long-term heat stress in healthy human subjects

Authors: Katsumasa Goto, Hideshi Oda, Hidehiko Kondo, Michihito Igaki, Atsushi Suzuki, Shuichi Tsuchiya, Takatoshi Murase, Tadashi Hase, Hiroto Fujiya, Ichiro Matsumoto, Hisashi Naito, Takao Sugiura, Yoshinobu Ohira, Toshitada Yoshioka

Published in: European Journal of Applied Physiology | Issue 1/2011

Abstract

The present study was performed to investigate the effects of long-term heat stress on mass, strength and gene expression profile of human skeletal muscles without exercise training. Eight healthy men were subjected to 10-week application of heat stress, which was performed for the quadriceps muscles for 8 h/day and 4 days/week by using a heat- and steam-generating sheet. Maximum isometric force during knee extension of the heated leg significantly increased after heat stress (~5.8%, P < 0.05). Mean cross-sectional areas (CSAs) of vastus lateralis (VL, ~2.7%) and rectus femoris (~6.1%) muscles, as well as fiber CSA (8.3%) in VL, in the heated leg were also significantly increased (P < 0.05). Statistical analysis of microarrays (SAM) revealed that 10 weeks of heat stress increased the transcript level of 925 genes and decreased that of 1,300 genes, and gene function clustering analysis (Database for Annotation, Visualization and Integrated Discovery: DAVID) showed that these regulated transcripts stemmed from diverse functional categories. Transcript level of ubiquinol-cytochrome c reductase binding protein (UQCRB) was significantly increased by 10 weeks of heat stress (~3.0 folds). UQCRB is classified as one of the oxidative phosphorylation-associated genes, suggesting that heat stress can stimulate ATP synthesis. These results suggested that long-term application of heat stress could be effective in increasing the muscle strength associated with hypertrophy without exercise training.

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Title: Responses of muscle mass, strength and gene transcripts to long-term heat stress in healthy human subjects
Authors: Katsumasa Goto
Hideshi Oda
Hidehiko Kondo
Michihito Igaki
Atsushi Suzuki
Shuichi Tsuchiya
Takatoshi Murase
Tadashi Hase
Hiroto Fujiya
Ichiro Matsumoto
Hisashi Naito
Takao Sugiura
Yoshinobu Ohira
Toshitada Yoshioka
Publication date: 01-01-2011
Publisher: Springer-Verlag
Published in: European Journal of Applied Physiology / Issue 1/2011
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-010-1617-1

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Responses of muscle mass, strength and gene transcripts to long-term heat stress in healthy human subjects

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